Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Removal of Aoueous Pentachlorophenol by Horseradish Peroxidase in the Presence of Surfactants

  • Kim, E.Y. (Department of Chemical Engineering, The University of Seoul) ;
  • Choi, Y.J. (Department of Urban Environment, Seoul Development Institute) ;
  • Chae, H.J. (Department of Food and Biotechnology and Department of Innovative Industrial Technology, Hoseo University) ;
  • Chu, K.H. (Department of Chemical and Process Engineering, University of Canterbury)
  • Published : 2006.10.30
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Abstract

An important issue in the oxidation of pentachlorophenol (PCP) by the enzyme horseradish peroxidase (HRP) is enzyme inactivation during the reaction. This study was initiated to investigate the ability of two nonionic surfactants (Tween 20 and Tween 80) to mitigate HRP inactivation. The surfactants were tested at concentrations below and above their critical micelle concentrations (CMCs). Enhancement of PCP oxidation was observed at sub-CMCs, indicating effective protection of HRP by the two surfactants. Maximum levels of PCP removal were observed when the concentrations of Tween 20 and Tween 80 were 40 and 50% of the CMCs, respectively At supra-CMCs, both surfactants caused a noticeable reduction in the extent of PCP removal.

Keywords

References

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