Biotechnology and Bioprocess Engineering:BBE

  • 제11권3호
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  • Pages.251-257
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  • 2006
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Optimization of ${\gamma}-Polyglutamic$ Acid Production by Bacillus subtilis ZJU-7 Using a Surface-response Methodology

  • Shi, Feng (Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University) ;
  • Xu, Zhinan (Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University) ;
  • Cen, Peilin (Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University)
  • 발행 : 2006.06.30
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초록

The components of the media used to elicit the biosynthesis of $poly-{\gamma}-glutamic$ acid $({\gamma}-PGA)$ by Bacillus subtilis ZJU-7 were investigated, particularly the carbon and nitrogen sources Of the 7 carbon sources investigated, sucrose induced the highest rate of ${\gamma}-PGA$ productivity; among the nitrogen sources, tryptone had the best effect for ${\gamma}-PGA$ production. A $2^{6-2}$ fractional factorial design was used to screen factors that influence ${\gamma}-PGA$ production significantly, and a central composite design was finally adopted to formulate the optimal medium. ${\gamma}-PGA$ productivity improved approximately 2-fold when the optimal medium was used compared with the original nonoptimized medium, and volumetric productivity reached a maximum of 58.2 g/L after a 24-h cultivation period.

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