Lactic Acid Fermentation of Chinese Yam (Dioscorea batatas Decne) Flour and Its Pharmacological Effect on Gastrointestinal Function in Rat Model

  • Shin, Kyung-Ok (Faculty of Food Industrial Technology, Catholic University of Daegu) ;
  • Jeon, Jeong-Ryae (College of Medicine, Yeungnam University) ;
  • Lee, Ji-Seon (Institute of Biotechnology, Yeungnam University) ;
  • Kim, Jong-Yeon (College of Medicine, Yeungnam University) ;
  • Lee, Chu-Hee (College of Medicine, Yeungnam University) ;
  • Kim, Soon-Dong (Faculty of Food Industrial Technology, Catholic University of Daegu) ;
  • Yu, Yeon-Su (Faculty of Pharmacy, Yeungnam University) ;
  • Nam, Doo-Hyun (Institute of Biotechnology, Yeungnam University)
  • 발행 : 2006.06.30

초록

To develop a health-aid preparation of Chinese yam (Dioscorea batatas Decne), lactic acid fermentation was attempted using a mixed starter comprising of Lactobacillus acidophilus, Streptococcus thermophilus, and Bifidobacterium bifidus. The anaerobic fermentation of a 5% Chinese yam flour suspension gave a uniform suspension of pH 4.35, containing $7.76{\times}10^6 CFU/mL$ lactic acid bacteria (LAB), and which was found to be acceptable to the panel from a sensory assessment. During the administration of the lactic acid fermented (LAF) Chinese yam to Sprague Dawley rats for 6 weeks, a smaller body weight gain, but greater excretion of feces were observed, implying the creation of a healthy gastrointestine on the administration of LAF Chinese yam, which was also confirmed by the gastrointestinal motility of the feed in rats fed on LAF Chinese yam. The constipation induced by loperamide was further suppressed in a rat group fed on a LAF Chinese yam diet, which was qualified from healthy gastrointestinal flora established by LAB. A serochemical analysis revealed a slight improvement in the blood glucose, neutral lipid and total cholesterol concentrations on administration of LAF Chinese yam, suggesting LAF Chinese yam could be served as a healthy-aid preparation, even for hyperglycemia or hyperlipidemia patients.

키워드

참고문헌

  1. Pharmacopoeia of the People's Republic of China (English Ed.) (1997) Vol. 1. Chemical Industry Press, Beijing, China
  2. Choi, E. M., S. J. Koo, and J.-K. Hwang (2004) Immune cell stimulating activity of mucopolysaccharide isolated from yam (Discorea batatas). J. Ethnopharmacol. 91: 1-6 https://doi.org/10.1016/j.jep.2003.11.006
  3. Zhao, G., J. Kan, Z. Li, and Z. Chen (2005) Structural features and immunological activity of a polysaccharide from Dioscorea opposita Thunb roots. Carbohydr. Polym. 61: 125-131 https://doi.org/10.1016/j.carbpol.2005.04.020
  4. Kim, M.-J., H.-N. Kim, K.-S. Kang, N.-I. Baek, D.-K. Kim, Y.-S. Kim, B.-H. Jeon, and S.-H. Kim (2004) Methanol extract of Dioscorea Rhizoma inhibits proinflammatory cytokines and mediators in the synoviocytes of rheumatoid arthritis. Int. Immunopharmacol. 4: 1489-1497 https://doi.org/10.1016/j.intimp.2004.07.001
  5. Hu, K. and X. Yao (2003) The cytotoxicity of methyl protodioscin against human cancer cell lines in vitro. Cancer Invest. 21: 389-393 https://doi.org/10.1081/CNV-120018230
  6. Hu, K. and X. Yao (2003) The cytotoxicity of methyl protoneogracillin (NSC-698793) and gracillin (NSC-698787), two steroidal saponins from the rhizomes of Dioscorea collettii var. hypoglauca, against human cancer cells in vitro. Phytother. Res. 17: 620-626 https://doi.org/10.1002/ptr.1211
  7. Yin, J., Y. Tezuka, K. Kouda, Q. L. Tran, T. Miyahara, Y. Chen, and S. Kadota (2004) Antiosteoporotic activity of the water extract of Dioscorea spongiosa. Biol. Pharm. Bull. 27: 583-586 https://doi.org/10.1248/bpb.27.583
  8. Hou, W.-C., H.-J. Chen, and Y.-H. Lin (1999) Dioscorins, the major tuber storage proteins of yam (Dioscorea batatas Decne), with dehydroascorbate reductase and monodehydroascorbate reductase activities. Plant Sci. 149: 151-156 https://doi.org/10.1016/S0168-9452(99)00152-1
  9. Hou, W.-C., M.-H. Lee, H.-J. Chen, W.-L. Liang, C.-H. Han, Y.-W. Liu, and Y.-H. Lin (2001) Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber. J. Agric. Food Chem. 49: 4956- 4960 https://doi.org/10.1021/jf010606m
  10. McAnuff, M. A., W. W. Harding, F. O. Omoruyi, H. Jacobs, E. Y. Morrison, and H. N. Asemota (2005) Hypoglycemic effects of steroidal sapogenins isolated from Jamaican bitter yam, Disocorea polygonoides. Food Chem. Toxicol. 43: 1667-1672 https://doi.org/10.1016/j.fct.2005.05.008
  11. Kwon, C.-S., H.-Y. Sohn, S.-H. Kim, J.-H. Kim, K.-H. Son, J.-S. Lee, J.-K. Lim, and J.-S. Kim (2003) Antiobesity effect of Dioscorea nipponica Makino with lipaseinhibitory activity in rodents. Biosci. Biotechnol. Biochem. 67: 1451-1456 https://doi.org/10.1271/bbb.67.1451
  12. Chen, H.-L., C.-H. Wang, C.-T. Chang, and T.-C. Wang (2003) Effects of Taiwanese yam (Dioscorea japonica Thunb var. pseudojaponica Yamamoto) on upper gut function and lipid metabolism in Balb/c mice. Nutrition 19: 646-651 https://doi.org/10.1016/S0899-9007(03)00058-3
  13. Jeon, J. R., J. S. Lee, C. H. Lee, J. Y. Kim, S. D. Kim, and D. H. Nam (2006) Effect of ethanol extract of dried Chinese yam (Dioscorea batatas) flour containing dioscin on gastrointestinal function in rat model. Arch. Pharm. Res. 29: 348-353 https://doi.org/10.1007/BF02968583
  14. Gilliland, S. E. (1990) Health and nutritional benefits from lactic acid bacteria. FEMS Microbiol. Rev. 7: 175-188
  15. Gorbach, S. L. (1990) Lactic acid bacteria and human health. Ann. Med. 22: 37-41 https://doi.org/10.3109/07853899009147239
  16. Carr, F. J., D. Chill, and N. Maida (2002) The lactic acid bacteria: a literature survey. Crit. Rev. Microbiol. 28: 281-370 https://doi.org/10.1080/1040-840291046759
  17. Yoo, E.-J., H.-S. Lim, K.-O. Park, and M. R. Choi (2005) Cytotoxic, antioxidative, and ACE inhibiting activities of Dolsan leaf mustard juice (DLMJ) treated with lactic acid bacteria. Biotechnol. Bioprocess Eng. 10: 60-66 https://doi.org/10.1007/BF02931184
  18. Lee, C.-H. (1997) Lactic acid fermented foods and their benefits in Asia. Food Control 8: 259-269 https://doi.org/10.1016/S0956-7135(97)00015-7
  19. Yoon, K. Y., E. E. Woodams, and Y. D. Hang (2004) Probiotication of tomato juice by lactic acid bacteria. J. Microbiol. 42: 315-318
  20. Lee, N. H., S. W. Oh, and Y. M. Kim (1996) Biochemical changes in muscle protein of squid sikhae during fermentation effects of temperature and moisture content. Kor. J. Food Sci. Technol. 28: 292-297
  21. Meilgaard, M., G. V. Civille, and B. T. Carr (1987) Sensory Evaluation Techniques. pp. 39-112. CRC Press, Inc., Boca Raton, Florida, USA
  22. Aderiye, B. I. and A. A. Ogunjobi (1998) Fermentation of yam: microbiology and sensory evaluation of cooked fermented yam tissues. Plant Foods Hum. Nutr. 52: 49-54 https://doi.org/10.1023/A:1008023603890