Biotechnology and Bioprocess Engineering:BBE

  • 제11권3호
  • /
  • Pages.223-229
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  • 2006
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Kinetics and Modelling of Cell Growth and Substrate Uptake in Centella asiatica Cell Culture

  • Omar, Rozita (Department of Chemical and Environmental Engineeriing, Faculty of Engineeriing, Universiti Putra Malaysia) ;
  • Abdullah, M.A. (Department of Chemical Engineering, Universiti Teknologi Petronas) ;
  • Hasan, M.A. (Department of Bioprocess Techology, Universiti Putra Malaysia) ;
  • Rosfarizan, M. (Department of Bioprocess Techology, Universiti Putra Malaysia) ;
  • Marziah, M. (Department of Biochemistry, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia)
  • 발행 : 2006.06.30
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초록

In this study, we have conducted kinetics and modelling studies of Centella asiatica cell growth and substrate uptake, in an attempt to evaluate cell growth for a better understanding and control of the process. In our bioreactor cultivation experiment, we observed a growth rate of 0.18/day, a value only 20% higher than was seen in the shake flask cultivation trial. However, the observed maximum cell dry weight in the shake flask, 10.5g/L, was 14% higher than was achieved in the bioreactor. Ninety seven percentage confidence was achieved via the fitting of three unstructured growth models; the Monod, Logistic, and Gompertz equations, to the cell growth data. The Monod equation adequately described cell growth in both cultures. The specific growth rate, however, was not effectively predicted with the Logistic and Gompertz equations, which resulted in deviations of up to 73 and 393%, respectively. These deviations in the Logistic and Gompertz models may be attributable to the fact that these models were developed for substrate-independent growth and fungi growth, respectively.

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