Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Production of Hepatitis B Core Antigen in a Stirred Tank Bioreactor: The Influence of Temperature and Agitation

  • Tey, Beng Ti (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia) ;
  • Chua, Mung Ing (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia) ;
  • Chua, Ghee Sung (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia) ;
  • Ng, Michelle Yeen Tan (Institute of Bioscience, University Putra Malaysia) ;
  • Biak, Dayang Radiah Awang (Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra Malaysia) ;
  • Tan, Wen Siang (Institute of Bioscience, University Putra Malaysia) ;
  • Ling, Tau Chuan (Institute of Bioscience, University Putra Malaysia)
  • Published : 2006.04.30
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Abstract

The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli$(0.844 h^{-1})$ was achieved at a temperature of $37^{\circ}C$ and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W3110lQ in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of $44^{\circ}C$ and an agitation speed of 250 rpm. The relative protein concentration at $44^{\circ}C$ is 30 and 6% higher compared to that at 30 and $37^{\circ}C$, respectively.

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