농약과학회지 (The Korean Journal of Pesticide Science)

  • 제10권2호
  • /
  • Pages.107-116
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  • 2006
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지

한국산 곤충병원성 선층의 녹색콩풍뎅이(Popillia quadriguttata), 주황긴다리풍뎅이(Ectinohoplia rufipes), 연다색풍뎅이(Phyllopertha diversa) 유충에 대한 병원성과 골프장에서 지속성

Pathogenicity of Korean Entomopathogenic Nematodes on Larva of Popillia quadriguttata, Ectinohoplia rufipes and Phyllopertha diversa (Coleoptera: Rutelidae) and Persistence in Golf Courses

  • 최우근 (서원벨리골프장) ;
  • 하판정 ((주)세실) ;
  • 이상명 (국립산림과학원 남부산림연구소) ;
  • 추호렬 (경상대학교 응용생물환경학과, 농업생명과학연구원) ;
  • 이동운 (상주대학교 생물응용학과)
  • Choi, Woo-Geun (Seowon Valley Golf Club) ;
  • Ha, Pan-Jung (SESIL Cooperation) ;
  • Lee, Sang-Myeong (Southern Forest Research Center, National Forest Science Institute) ;
  • Choo, Ho-Yul (Department of Applied Biology and Environment Sciences, Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Lee, Dong-Woon (Department of Applied Biology, Sangju National University)
  • 발행 : 2006.06.30
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초록

한국산 곤충병원성 선충의 녹색콩풍뎅이(Popillia quadriguttata), 주황긴다리풍뎅이(Ectinohoplia rufipes) 및 연다색풍뎅이(Phyllopertha diversa)에 대한 병원성을 실내에서 검정하였으며 아울러 Heterorhabditis sp. KCTC 0991BP 계통을 이용하여 굼벵이 방제효과와 지속성을 조사하였다. 실내실험 결과 녹색콩풍뎅이 3령충에 대하여 H. bacteriophora Hamyang 계통은 95%의 높은 치사율을 보였으며 주황긴다리풍뎅이 3령충에 대해서도 H. bacteriophora Hamyang 계통과 Heterorhabditis sp. KCTC 0991BP 계통이 80% 이상의 치사율을 나타내었다. 그러나 연다색풍뎅이는 병원성이 가장 높은 Heterorhabditis sp. KCTC 0991BP 계통 처리 시에도 60%의 치사율을 보였다. 골프장에서 Heterorhabditis sp. KCTC 0991BP 계통을 $2.24{\times}10^9$ Ijs $ha^{-1}$ 농도로 처리하였을 때 굼벵이의 밀도는 39%까지 감소되었고, 골프장 페어웨이에서 지속성은 $5{\sim}10$ cm 깊이의 토양에서 두 달까지 지속되었으나 $0{\sim}5$ cm 깊이에서는 치사되는 미끼곤충(꿀벌부채명나방)이 없어 지속성이 낮았다.

Korean entomopathogenic nematode strain were evaluated against white grubs, Popillia quadriguttata, Ectinohoplia rufipes and Phyllopertha diversa in laboratory. In addition we examined the efficacy of entomopatogenic nematode, Heterorhabditis sp. KCTC 0991BP strain against white grubs in Seaside Golf Club in Gimpo, Gyeonggii and persistence of entomopatogenic nematode in fairway of Anyang Benest Golf Club in Gunpo, Gyeonggii. In laboratory Heterorhabditis bacteriophora Hamyang strain showed 95% mortality against Popillia quadriguttata. H. bacteriophora Hamyang strain and Heterorhabditis sp. KCTC 0991BP induced 80% mortality against 3rd instar of Ectinohoplia rufipes. However, the letter showed only 60% mortality against Phyllopertha diversa. White grub density was reduced to 39% by the application of Heterorhabditis sp. KCTC 0991BP at the rate of $2.24{\times}10^9$ infective juveniles $ha^{-1}$ compared with control in Seaside Golf Club. Heterorhabditis sp. KCTC 0991BP persisted for two months at $5{\sim}10$ cm soil depth but not persisted at $0{\sim}5$ cm soil depth for the same periods when applied at the rate of $2.47{\times}10^9$ infective juveniles $ha^{-1}$.

키워드

참고문헌

  1. Alm, S. R., T. Yeh, J. L. Hanula and R. Gaorgis (1992) Biological control of Japanese, oriental, and black turfgrass atenius beetle (Coleoptera: Scarabaeidae) larvae with entomopathogenic nematodes (Nematoda: Steinernematidae, Heterorhabditidae). J. Econ. Entomol. 85:1660-1665 https://doi.org/10.1093/jee/85.5.1660
  2. Blackshaw, R. P. (1988) A survey of insect parasitic nematodes in Northern Ireland Ann. Appl. BioI. 113:561-565 https://doi.org/10.1111/j.1744-7348.1988.tb03333.x
  3. Campbell, J. F., G. Orza, F. Yoder, E. Lewis and R. Gaugler (1998) Spacial and temporal distribution of endemic and released entomopathogenic nematode populations in turfgrass. Entomologia Experimentalis et Applicata 86: 1-11 https://doi.org/10.1023/A:1003130111093
  4. Choo, H. Y., D. W. Lee, J. W. Park, H. K. Kaya, D. R Smitly, S. M. Lee and Y. M. Choo (2002) Life history and spatial distribution of oriental beetle (Coleoptera: Scarabaeidae) in golf courses in Korea. J. Econ. Entomol. 95:72-80 https://doi.org/10.1603/0022-0493-95.1.72
  5. Choo, H. Y., H. K. Kaya and S. P. Stock. 1995. Isolation of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Korea. Japanese J. Nematol. 25:44-51 https://doi.org/10.3725/jjn1993.25.1_44
  6. Converse, V. and P. S. Grewal (1998) Virulence of entomopathogenic nematodes to the Wastern masked chafer Cyclocephala hirta (Coleoptera: Scarabaeidae). J. Econ. Entomol. 91:428-432 https://doi.org/10.1093/jee/91.2.428
  7. Dutky, S. R., J. V. Thompson and G. E. Cantwell (1964) A technique for the mass production of the DD-136 nematode. J. Insect Pathol. 6:417-422
  8. Glazer, I. and A GoI'berg (1993) Field efficacy of entomopathogenic nematodes against the beetle Maladera matrida (Coleoptera: Scarabaeidae). Biocontrol Science and Technology 3:367-376 https://doi.org/10.1080/09583159309355291
  9. Grewal, P. S., E. E. Lewis, R. Gaugler and J. F. Campbell (1994) Host-finding behaviour as a predictor of foraging strategy in entomopathogenic nematodes. Parasitol. 108:207-215 https://doi.org/10.1017/S003118200006830X
  10. Kaya, H. K. (1990) Soil ecology. pp.93-115, In Entomopathogenic nematodes in biological control (eds. R. Gaugler and H. K. Kaya), CRC Press, U.S.A
  11. Kaya, H. K. and R. Gaugler (1993) Entomopathogenic nematodes. Annu. Rev. Entomol. 38:181-206 https://doi.org/10.1146/annurev.en.38.010193.001145
  12. Klein. M. G. (1990) Efficacy against soil-inhibiting insect pests. pp. 195-214, In Entomopathogenic nematodes in biological control (eds. R. Gaugler and H. K. Kaya), CRC Press, U.S.A
  13. Koppenofer, A.M., H. K. Kaya and S. P. Taormino (1995) Infectivity of entomopathogenic nematodes (Rhabditida: Steinernematidae) at different soil depths and moistures. J. Invertebr. Pathol. 65:193-199 https://doi.org/10.1006/jipa.1995.1028
  14. Koppenofer, A. M., H. Y. Choo, H. K. Kaya, D. W. Lee and W. D. Gelernter (1999) Increased field and greenhouse efficacy against scarab grubs with a combination of an entomopathogenic nematode and Bacillus thuringiensis. Biological Control 14: 37-44 https://doi.org/10.1006/bcon.1998.0663
  15. Lee, D. W., H. Y. Choo, H. K. Kaya, S. M. Lee, D. R. Smitly, S. K. Shin and C. G. Park (2002) Laboratory and field evaluation of Korean entomopathogenic nematode isolates against the oriental beetle, Exomala orientalis (Coleoptera: Scarabaeidae). J. Econ. Entomol. 95:918-926 https://doi.org/10.1603/0022-0493-95.5.918
  16. Lossbroek, T. G. and J. Theunissen (1985) The entomopathogenous nematode Neoaplectana bibionis as a biological control agent of Agrotis segetum in lettuce. Entomolo. Exp. Appl. 39:261-264 https://doi.org/10.1111/j.1570-7458.1985.tb00468.x
  17. Molyneux, A. S. and R. A. Bedding (1984) Influence of soil texture and moisture on the infectivity of Heterorhabditis sp. D1 and Steinernema glaseri for larvae of the sweep blowfly, Lucilia cuprina. Nematologica 30:358-365 https://doi.org/10.1163/187529284X00266
  18. Potter, D. A (1998) Destructive turfgrass insects: biology, diagnosis, and control. Ann Arbor Press. p.344. U.S.A
  19. Selvan, S., P. S. Grewal, R. Gaugler and M. Tomalak (1994) Evaluation of Steinemematid nematodes against Popillia japonica (Coleoptera: Scarabaeidae) larvae: species, strain, and rinse after application. J. Econ. Entomol. 87:605-609 https://doi.org/10.1093/jee/87.3.605
  20. Selvan, S., R. Gaugler and J. F. Campbell (1993) Efficacy of entomopathogenic strains against Popillia japonica (Coleoptera: Scarabaeidae) larvae. J. Econ. Entomol. 86:353-359 https://doi.org/10.1093/jee/86.2.353
  21. Sulistyanto, D. and R-U. Ehlers (1996) Efficacy of the entomopathogenic nematodes Heterorhabditis megidis and Heterorhabditis bacteriophora for the control of grubs (Phyllopertha horticola and Aphodius contaminatus) in golf course turf. Biocontrol Sci. and Tech. 6:247-250 https://doi.org/10.1080/09583159650039430
  22. Wang, Y., J. F. Campbell and R. Gaugler (1995) Infection of entomopathogenic nematodes Steinernema glaseri and Heterorhabditis bacteriophora against Popillia japonica (Coleoptera: Scarabaeidae) larvae. J. Invertebr. Pathol. 66: 178-184 https://doi.org/10.1006/jipa.1995.1081
  23. Wang, Y., R. Gaugler and L. Cui (1994) Variations in immune response of Popillia japonica and Acheta domesticus to Heterorhabditis bacteriophora and Steinernema species. J. Nematol. 26:11-18
  24. Woodring, J. L. and H. K. Kaya (1988) Steinernematidae and heterorhabditid nematodes: a handbook of techniques. Southern Coop. Ser. Bull. 331, Alkansas Agri. Exp. Sm. Fayetteville, p.29. U.S.A
  25. Wright, G. J., M. G. Villani and F. Agudelo-silva (1988) Steinernematid and heterorhabditid nematodes for control of larval european chafers and Japanese beetles (Coleoptera: Scarabaeidae) in potted yew. J. Econ. Entomol. 81:152-157 https://doi.org/10.1093/jee/81.1.152
  26. Yeh, T. and S. R. Alm (1992) Effects of entomopathogenic nematode species, rate, soil moisture, and bacteria on control of Japanese beetle (Coleoptera: Scarabaeidae) larvae in the laboratory. J. Econ. Entomol. 85:2144-2148 https://doi.org/10.1093/jee/85.6.2144
  27. 김형기 (1991) 잔디학. 선진문화사. 545p
  28. 이동운, 김형환, 이상명, 추호렬, 최우근, 권태웅 (2005) 등얼룩풍뎅이(Exomala orientalis)에 대한 한국산 곤충병원성 선충의 병원성. 한국잔디학회지 19:39-46
  29. 이동운, 추호렬, 신옥진, 윤재수, 김영섭 (2002a) 주둥무늬차색풍뎅이(Adoretus tenuimaculatus)에 의한 퍼레니얼라이그라스(Lolium prenne) 피해와 한국산 곤충병원성 선충을 이용한 생물적 방제. 한응곤지. 41:217-223
  30. 이동운, 추호렬, 정재민, 이상명, 사공영보 (2002b) 녹색콩풍뎅이(Popillia quadrguttata)의 기주식물. 한응곤지. 41:15-19
  31. 조인호 (1996) SAS의 이용과 실제. 성안당. 665p
  32. 최우근, 이동운, 추호렬, 정재민, 이상명, 박정규 (2001) 주황긴다리풍뎅이 (Ectinohoplia rufipes)의 골프장 기주식물과 피해 잎의 성충 유인 효과. 한응곤지. 40:31-40
  33. 추호렬, 이동운, 박지웅, 이종원 (1999) 골프장 발생주요 풍뎅이 4종, 주황긴다리풍뎅이, 주둥무늬차색풍뎅이, 등얼룩풍뎅이, 녹색콩풍뎅이의 비교. 한국잔디학회지 13:101-112
  34. 추호렬, 이동운, 이상명, 권태웅, 성영탁, 조팔용 (1998) 골프장 코스 내 잔디 가해 굼벵이 종류와 계절별 밀도. 한국잔디학회지 12:225-236
  35. 추호렬, 이동운, 이상명, 이태우, 최우근, 정영기, 성영탁 (2000) 골프장 잔디해충과 천적의 종류. 한웅곤지 39:171-179