Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

THE EFFECT OF PRP ON THE BONE REGENERATION OF ${\beta}-TCP$ GRAFTED IN RABBIT CRANIAL BONE DEFECT

가토 두개골 결손부에 이식된 ${\beta}-TCP$의 골치유 과정에서 PRP의 효과에 관한 연구

  • Lee, Soung-Hoon (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Hwang, Kyung-Gyun (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Park, Chang-Joo (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Lim, Byung-Sup (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Cho, Jung-Yeon (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Paik, Seung-Sam (Department of Pathology, College of Medicine, Hanyang University) ;
  • Shim, Kwang-Sup (Department of Oral & Maxillofacial Surgery, College of Medicine, Hanyang University)
  • 이성훈 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 황경균 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 박창주 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 임병섭 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 조정연 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 백승삼 (한양대학교 의과대학 병리학교실) ;
  • 심광섭 (한양대학교 의과대학 치과학교실 구강악안면외과)
  • Published : 2006.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose : Platelet rich plasma (PRP) is an autologous material with many growth factors, such as BMPs, PDGF, $TGF-{\beta}_1$, $TGF-{\beta}_2$, VEGF, and IGF, facilitating bone healing process. The prominent osteoconductive activity and the biodegradable nature of beta-tricalciumphosphate (${\beta}-TCP$) for bone grafts in animal experiments have been reported. The purpose of this study was to evaluate the effect of PRP on the osteogenesis of ${\beta}-TCP$. Materials & Methods : Two artificial calvarial bone defects were made in 32 rabbits which were divided into 2 groups. In one group of 16 rabbits, autogenous bone / ${\beta}-TCP$ was grafted on each side of cranial bone defect. In the other group of 16 rabbits, mixture of ${\beta}-TCP$ and PRP / PRP alone was grafted on each side of the cranial bone defect. The animals were sacrificed at 2, 4, 8, and 12 weeks after surgery. The specimens were harvested and examined histologically and immunohistochemically by the expression of BMP2/4/7, PDGF, VEGF and $TGF-{\beta}_1$. Results : The mean volume of new bone formation was significantly higher at 4, 8, 12 weeks in autogenous graft than that in ${\beta}-TCP$. The BMP2/4 expression was significantly higher at 4 weeks in autogenous bone graft and at 4 weeks in mixture of ${\beta}-TCP$ and PRP and at 12 weeks in ${\beta}-TCP$. The expression of BMP7, PDGF, VEGF and $TGF-{\beta}_1$ showed no significant difference in autogenous, ${\beta}-TCP$, mixture of ${\beta}-TCP$ and PRP, and PRP alone during grafted bone regeneration. Conclusion : The results showed that PRP had no additional value in promoting healing process of ${\beta}-TCP$ grafts.

Keywords

References

  1. Merkx MA, Maltha JC, Stoelinga PJ : Assessment of value of anorganic bone additives in sinus floor augmentation : a review of clinical reports. Int J Oral Maxillofac Surg 32 : 1, 2003 https://doi.org/10.1054/ijom.2002.0346
  2. Laurencin CT : Bone Graft Substitutes, 1st ed. Philadelphia, International Co, 2003, p.180
  3. Bahn S : Plaster: A Bone substitute. Oral Surg Oral Med Oral Pathol 21 : 672, 1966 https://doi.org/10.1016/0030-4220(66)90045-4
  4. Saito M, Shimizu H, Beppu M et al : The role of ${\beta}-Tricalcium$ Phosphate in vascularized periosteum. J Orthop Sci 5 : 275, 2000 https://doi.org/10.1007/s007760050163
  5. Ogose A, Hotta T, Hatano H et al : Histological examination of ${\beta}-TCP$ graft in human femur. J Biomed Mater Res 63 : 601, 2002 https://doi.org/10.1002/jbm.10380
  6. Tayapongsak PO, Brien DA, Monterio CB et al : Autologous fibrin adhesive in mandibular reconstruction with particulate cancellous bone marrow. J Oral Maxillofac Surg 52 : 161,1994 https://doi.org/10.1016/0278-2391(94)90401-4
  7. Marx RE : Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85 : 638, 1998 https://doi.org/10.1016/S1079-2104(98)90029-4
  8. Landesberg R, Roy M, Glickman RS : Quantification of growth factor levels using a simplified method of plateletrich plasma gel preparation. J Oral Maxillofac Surg 58 : 297, 2000 https://doi.org/10.1016/S0278-2391(00)90058-2
  9. Miyazono K, Ten-Dijke P, Ichiyo H et al : Reports for transforming growth facter betar. Adx Immunol 55 : 181, 1994
  10. Canalis E, Centrella M, Busch W et al : Insuline like growth factor I mediates selective anabolic effects of parathyroid hormone in bone culture. J Clin Invest 83 : 60, 1989 https://doi.org/10.1172/JCI113885
  11. Ducheyne P, Qiu Q : Bioactive ceramics : The effect of surface reactivity on bone formation and bone cell function. Biomaterials 20 : 2287, 1999 https://doi.org/10.1016/S0142-9612(99)00181-7
  12. Lu J, Descamps M, Dejou J et al : The Biodegradation Mechanism of Calcium Phosphate Biomaterials in Bone. J Biomed Mater 63 : 408, 2002 https://doi.org/10.1002/jbm.10259
  13. Merten HA, Wiltfang J, Grohmann U et al : Intraindividual Comparative Animal Study of ${\alpha}-and\;{\beta}-Tricalcium$ Phosphate Degradation in Conjunction with Simultaneous Insertion of Dental Implants. J Craniofac Surg 12 : 59, 2001 https://doi.org/10.1097/00001665-200101000-00010
  14. Szabo G, Suba Z, Hrabak K et al : Autogenous Bone Versus ${\beta}-Tricalcium$ Phosphate Graft Alone for Bilateral Sinus Elevations (2-and 3-Dimensional Computed Tomographic, Histologic, and Histomorphometric Evaluations): Preliminary Results. Int J Oral Maxillofac Implants 16 : 681, 2001
  15. Lee SH, Song SI, Hwang KG et al : The experimental study of the bone regeneration on ${\beta}-TCP$ in rabbit cranial bone. J Kor Oral Maxillofac Surg 30 : 282, 2004
  16. Yamada Y, Ueda M, Naiki T et al : Tissue-engineered injectable bone regeneration for osseointegrated dental implants. Clin Oral Imlants Res 15 : 589, 2004 https://doi.org/10.1111/j.1600-0501.2004.01038.x
  17. Vanassche B, Defranq J : Use of PRP in bone volume augmentation. Revue Belge de Medicine Dentaire 56 : 125, 2001
  18. Gruber R, Vargga F, Fischer MB et al : Platelets stimulate proliferration of bone cells : involvement of platelet derived growth factor, microparticles and membranes. Clin Oral Implants Res 31 : 529, 2002
  19. Aghaloo TL, Moy PK, Freymiller EG : Investigation of platelet-rich plasma in rabbit cranial defects : a pilot study. J Oral Maxillofac Surg 60 : 1176, 2002 https://doi.org/10.1053/joms.2002.34994
  20. Jakse N, Tangle S, Gili R et al : Influence of PRP on autologous sinus grafts. An experimental study on sheep. Clin Oral Implants Res 14 : 578, 2003 https://doi.org/10.1034/j.1600-0501.2003.00928.x
  21. Kassolis J, Rosen PS, Reynolds MA : Alveolar ridge and sinus augmentation utilizing platelet-rich plasma in combination with freeze-dried bone allograft : case series. J Periodontol 71 : 1654, 2000 https://doi.org/10.1902/jop.2000.71.10.1654
  22. Furst G, Gruber R, Tangl S et al : Sinus grafting with autogenous platelet-rich plasma and bovine hydroxyapatite. Clin Oral Implants Res 31 : 529, 2003
  23. Fennis JPM, Stoelinga PJW et al : Mandibular reconstruction : a clinical and radiografic animal study on the use of autogenous scaffolds and platelet-rich plasma. Int J Oral Maxillofac Surg 31 : 281, 2002 https://doi.org/10.1054/ijom.2002.0151
  24. Suba Z, Takacs D, Gyulai-Gaal S et al : Facilitation of ${\beta}-Tricalcium$ Phosphate-Induced Alveolar Bone Regeneration by platelet-rich plasma in Beagle Dogs: A Histologic and Histomorphometric Study. Int J Oral Maxillofac Implants 6 : 832, 2004
  25. Raghoebar GM, Timmenga NM, Reintsema H et al : Maxillary bone grafting for the insertion of endosseous implants : result after 12-124 months. : Clinl Oral Implants Res 12 : 279, 2001 https://doi.org/10.1034/j.1600-0501.2001.012003279.x
  26. Froum SJ, Wallace SS, Tarnow DP et al : Effect of platelet-rich plasma on bone growth and osseointegration in human maxillary sinus grafts : three bilateral case reports. Int J Periodontics Restorative Dent 22 : 45, 2002
  27. Urist MR, Raskin K, Goltz D et al : Endogenous bone morphogenic protein : immunohistochemical localization in repair of a punch hole in the rabbit's ear. Plast Reconstr Surg 99 : 1382, 1997 https://doi.org/10.1097/00006534-199704001-00028
  28. Bolander MR : Regeneration of fracture repair by growth factors. Proc Soc Exp Bio Med 2000 : 165, 1992
  29. Taylor JC, Cuff SE, Leger JP et al : In vitro osteoclast resorption of bone substitute biomaterials used for implant site augmentation : A pilot study. Int J Oral Maxillofac Implants 17 : 321, 2002
  30. Flautre B, Maynou C, Lemaitre J et al : Bone colonization of ${\beta}-TCP$ granules incorporated in brushite cements. J Biomed Mater Res 63 : 413, 2002 https://doi.org/10.1002/jbm.10262
  31. Samuel E, Lynch SE, Robert J et al : Tissue Engineering Applications in Maxillofacial Surgery and Periodontics. 1st ed. Illinois, Quintessence Publishing Co, 1999, p.73
  32. Stenport VF, Johansson C, Heo SJ et al : Titanium implants and BMP-7 in bone: an experimental model in the rabbit. J Mater Sci Mater Med 14 : 247, 2003 https://doi.org/10.1023/A:1022884724059
  33. Trisi P, Rao W, Rebaudi A et al : Histologic effect of purephase beta-tricalcium phosphate on bone regeneration in human artificial jaw bone defects. Int J Periodontics Restorative Dent 23 : 69, 2003
  34. Aldecoa EA, Ortiz IA : A new approach to bone regeneration. 1st ed. New York, Puesta Al Dia Publicaciones, 2002, p.88
  35. Street J, Bao M, de Guzman L et al : Vascular endotherial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. PNAS 99 : 9656, 2002 https://doi.org/10.1073/pnas.152324099