Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Glutathione (GSH) Response as a Metabolic Biomarker to Benzo(α)pyrene and Aroclor 1254 Exposure in the Pacific Oyster Crassostrea gigas

  • Jo, Qtae (East Sea Fisheries Research Institute) ;
  • Choy, Eun-Jung (Department of Biology, Pusan National University) ;
  • Lee, Yong-Hwa (East Sea Fisheries Research Institute) ;
  • Ko, Sung-Jung (Korea Institute of Maritime and Fisheries Technology) ;
  • Song, Young-Chae (Department of Civil and Environmental Engineering, Korea Maritime University) ;
  • Kim, Myoung-Jin (Department of Civil and Environmental Engineering, Korea Maritime University) ;
  • Hwang, Eung-Ju (Department of Environmental Engineering, Daegu University)
  • Published : 2006.12.30
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Abstract

We measured activities of the ubiquitous tripeptide non-protein thiol (L-${\gamma}$-glutamyl-L-cysteinyl-glycine), glutathione (GSH), which is believed to playa fundamental role in detoxifying xenobiotics in biological systems, as a metabolic biomarker for benzo(${\alpha}$)pyrene and Aroclor 1254 exposure in the Pacific oyster Crassostrea gigas. Reproductive oysters were exposed to the pollutants for 50 days by the algal vectoring method in which the oysters were fed with concentrated standard algal foods grown in culture media containing Aroclor 1254 (0, 5, 50, 500 ng/g) or benzo(${\alpha}$)pyrene (0, 10, 100, 1,000 ng/g). Both pollutants induced maternal GSH activities in 10 days in a dosage-dependent manner (p<0.05), although Aroclor 1254 was stronger. The pollutant-driven GSH elevation persisted for 20 to 30 days depending on the pollutants and concentrations. Thereafter, a drastic decline in the GSH activity was observed due to metabolic failure, after which the oyster GSH remained at low levels throughout the remainder of the experiment. The pollutant exposures influenced maternal reproductive output in terms of fertilization, hatching, and morphology. These results imply that changes in activity of the GST-catalyzing molecule can be used as an oyster biomarker for Aroclor 1254 and benzo(${\alpha}$)pyrene exposure.

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References

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