Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Optimization of Parameters for GUS Gene Transformation of Porphyra yezoensis by Particle Bombardment

  • Nam, Bo-Hye (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Park, Jung-Youn (Biotechnology Research Center, National Fisheries Research & Development Institute) ;
  • Jin, Deuk-Hee (Faculty of Marine Bioscience and Technology, Kangnung National University) ;
  • Hong, Yong-Ki (Department of Biotechnology, Pukyong National University)
  • Published : 2006.12.30
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Abstract

We optimized the biological and physical parameters for DNA delivery into thalli of the red alga Porphyra yezoensis using a particle bombardment device. The efficiency of transformation was determined using the ${\beta}-glucuronidase$ (GUS) assay. The optimal helium pressure, distance of tungsten particle flight, and ratio of DNA to tungsten particles were $23kgf/cm^2$, 8 cm, and $5{\mu}g/mg$ tungsten, respectively. During bombardment, osmotic treatment with a mixture of 0.6 M mannitol and sorbitol increased the efficiency of GUS transformation. After 2 days, the blue color indicating GUS activity was observed using a histochemical assay.

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References

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