Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Simultaneous Determination of Amoxicillin and Ampicillin in Fish Meat Using High-Performance Liquid Chromatography

HPLC를 이용한 어류 중의 Amoxicillin과 Ampicillin 항생제 동시 분석법

  • Jo, Mi-Ra (Food Sanitation Research Team, National Fisheries Research and Development Institute) ;
  • Kim, Poog-Ho (Food Sanitation Research Team, National Fisheries Research and Development Institute) ;
  • Lee, Tae-Seek (Food Sanitation Research Team, National Fisheries Research and Development Institute) ;
  • Oh, Eun-Gyoung (Food Sanitation Research Team, National Fisheries Research and Development Institute) ;
  • Yu, Hong-Sik (Food Sanitation Research Team, National Fisheries Research and Development Institute) ;
  • Lee, Hee-Jung (Food Sanitation Research Team, National Fisheries Research and Development Institute)
  • 조미라 (국립수산과학원 식품위생팀) ;
  • 김풍호 (국립수산과학원 식품위생팀) ;
  • 이태식 (국립수산과학원 식품위생팀) ;
  • 오은경 (국립수산과학원 식품위생팀) ;
  • 유홍식 (국립수산과학원 식품위생팀) ;
  • 이희정 (국립수산과학원 식품위생팀)
  • Published : 2006.12.31
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Abstract

A simultaneous high-performance liquid chromatography assay method for amoxicillin and ampicillin in fish products was developed, evaluated, and validated by monitoring these antibiotics in fish samples obtained from aquaculture and distribution. The recovery rate of this method was higher than those of conventional methods and was 95.3-106.6% for amoxicillin and 81.4-92.4% for ampicillin. Our pretreatment procedure sufficiently removed or reduced materials affecting HPLC analysis, such as low-molecular-weight substances. The performance limit of this method was evaluated as 0.01 ppm of amoxicillin and ampicillin in fish muscle. Finally, 171 fish samples, including olive flounder (Paralichthys olivaceus), common sea bass (Lateolabrax japonicus), and black rock fish (Sebastes schlegeli) collected from fish farms in the coastal area between April and September 2005 were analyzed to evaluate the overall efficiency of the method and to monitor the actual of amoxicillin and ampicillin usage in fish farms. The results indicated that the developed method was suitable for analyzing amoxicillin and ampicillin in fish muscle, and determined that those antibiotics were being used for fish farming but were not detected in fish samples during the shipping and distribution stages.

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References

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