한국가금학회지 (Korean Journal of Poultry Science)

  • 제33권4호
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  • Pages.303-308
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  • 2006
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지

산란계에 갯벌 미생물 유래 단백질 분해 효소제의 급여가 계란 품질, 영양소 소화율 및 혈청내 총 단백질 함량에 미치는 영향

Effects of Mud Flat Bacteria Origin Protease Supplementation on Egg Quality, Nutrient Digestibility and Total Protein Concentration of Serum in Laying Hens

  • Kim, H.J. (Department of Animal Resource & Science, Dankok University) ;
  • Cho, J.H. (Department of Animal Resource & Science, Dankok University) ;
  • Chen, Y.J. (Department of Animal Resource & Science, Dankok University) ;
  • Yoo, J.S. (Department of Animal Resource & Science, Dankok University) ;
  • Min, B.J. (Department of Animal Resource & Science, Dankok University) ;
  • Park, B.C. (CJ Feed Co. Ltd.) ;
  • Kim, I.H. (Department of Animal Resource & Science, Dankok University)
  • 발행 : 2006.12.30
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초록

본 연구는 산란계 사료내 갯벌 미생물 유래 단백질 분해 효소제를 첨가하였을 때 계란 품질, 영양소 소화율 및 혈청 내 총 단백질 함량에 미치는 영향을 알아보기 위해 실시하였다. 사양 시험은 29주령 ISA brown 갈색계 252수를 공시하며 8주간 실시하였다. 처리구는 옥수수-대두박 위주의 사료인 기초 사료구(CON; basal diet), 기초 사료내 단백질 분해 효소제 0.05% 첨가구(PROI; basal diet+0.05% pretense), 0.1% 첨가구 (PRO2; basal diet+0.1% protease)로서 처리구당 7반복, 반복당 12수씩 완전임의 배치하였다. 전체 사양시험 기간 동안, 산란율은 처리구간에 유의적인 차이를 보이지 않았다(P>0.05). 난황고의 변화량은 PRO1 처리구가 CON 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난중의 변화량은 PRO2처리구가 CON과 PRO1 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 난각 품질, 난황색, haugh unit 및 난황 계수에서는 처리구간에 유의적인 차이를 보이지 않았다(P>0.05). 건물 소화율에서는 CON과 PRO2처리구가 PRO1 처리구와 비교하여 유의적으로 높게 나타났고(P<0.05), 질소 소화율에서는 PRO2 처리구가 CON 처리구와 비교하여 유의적으로 높게 나타났다(P<0.05). 혈청내 총 단백질 함량에서는 처리구간에 유의적인 차이를 보이지 않았다(P>0.05). 결론적으로, 산란계 사료내 갯벌 미생물 유래 단백질 분해 효소제의 첨가는 난황고, 난중 및 영양소 소화율을 개선시켰다.

This study was conducted to investigate the effects of mud flat bacteria origin pretense supplementation on e99 qualify, nutrient digestibility and total protein concentration of serum in laying hens. A total of two hundred fifty two laying hens were randomly allocated into three treatments with seven replications for eight weeks. Dietary treatments included 1) CON (basal diet), 2) PRO1 (basal diet + 0.05% pretense) and 3) PRO2 (basal diet + 0.1% pretense). During the entire experimental peripd, hen-day egg production was not affected by treatments (P>0.05). Difference of yolk height was increased in PRO1 treatment compared with CON treatment (P<0.05). Difference of egg weight was increased in PRO2 treatment compared with CON and PRO1 treatments (P<0.05). Shell quality, yolk color unit, haugh unit and egg yolk index were not affected by treatments (P>0.05). DM digestibility was improved in CON and PRO2 treatments compared with PRO1 treatment (P<0.05). N digestibility was improved in PRO2 treatment compared with CON treatment (P<0.05). Total protein concentration in serum were not affected by treatments (P>0.05). In conclusion, mud flat bacteria origin pretense was effective for improving egg weight, yolk height and nutrient digestibility in laying hens.

키워드

참고문헌

  1. AOAC 1994 Official Methods of Analysis(16th Ed.). Association of official Analytical Chemists. Washington DC
  2. Benabdeljelil K, Arbaoui MI 1994 Effects of enzyme supplementation of barley-based diets on hen performance and egg quality. Anim Feed Sci Tec 48:325-334 https://doi.org/10.1016/0377-8401(94)90181-3
  3. Berg LR 1959 Enzyme supplementation of barley diets for laying hens. Poultry Sci 38:1132-1139 https://doi.org/10.3382/ps.0381132
  4. Berg LR 1961 Effect of adding enzyme to barley diets at different ages of pullets on laying house performance. Poultry Sci 40:34-39 https://doi.org/10.3382/ps.0400034
  5. Brenes A, Guenter W, Marquardt RR, Rotter BA 1993 Effect of ${\beta}$-glucanase/pentosanase supplementation on the performance of chickens and laying hens fed wheat, barley, naked oats and rye diet. Can J Anim Sci 73:941-951 https://doi.org/10.4141/cjas93-095
  6. Campbell GL, Bedford MR 1992 Enzyme applications for monogastric feeds: a review. Can J Anim Sci 72:449-466 https://doi.org/10.4141/cjas92-058
  7. Duncan DB 1955 Mutiple range and mutiple F test. Biometrics 11:1 https://doi.org/10.2307/3001478
  8. Feller G, Narinx E, Arpigny JL, Aittaleb M, Baise E, Genicot S, Gerday C 1996 Enzymes from psychrophilic organisms. FEMS Microbiol 18:189-202 https://doi.org/10.1111/j.1574-6976.1996.tb00236.x
  9. Gracia MI 2003 alpha-Amylase supplementation of broiler diets based on corn. Poultry Science 82(3): 436-442 https://doi.org/10.1093/ps/82.3.436
  10. Graham H, Balnave D 1995 Dietary enzyme for increasing enzyme availability. In: Biotechnology in Animal Feeds and Animal Feeding(Eds., Wallance RJ and Chesson A). Weunheim, Germany: VCH, 295-309
  11. Herbert RA 1992 The perspective on the biotechnological potential of extremophiles. Trends Biotechnol 10:395-402 https://doi.org/10.1016/0167-7799(92)90282-Z
  12. Jaroni D, Scheideler SE, Beck M, Wyatt C 1999 The effect of dietary wheat middlings and enzyme supplementation. 1. Late egg production efficiency, egg yields and egg composition in two strains of leghorn hens. Poultry Sci 78: 841-847 https://doi.org/10.1093/ps/78.6.841
  13. Lazaro R, Garcia M, Aranibar MJ, Mateos GG 2003 Effect of enzyme addition to wheat-, barley- and rye-based diets on nutrient digestibility and performance of laying hens. Br Poult Sci 44:256-265 https://doi.org/10.1080/0007166031000085616
  14. Matti Nasi 1988 Enzyme supplementation of laying hen diets based on barley and oat. Department of Animal Husbandary University of Helsinki. 00710 Helsinki. Finland
  15. NRC 1994 Nutrient requirements of poultry. National Academy Press. Washington DC
  16. Peterson RG 1985 Design and analysis of experiments. Marcel dekkor. New Yolk
  17. SAS 1996 SAS user guide. release 6.12 edition. SAS Inst Inc Cary NC. USA
  18. Sauter EA, Stadelman WJ, Harns V, Mclaren BA 1951 Methods for measuring yolk index. Poultry Sci 30:629-630 https://doi.org/10.3382/ps.0300629
  19. Yoruk MA, Gul M, Hayirli A, Karaoglu M 2006 Multi-enzyme supplementation to peak producing hens fed corn-soybean meal based diets. International Journal Poultry Sci 5:374-380 https://doi.org/10.3923/ijps.2006.374.380
  20. 김해진 민병준 조진호 진영걸 유종상 김인호 장정순 이윤교 2006 갯벌 미생물 유래 단백질 분해 효소제의 급여가 비육돈의 생산성, 아미노산 소화율, 혈액성상, 육질 특성 및 분내 휘발성 지방산과 $NH_3N$ 함량에 미치는 영향. 한국 동물자원과학회지 48:49-58 https://doi.org/10.5187/JAST.2006.48.1.049
  21. 장정순 주한승 Kumar CG 2004 고활성의 알칼리성 단백질 분해효소를 대량 생산하는 바실러스 속 I-52, 특허등록번 호 제 046582호
  22. 장정순 주한승 백승렬 김종욱 류경희 김경미 2001 갯지렁이로부터 분리한 신규한 프로테아제. 특허 공보 제9788호