Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Functions of the C-Terminal Region of Chitinase ChiCW from Bacillus cereus 28-9 in Substrate-Binding and Hydrolysis of Chitin

  • Huang, Chien-Jui (Department of Plant Pathology and Microbiology, National Taiwan University) ;
  • Chen, Chao-Ying (Department of Plant Pathology and Microbiology, National Taiwan University)
  • Published : 2006.12.30
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Abstract

In order to investigate the functions of the C-terminal region of chitinase ChiCW of Bacillus cereus 28-9, a C-terminal truncated enzyme, ChiCW$\Delta$FC, was expressed in Escherichia coli and purified to homogeneity for biochemical characterization. Compared with ChiCW, ChiCW$\Delta$FC exhibited higher chitinase activity at high temperature and pH, but expressed lower hydrolytic and binding activities toward insoluble substrates. In addition, kinetic properties indicated that ChiCW$\Delta$MC hydrolyzed oligomeric and polymeric substrates less efficiently than ChiCW. These results suggest that the C-terminal region of ChiCW plays important roles in substrate binding and hydrolysis of chitin. In addition, the biological meaning of C-terminal proteolytic modification of ChiCW is discussed.

Keywords

References

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