Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Marine Bacteria Associated with the Korean Brown Alga, Undaria pinnatifida

  • Lee, Yoo-Kyung (Polar BioCenter, Korea Polar Research Institute, KORDI) ;
  • Jung, Hyun-Jung (Polar BioCenter, Korea Polar Research Institute, KORDI) ;
  • Lee, Hong-Kum (Polar BioCenter, Korea Polar Research Institute, KORDI)
  • Published : 2006.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Several marine bacterial strains were isolated from Undaria pinnatifida (Miyok in Korean). Sixty-six strains were isolated on R2A agar media at $10^{\circ}C$ and identified by a phylogenetic analysis of the 16S rRNA gene sequences. They were grouped into 10 different sequence types based on the initial sequence analysis of the 5' domain of the gene (approximately 500 bp). Full sequences of 16S rRNA gene, were obtained from one strain in each sequence type and the species-affiliation was determined using phylogenetic and sequence similarity analyses. The results of the analyses indicated that they were closely related to Psychrobacter aquimaris, P. celer, P. nivimaris, P. pulmonis, Psychromonas arctica or Bacillus psychrodurans. These bacteria are marine or psychrotrophic bacteria. Because the sporophytes of U. pinnatifida are cultured on the costal area during winter, the U. pinnatifida-associated bacteria appeared to grow at low temperatures. U. pinnatifida sporophytes can be a good source for the isolation of psychrotrophic bacteria.

Keywords

References

  1. Abd El-Rahman, H.A., D. Fritze, C. Sproer, and D. Claus. 2002.,Two novel psychrotolerant species, Bacillus psychrotolerans sp. nov. and Bacillus psychrodurans sp. nov., which contain ornithine in their cell walls. Int. J. Syst. Evol. Microbiol. 52, 2127-2133 https://doi.org/10.1099/ijs.0.01665-0
  2. Bennamara, A., A. Abourriche, M. Berrada, M. Charrouf, N. Chaib, M. Boudouma, and F.X. Garneau. 1999. Methoxybifurcarenone: an antifungal and antibacterial meroditerpenoid from the brown alga Cystoseira tamariscifolia. Phytochemistry 52, 37-40 https://doi.org/10.1016/S0031-9422(99)00040-0
  3. Brown, B.J. and J.F. Preston, 3rd. 1991. L-guluronan-specific alginate lyase from a marine bacterium associated with Sargassum. Carbohydr. Res. 211, 91-102 https://doi.org/10.1016/0008-6215(91)84148-8
  4. Chun, J. 1995. Computer-assisted classification and identification of actinomycetes. Ph.D. Thesis, University of Newcastle, Newcastle upon Tyne, UK
  5. Felsenstein, J. 1993. PHYLIP (Phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, WA, USA
  6. Groudieva, T., R. Grote, and G. Antranikian. 2003. Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen. Int. J. Syst. Evol. Microbiol. 53, 539-545 https://doi.org/10.1099/ijs.0.02182-0
  7. Guiry, M.D. and G. Blunden (eds). 1991. Seaweed resources in Europe: uses and potential. Chichester, UK: John Wiley and Sons
  8. Heuchert, A., F.O. Glockner, R. Amann, and U. Fischer. 2004. Psychrobacter nivimaris sp. nov., a heterotrophic bacterium attached to organic particles isolated from the South Atlantic (Antarctica). Syst. Appl. Microbiol. 27, 399-406 https://doi.org/10.1078/0723202041438455
  9. Ivanova, E.P., R. Christen, Y.V. Alexeeva, N.V. Zhukova, N.M. Gorshkova, A.M. Lysenko, V.V. Mikhailov, and D.V. Nicolau. 2004a. Brevibacterium celere sp. nov., isolated from degraded thallus of a brown alga. Int. J. Syst. Evol. Microbiol. 54, 2107-2111 https://doi.org/10.1099/ijs.0.02867-0
  10. Ivanova, E.P., Y.A. Alexeeva, N.V. Zhukova, N.M. Gorshkova, V. Buljan, D.V. Nicolau, V.V. Mikhailov, and R. Christen. 2004b. Bacillus algicola sp. nov., a novel filamentous organism isolated from brown alga Fucus evanescens. Syst. Appl. Microbiol. 27, 301-307 https://doi.org/10.1078/0723-2020-00269
  11. Ivanova, E.P., Y.V. Alexeeva, S. Flavier, J.P. Wright, N.V. Zhukova, N.M. Gorshkova, V.V. Mikhailov, D.V. Nicolau, and R. Christen. 2004c. Formosa algae gen. nov., sp. nov., a novel member of the family Flavobacteriaceae. Int. J. Syst. Evol. Microbiol. 54, 705-711 https://doi.org/10.1099/ijs.0.02763-0
  12. Johansen, J.E., P. Nielsen, and C. Sjoholm. 1999. Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of Cytophaga lytica to Cellulophaga lytica gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 49, 1231-1240 https://doi.org/10.1099/00207713-49-3-1231
  13. Lee, Y.K., D.J. Lim, Y.H. Lee, and Y.-I. Park. 2006. Variation in fucoidan contents and monosaccharide compositions of Korean Undaria pinnatifida. Algae 21, 157-160 https://doi.org/10.4490/ALGAE.2006.21.1.157
  14. Nagayama, K., Y. Iwamura, T. Shibata, I. Hirayama, and T. Nakamura. 2002. Bactericidal activity of phlorotannins from the brown alga Ecklonia kurome. J. Antimicrob. Chemother. 50, 889-893 https://doi.org/10.1093/jac/dkf222
  15. Nedashkovskaia, O.I., E.P. Ivanova, IIu Bakunina, V.I. Svetashev, T.N. Zviagintseva, and V.V. Mikhailov. 2002. Characterization of marine bacteria Pseudoalteromonas citrea degradating fucoidan. Microbiol. 64, 3-10
  16. Okamura, K. 1936. Nippon Kaiso Shi (Descriptions of Japanese algae). Tokyo. pp. 964
  17. Preston, J.F. 3rd, T. Romeo, J.C. Bromley, R.W. Robinson, and H.C. Aldrich. 1985. Alginate lyase-secreting bacteria associated with the algal genus Sargassum. Dev. Ind. Microbiol. 26, 727-740
  18. Preston, J.F. 3rd, T. Romeo, and J. C. Bromley. 1986. Selective alginate degradation by marine bacteria associated with the algal genus Sargassum. J. Ind. Microbiol. 1, 235-244 https://doi.org/10.1007/BF01569277
  19. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425
  20. Sawabe, T., R. Tanaka, M.M. Iqbal, K. Tajima, Y. Ezura, E.P. Ivanova, and R. Christen. 2000. Assignment of Alteromonas elyakovii KMM 162T and five strains isolated from spot-, wounded fronds of Laminaria japonica to Pseudoalteromonas elyakovii comb. nov. and the extended description of the species. Int. J. Syst. Evol. Microbiol. 50, 265-271 https://doi.org/10.1099/00207713-50-1-265
  21. Thompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680 https://doi.org/10.1093/nar/22.22.4673
  22. Vela, A.I., M.D. Collins, M.V. Latre, A. Mateos, M.A. Moreno, R. Hutson, L. Dominguez, and J.F. Fernandez-, Garayzabal. 2003. Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs. Int. J. Syst. Evol. Microbiol. 53, 415-419 https://doi.org/10.1099/ijs.0.02413-0
  23. Yoon, J.H., C.H. Lee, S.J. Kang, and T.K. Oh. 2005a. Psychrobacter celer sp. nov., isolated from sea water of the South Sea in Korea. Int. J. Syst. Evol. Microbiol. 55, 1885-1890 https://doi.org/10.1099/ijs.0.63682-0
  24. Yoon, J.H., C.H. Lee, S.H. Yeo, and T.K. Oh. 2005b. Psychrobacter aquimaris sp. nov. and Psychrobacter namhaensis sp. nov., isolated from sea water of the South Sea in Korea. Int. J. Syst. Evol. Microbiol. 55, 1007-1013 https://doi.org/10.1099/ijs.0.63464-0
  25. Zhang, D.M., G.R. Miao, and L.Q. Pei. 1984. Studies on Undaria pinnatifida. Hydrobiologia 116/117, 263-265 https://doi.org/10.1007/BF00027681