Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Carbon Source-Dependent Regulation of the Schizosaccharomyces pombe pbh1 Gene

  • Kim, Su-Jung (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Cho, Nam-Chul (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Ryu, In-Wang (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Kim, Kyung-Hoon (Division of Life Sciences, College of Natural Sciences, Kangwon National University) ;
  • Park, Eun-Hee (College of Pharmacy, Sookmyung Women's University) ;
  • Lim, Chang-Jin (Division of Life Sciences, College of Natural Sciences, Kangwon National University)
  • Published : 2006.12.31
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Abstract

Pbh1, from the fission yeast Schizosaccharomyces pombe, is a baculoviral inhibitor of apoptosis (IAP) repeat (BIR) domain-containing protein. Its unique encoding gene was previously found to be regulated by nitric oxide and nitrogen starvation. In the current work, the Pbh1-lacZ fusion gene was used to elucidate the transcriptional regulation of the pbh1 gene under various carbon sources. When fermentable carbon sources, such as glucose (at a low concentration of 0.2 %), sucrose (2.0 %) and lactose (2.0 %), were the sole carbon source, the synthesis of $\beta$-galactosidase from the Pbh1-lacZ fusion gene was reasonably enhanced. However, the induction by these fermentable carbon sources was abolished in the Pap1-negative S. pombe cells, implying that this type of induction of the pbh1 gene is mediated by Pap1. Ethanol (2.0%), a nonfermentable carbon source, was also able to enhance the synthesis of $\beta$-galactosidase from the fusion gene in wild-type cells but not in Pap1-negative cells. The results indicate that the S. pombe pbh1 gene is up-regulated under metabolic oxidative stress in a Pap1-dependent manner.

Keywords

References

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