Journal of Microbiology

  • 제44권5호
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  • Pages.523-529
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  • 2006
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Characterization of Osh3, an Oxysterol-binding Protein, in Filamentous Growth of Saccharomyces cerevisiae and Candida albicans

  • Hur, Hyang-Sook (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Ryu, Ji-Ho (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Kwang-Hoon (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Jin-Mi (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University)
  • 발행 : 2006.10.31
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초록

OSH3 is one of the seven yeast homologues of the oxysterol binding proteins (OSBPs) which have the major binding affinity to the oxysterols and function as regulator of cholesterol biosynthesis in mammals. Mutational analysis of OSH3 showed that OSH3 plays a regulatory role in the yeast-to-hyphal transition through its oxysterol-binding domain in Saccharomyces cerevisiae. The OSH3 gene was also identified in the pathogenic yeast Candida albicans. Deletion of OSH3 caused a defect in the filamentous growth, which is the major cause of the C. albicans pathogencity. The filamentation defect of the mutation in the MAPK-associated transcription factor, namely $cph1{\Delta}$ was suppressed by overexpression of OSH3. These findings suggest the regulatory roles of OSH3 in the yeast filamentous growth and the functional conservations of OSH3 in S. cerevisiae and C. albicans.

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참고문헌

  1. Adams, A., D.E. Gottschling, C.A. Kaiser, and T. Stearn. 1997. Methods in yeast genetics. Cold Spring Harbor Press, Cold Spring Harbor, NY, USA
  2. Beh, C.T., L. Cool, J. Phillips, and J. Rine. 2001. Overlapping functions of the yeast oxysterol-binding protein homologues. Genetics 157, 1117-1140
  3. Christianson, T.W., R.S. Sikorski, M. Dante, J.H. Shero, and P. Hieter. 1992. Multifunctional yeast high-copy-number shuttle vectors. Gene 110, 119-122 https://doi.org/10.1016/0378-1119(92)90454-W
  4. Fang, M., B.G. Keams, A. Gedvilaite, S. Kagiwada, M. Kearns, M.K. Fung, and V.A. Bankaitis. 1996. Kes 1 p shares homology with human oxysterol binding protein and participates in a novel regulatory pathway for yeast Golgi-derived transport vesicle biogenesis. EMBO J. 15, 6447-6459
  5. Feng, Q., E. Summers, B. Guo, and G.R. Fink. 1999. Ras signalling is required for serum-induced hyphal differentiation in Candida albicans. J. Bacteriol. 181, 6339-6346
  6. Fonzi, W. and M. Irwin. 1993. Isogenic strain construction and gene mapping in Candida albicans. Genetics 134, 717-728
  7. Gimeno, C.J., P.O. Ljungdahl, C.A. Styles, and G.R. Fink. 1992. Unipolar cell divisions in the yeast S. cerevisiae lead to filamentous growth: regulation by starvation and RAS. Cell 68, 1077-1090 https://doi.org/10.1016/0092-8674(92)90079-R
  8. Goldstein, J.L. and M.S. Brown. 1990. Regulation of the mevalonate pathway. Nature 343, 425-430 https://doi.org/10.1038/343425a0
  9. Hull, C.M. and A.D. Johnson. 1999. Identification of a mating type-like locus in the asexual pathogenic yeast Candida albicans. Science 285, 1271-1275 https://doi.org/10.1126/science.285.5431.1271
  10. Jiang, B., J.L. Brown, J. Sheraton, N. Fortin, and H. Bussey. 1994. A new family of yeast genes implicated in ergosterol synthesis is related to the human oxysterol binding protein. Yeast 10, 341-353 https://doi.org/10.1002/yea.320100307
  11. Levanon, D., C.L. Hsieh, U. Francke, P.A. Dawson, N.D. Ridgway, M.S. Brown, and J.L. Goldstein. 1990. cDNA cloning of human oxysterol-binding protein and localization of the gene to human chromosome 11 and mouse chromosome 19. Genomics 7, 65-74 https://doi.org/10.1016/0888-7543(90)90519-Z
  12. Liu, H., J. Kohler, and G.R. Fink. 1994. Suppression of hyphal formation in Candida albicans by mutation of a STE12 homolog. Science 266, 1723-1726 https://doi.org/10.1126/science.7992058
  13. Liu, H., C.A. Styles, and G.R. Fink. 1993. Elements of the yeast pheromone response pathway required for filamentous growth of diploids. Science 262, 1741-1744 https://doi.org/10.1126/science.8259520
  14. Pan, X. and J. Heitman. 1999. Cyclic AMP-dependent protein kinase regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Mol. Cell BioI. 19, 4874-4887 https://doi.org/10.1128/MCB.19.7.4874
  15. Park, Y.U., O. Hwang, and J. Kim. 2002. Two-hybrid cloning and characterization of OSH3, a yeast oxysterol-binding protein homolog. Biochem. Biophys. Res. Commun. 293, 733-740 https://doi.org/10.1016/S0006-291X(02)00288-7
  16. Ridgway, N.D., P.A. Dawson, Y.K. Ho, M.S. Brown, and J.L. Goldstein. 1992. Translocation of oxysterol binding protein to Golgi apparatus triggered by ligand binding. J. Cell Biol. 116, 307-319 https://doi.org/10.1083/jcb.116.2.307
  17. Roberts, R.L. and G.R. Fink. 1994. Elements of a single MAP kinase cascade in Saccharomyces cerevisiae mediate two developmental programs in the same cell type: mating and invasive growth. Genes Dev. 8, 2974-2985 https://doi.org/10.1101/gad.8.24.2974
  18. Scherer, S. and P. Magee. 1990. Genetics of Candida albicans. Microbiol. Rev. 54, 226-241
  19. Shepherd, M., R. Poulter, and P. Sullivan. 1985. Candida albicans: biology, genetics, and pathogenecity. Ann. Rev. Microbiol. 39, 579-614 https://doi.org/10.1146/annurev.mi.39.100185.003051
  20. Sikorski, R.S. and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27
  21. Stoldt, V.R., A. Sonneborn, C.E. Leuker, and J.P. Ernst. 1997. Efg1p, an essential regulator of morphogenesis of the human pathogen Candida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi. EMBO J. 16, 1982-1991 https://doi.org/10.1093/emboj/16.8.1982
  22. Xu, Y., Y. Liu, N.D. Ridgway, and C.R. McMaster. 2001. Novel members of the human oxysterol-binding protein family bind phospholipids and regulate vesicle transport. J. BioI. Chem. 276, 18407-18414 https://doi.org/10.1074/jbc.M101204200
  23. Yano, T., M. Inukai, and P. Isono. 2004. Deletion of OSH3 gene confers resistance against ISP-1 in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 315, 228-234 https://doi.org/10.1016/j.bbrc.2004.01.039