Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effects of Pear Extracts Cultured Under Conventional and Environment-friendly Conditions on Cell Proliferation in Rat Hepatocytes

친환경 배 및 관행재배 배 추출물이 간세포 성장에 미치는 효과

  • Yoon, Byung-Chul (Environmental-Friendly Agriculture Research Center,Bio-therapy Human Resources Center,Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Kil-Yong (Environmental-Friendly Agriculture Research Center,Division of Biological and Environmental Chemistry, School of Agriculture and Life Science, Chonnam National University) ;
  • Park, Soo-Hyun (Environmental-Friendly Agriculture Research Center,Bio-therapy Human Resources Center,Department of Veterinary Physiology, College of Veterinary Medicine, Chonnam National University)
  • 윤병철 (친환경 농업연구사업단,전남대학교 수의과대학 생리학교실,바이오 치료 산업인력 양성팀) ;
  • 김길용 (친환경 농업 연구사업단,전남대학교 농업 생명과학대학) ;
  • 박수현 (친환경 농업연구사업단,전남대학교 수의과대학 생리학교실,바이오 치료 산업인력 양성팀)
  • Published : 2006.09.30
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Abstract

In the present study, we compared the functional analysis of pear extracts cultured in conventional and environment-friendly conditions in primary cultured rat hepatocytes. ATP synthesis significantly increased by the treatment with environment friendly cultured pear powder but not by conventional group. In addition, cell proliferation using $[^3H]$-thymidine incorporation was also stimulated by environment-friendly cultured pear extract compared to conventional group. Moreover, the expressions of CDK-2 and CDK-4 were increased but p21WAF1/Cip1 and p27 Kip1 decreased by environment-friendly cultured pear extract but not by conventional group. In conclusion, environment-friendly cultured pear powder has stimulatory effect on cell proliferation compared to conventional group in primary cultured rat hepatocytes.

Sprague-Dawley 랫트(실험용 쥐)에서 간을 적출하여 Collagen coating된 세포 배양 접시에 간세포 배양을 하여 친환경 재배 배 추출물과 일반 관행 재배 배 추출물의 간세포 기능성을 분석하였다. 3일간 적응 시킨 후 관행 재배 배 추출물과 친환경 재배 배 추출물을 농도 별(동결 건조된 $5\;{\mu}g/ml$, $20\;{\mu}g/ml$, $40\;{\mu}g/ml$) 처리하여 간세포의 에너지원인 ATP assay를 실시한 결과 관행 재배 배 추출물의 경우는 대조군과 유의한 차이는 인정이 되지 않았으나 친환경 재배 배 추출물의 경우 농도 의존적으로 정상군에 비해 180%까지 증가하는 것으로 나타났다. 한편 세포 성장의 경우도 DNA의 특유 염기 구성성분중의 하나인 thymine의 전구체인 $[^3H]$-thymidine을 간세포에 처리하여 DNA 합성을 알아본 결과 친환경 재배 배 추출물 을 농도별로 처리하였을 때 관행재배 배 추출물에 비해 간세포의 세포 성장율이 증가하는 것으로 나타났다($40\;{\mu}g/ml$ 투여 시 관행 재배 배추출물 112% vs. 친환경 재배 배 추출물은 234%; p<0.05). 아울러 세포성장 단백질인 CDK-2, CDK-4의 경우도 친환경 배 추출물 처리 시 현저하게 증가하는 것으로 나타났으며 세포성장 억제 단백질인 p21WAF1/Cip1 및 p27 Kip1의 경우는 억제시키는 것으로 나타났다.

Keywords

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