Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지

Accuracy and Precision of Spectrophotometric Measurement of Clay Content in Soils

분광광도계를 이용한 점토함량 분석의 정확성 및 정밀성 평가

  • Park, Soon-Nam (Department of Environmental Horticulture, The University of Seoul) ;
  • Kim, Kye-Hoon (Department of Environmental Horticulture, The University of Seoul) ;
  • Kang, Ji-Young (National Instrumentation Center for Environmental Management, Seoul National University)
  • 박순남 (서울시립대학교 자연과학대학 환경원예학과) ;
  • 김계훈 (서울시립대학교 자연과학대학 환경원예학과) ;
  • 강지영 (서울대학교 농업생명과학대학 농업과학공동기기센터)
  • Published : 2006.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Accuracy and precision of the spectrophotometeric analysis of clay content in soils were estimated by comparison with the conventional pipet method. Clay contents in 25 soil samples of various physico-chemical properties including texture were determined by spectrophotometry and pipet methods, and the two sets of data an clay content were compared by several statistical analyses. The correlation between clay contents determined by spectrophotometry and pipet methods was highly significant. The regression coefficient was $0.98^{**}$ and the slope of regression equation was close to 1.0. The standard deviation and CV of clay contents measured by spectorphotometry were smaller than those found in the data of clay contents obtained by pipet method. In conclusion, compared to the conventional pipet method, spectrophotometry was a rapid, convenient, accurate and precise method for the measurement of clay content in soils.

본 연구는 분광광도계를 이용하여 토양중 점토함량을 분석하는 방법의 정확성과 정밀성을 평가하기 위하여 수행하였다. 이를 위하여 분광광도계를 이용하는 방법과 피펫법을 이용하여 동일한 시료의 점토함량을 측정하고 두 방법간의 정확성과 정밀성을 비교하였다. 두 방법으로 동일 시료의 점토함량을 분석한 결과 상관관계는 $y=x-0.0002(r=0.98^{**})$로 매우 높았고 기울기는 1에 가까웠으며 절편은 0에 가까워 정확성에는 거의 차이가 없는 것으로 나타났다. 또한, 점토함량에 대한 두 방법간 정밀성을 비교하기 위하여 표준편차와 변이계수를 조사한 결과 분광광도법의 표준편차와 변이계수가 피펫법보다 더 낮게 조사되었다. 따라서 분광광도계를 이용하면 시간과 공간을 절약하고 신속하고 정확하게 토양 중 점토함량을 분석할 수 있을 것으로 판단된다.

Keywords

References

  1. Bouyoucos, G. J. (1933) A comparison between the pipette method and the hydrometer method for making mechanical analysis of soils, with new directions. Soil Sci. 38, 335-343
  2. Daddah, M. T. (1974) The hydrometer method for detailed particle-size anslysis: 1. Graphical interpretation of hydrometer readings and test of method. Soil Sci. 118, 102-108 https://doi.org/10.1097/00010694-197408000-00007
  3. Day, P. R. 1950. Physical basis of particle size analysis by the hydrometer method. Soil Sci. 70, 363-374 https://doi.org/10.1097/00010694-195011000-00004
  4. Gee, G. W. and Bauder, J. W. (1986) Particle size analysis. In Methods of Soil Analysis Part 1, A. Klute (2nd ed.) pp. 383-411. American Society of Agronomy, Madison, WI
  5. Hyun, B. K., Kim, M. S., Eom, K. C. and Jo, I. S. (2000) A more simplified hydrometer method for soil texture analysis. J. Kor. Soc. Soil Sci. Fert. 33, 153-159
  6. Jo, I. S., Kim, L. Y., Ryu, K. S., Im, J. N., Um, K. T. and Kwag, Y. S. (1984) Design and performance of the modified hydrometer for the rapid measurement of clay content in soils. J. Kor. Soc. Soil Sci. Fert. 17, 325-329
  7. Kilmer, V. J. and Alexander, L. T. (1949) Methods of making mechanical analyses of soils. Soil Sci. 68, 15-24 https://doi.org/10.1097/00010694-194907000-00003
  8. Liu, T. K., Odell, R. T., Etter, W. C. and Thornburn, T. H. (1966) A comparison of clay contents determined by hydrometer and pipette methods using reduced major axis analysis. Soil Sci. Soc. Am. Proc. 30, 665-669 https://doi.org/10.2136/sssaj1966.03615995003000060008x
  9. Miller, W. P. and Miller, D. M. (1987) A micro-pipette method for soil mechanical analysis. Commun. Soil Sci. Plant Anal. 18, 1-15 https://doi.org/10.1080/00103628709367799
  10. Sur, H. S. and Kukal, S. S. (1992) A modified hydrometer procedure for particle size analysis. Soil Sci. 153, 1-4 https://doi.org/10.1097/00010694-199201000-00001
  11. Allen, T. (1997) In Particle size measurement. (5th ed.) Chapman and Hall, New York
  12. Beuselinck, L., Govers, G., Poesen, J. and Degraer, G. (1998) Grain-size analysis by laser diffractometry: comparison with the sieve-pipette method. Catena 32, 193-208 https://doi.org/10.1016/S0341-8162(98)00051-4
  13. Buurman, P., Pape, T. and Muggler, C. C. (1997) Laser grainsize determination in soil genetic studies 1. Practical problems. Soil Sci. 162, 211-218 https://doi.org/10.1097/00010694-199703000-00007
  14. Coakley, J. P. and Syvitski, J. P. M. (1991) Sedigraph technique. In Principles, methods, and application of particle size analysis. J. P. M. Syvitski. pp. 130-142. Cambridge University Press, New York
  15. Muggler, C. C., Pape, T. and Buurman, P. (1997) Laser-grain size determination in soil genetic studies 2. Clay content, clay formation, and aggregation in some Brazilian oxisols. Soil Sci. 162, 219-228 https://doi.org/10.1097/00010694-199703000-00008
  16. Naime, J. M., Vaz, C. M. P. and Macedo, A. (2001) Automated soil particle size analyzer based on gamma-ray attenuation. Comput. Electron. Agric. 31, 295-304 https://doi.org/10.1016/S0168-1699(00)00188-5
  17. Park S. N., Kim, K. H. and Kang, J. Y. (2005) Use of spectrophotimetry for quantitative determination of soil clay content. J. Korean. Appl. Biol. Chem. 48, 183-188
  18. Pennington, K. L. and Lewis, G. C. (1979) A comparison of electronic and pipet methods for mechanical analysis of soils. Soil Sci. 128, 280-284 https://doi.org/10.1097/00010694-197911000-00004
  19. Welch, N. H., Allen, P. B. and Galindo, D. J. (1979) Particlesize analysis by pipette and SediGraph. J. Environ. Qual. 8, 543-546 https://doi.org/10.2134/jeq1979.00472425000800040020x
  20. Chung, J. B., Kim, M. K., Kim, B. J. and Kim, K. H. (1999) Comparison of micro-pipette method and hydrometer method in soil particle size analysis. J. Kor. Soc. Soil Sci. Fert. 32, 274-278
  21. Stockham, J. D. and Fochtman, E. G. (1979) In Particle size analysis. (3rd ed.) Ann Arbor Science, Michigan
  22. Vaz, C. M. P., Oliveira, J. C. M., Reichardt, K., Crestana, S., Cruvinel, P. E. and Bacchi, O. O. S. (1992) Soil mechanical analysis through gamma ray attenuation. Soil Technol. 5, 319-325
  23. Elias, E. A., Bacchi, O. O. S. and Reichardt, K. (1999) Alternative soil particle-size analysis by gamma-ray attenuation. Soil Tillage Res. 52, 121-123 https://doi.org/10.1016/S0167-1987(99)00070-7
  24. Oliverira, J. C. M., Vaz, C. M. P., Reichardt, K. and Swartzendruber, D. (1997) Improved soil particle-size analysis by gamma-ray attenuation. Soil Sci. Soc. Am. J. 61, 23-26 https://doi.org/10.2136/sssaj1997.03615995006100010004x