Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Genetic Analysis of Polygonati Rhizoma and Polygonati odorati Rhizoma using Random Amplified Microsatellite Polymorphism

RAMP를 이용한 황정과 위유의 유전적 분석

  • Published : 2006.06.30
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Abstract

Two herbal medicines of the Polygonatum genus, namely Polygonati Rhizoma and Polygonati Odorati Rhizoma, are difficult to distinguish from each other through exterior morphologic aspects. Furthermore, because the standard components for physiochemical distinction have not yet been standardized, the identification of these medicines through botanic taxology is based on genetic methods of random amplified microsatellite polymorphism (RAMP). For the RAMP evaluation, we used five sets of UBC microsatellite primers 811, 818, 834, 836, 842 and random primer M1. Although no specific band that could clearly distinguish Polygonati Rhizoma from Polygonati Odorati Rhizoma was found, 11 Polygonatum plants could be divided into two groups by this method. P. sibiricum and P. stenophyllum were classified to group I and the others were to group II. As P. sibiricum and P. stenophyllum were very similar in genetic and morphologic perspective, the results suggest that P. stenophyllum belongs to the Polygonati Rhizoma family.

형태학적으로 구분이 어려운 황정과 위유의 범위를 구분하기 위해 황정에 속하는 층층갈고리둥굴레, 다화황정, 진황정 등 3종과 위유에 속하는 8종의 둥굴레 동속 식물 등 11종 23 개체 시료로 RAMP분석한 결과를 요약하면 다음과 같다. 1. 황정그룹과 위유그룹은 NTSYS를 통한 유연관계 분석에서 55%의 유연관계를 나타냈으며, 층층갈고리둥굴레와 층층둥굴레는 81.75%로 분석에 사용된 둥글레 동속 식물 중 가장 높은 유연관계를 나타냈다. 2. 둥굴레 동속식물에 해당하는 층층둥글레는 황정의 약재인 층층갈고리둥굴레와 외부형태학적으로, 그리고 다형성패턴결과가 매우 유사하므로 황정의 약재에 속하는 것으로 사료된다. 3. 진황정은 위유와 외부형태적으로 유사하며, 유연관계분석에서도 위유의 그룹에 속하므로 위유의 약재에 대한 구분을 새롭게 할 필요성이 있다.

Keywords

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