Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Simple Sequence Repeat Markers Linked to Quantitative Trait Loci Controlling Seed Weight, Protein and Oil Contents in Soybean

콩에서 종실의 무게와 oil 및 단백질 함량을 조절하는 양적 형질 유전자좌와 연관된 simple sequence repeat marker

  • 김현경 (농촌진흥청 작물과학원 영남농업연구소) ;
  • 강성택 (농촌진흥청 작물과학원 작물기능개발과) ;
  • 정명근 (강원대학교 한방보건복지대학 생약자원개발학과) ;
  • 정찬식 (농촌진흥청 작물과학원 영남농업연구소) ;
  • 오기원 (농촌진흥청 작물과학원 영남농업연구소) ;
  • 백인열 (농촌진흥청 작물과학원 영남농업연구소) ;
  • 손병구 (부산대학교 생명자원과학부)
  • Published : 2006.10.01
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Abstract

Soybean [Glycine max (L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvement of the quality and quantity of soybean seed constituents is one of the most important objectives in soybean breeding. Protein content and seed size are important properties to determine the quality of tofu and soy sprouts respectively. The objective of this study was to identify quantitative trait loci (QTLs) that control seed weight, protein and oil content in soybean. The 117 $F_{2:10}$ recombinant inbred lines (RlL) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Narrow-sense heritability estimates based on a plot mean on seed weight, protein and oil content were 0.8, 0.78 and 0.71, respectively. Four independent QTLs for seed weight were identified from linkage group (LG) F, I and K. Five QTL for protein content were located on LG D1b, E, H, I and L. Oil content was related with six QTLs located on LG D1b, E, G, I, J and N. Protein and oil content have three common QTLs on LG D1b, E and I. Thus, we identified major loci improving soybean seed quality.

콩은 전 세계 시장의 48%를 차지하는 중요한 유료작물이다. 콩 종자를 구성하는 양적인 부분과 질적인 부분의 개선은 콩 육종 목표의 중요한 부분이다. 단백질함량과 종실의 크기는 두부와 콩나물의 질을 평가하는 중요한 특성이다. 따라서 본 연구는 콩에서 종실의 크기와 단백질 및 oil 함량을 조절하는 양적형질유전자좌(QTLs)를 확인하기 위하여 실시하였다. 시험재료로는 큰올콩과 신팔달콩을 교배한 후 $F_2$유래 $F_10$세대의 RIL을 이용하였으며, 이를 바탕으로 종실의 무게와 oil 및 단백질 함량과 관련된 QTLs를 탐색하였다. 종실의 무게와 단백질 및 oil 함량의 협의의 유전력은 각각 0.8과 0.78 및 0.71을 나타내었다. 종실의 무게와 관련된 QTLs는 연관군 F, I와 K의 세 개의 독립적인 QTLs를 확인하였다. 단백질함량과 관련된 QTLs는 연관군 D1b, E, H, I와 L의 다섯 개의 독립적인 QTLs를 확인하였다. 그리고 oil 함량과 관련된 QTLs는 연관군 D1b, E, G, I, J와 N의 여섯 개의 독립적인 QTLs를 확인하였다. oil 및 단백질 함량과 관련된 QTLs는 연관군 D1b, E와 I에서 공통적으로 나타났다. 따라서 이들 주요 QTL은 콩 품종 육성과정에서 품질의 개선하기 위한 선발 마커로서 활용가치가 높은 것으로 판단된다.

Keywords

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