생명과학회지 (Journal of Life Science)

  • 제16권4호
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  • Pages.640-646
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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장염비브리오 항균활성을 위한 탱자와 매실의 추출조건 최적화

Extraction Process Optimization of Poncirus trifoliata and Prunus mume for Antibacterial Activity against Vibrio parahaemolyticus

  • 발행 : 2006.07.31
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초록

비브리오식중독의 피해를 저감시키는 기능성 식품소재를 개발하기 위하여, 예비실험에서 V. parahaemolyticus균에 항균활성이 우수한 것으로 밝혀진 탱자와 매실의 최적 추출조건을 조사하였다. 먼저, 열수로 추출한 실험에서 탱자의 최적 조건은 $80{\sim}100^{\circ}C$에서 30분간, 매실의 경우 $100^{\circ}C$에서 30분간 가열 추출한 시험액의 저지환이 각각 14.8 및 16.6 mm로써, 최대의 항균활성을 나타내었다. 또한 NaOH, $K_2CO_3$ 및 $NaHCO_3$의 알칼리 식품첨가물을 첨가하여 추출한 실험에서, NaOH를 첨가한 경우 탱자의 항균활성은 증진되지 않았으나 매실의 항균활성은 다소 증진되었으며, NaOH 이외 알칼리들은 공시 식물체들의 항균활성을 감소시켰다. NaOH의 첨가농도를 단계적으로 달리하고 80, 100 및 $120^{\circ}C$에서 추출한 결과, 탱자의 경우 앞서의 열수추출 결과와 별다른 차이가 없었으나, 매실은 NaOH를 0.05N 농도로 첨가하고 $120^{\circ}C$ 에서 추출한 조건에서 저지환 직경 19.9 mm의 최대 항균활성을 나타내었다. 탱자와 매실을 주정으로 진탕추출하고 주정을 제거하여 주정추출물을 얻었다. 이 추출물에 NaOH를 단계적으로 첨가하고 $100^{\circ}C$에서 30분간 물로 재추출한 결과, 주정추출물의 항균활성은 전반적으로 물추출한 결과보다 낮고 NaOH의 첨가량이 많을수록 활성의 감소를 나내었다. 따라서 본 실험에서, 탱자의 최적추출조건은 $80{\sim}100^{\circ}C$의 열수로 30분간, 매실은 NaOH를 0.05N 농도로 첨가한 $120^{\circ}C$의 열수로 30분간 추출하는 것이 가장 효율적이라는 결론을 얻었다.

The present investigation was carried out particularly to optimize the extraction process of Poncirus trifoliata and Prunus mume to develop a functional foodstuff having antibacterial activity against Vibrio parahaemolyticus. The extracts were prepared from the fruits under various conditions, and then optimum extraction conditions were decided in case maximal antibacterial activity was obtained. In extraction process by using hot water, the best antibacterial activity of Poncirus trifoliata was achieved at $80{\sim}100^{\circ}C$ for 30 min, while that of Prunus mume was achieved at $100^{\circ}C$ for 30 min, as showing 14.8 and 16.6 mm of inhibition diameter, respectively. The extraction process with addition of NaOH increased the activity of Prunus mume, but addition of $K_2CO_3$ and $NaHCO_3$ drastically decreased the activity. In the processes at high temperature up to $120^{\circ}C$ by using pressure extractor and with various concentrations of NaOH, the maximum activity was observed in the extract of Prunus mume at $120^{\circ}C$ for 30 min with 0.05 N NaOH. The fermented ethanol extract of the fruits showed less activity than those of the extracts with water and NaOH solution.

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