Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Antimicrobial effect of Kimchi ingredients of methanol extract on pathogenic microorganisms

김치 재료 methanol 추출물이 식품유해 미생물에 미치는 항균효과

  • Shin Sun-Mi (Department of Food & Nutrition, Sungshin Woman's University) ;
  • Park Ju-Yeon (Department of Food & Nutrition, Sungshin Woman's University) ;
  • Hahn Young-Sook (Department of Food & Nutrition, Sungshin Woman's University)
  • 신선미 (성신여자대학교 식품영양학과) ;
  • 박주연 (성신여자대학교 식품영양학과) ;
  • 한영숙 (성신여자대학교 식품영양학과)
  • Published : 2005.02.01
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Abstract

This study was carried out to determine the inhibitory effect of methanol extract from kimchi ingredients against Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, and Esherichia coli which are pathogenic microorganisms and Aspergillus sp. Penicillium sp. Antimicrobial activity of methanol extracts was tested against bacteria by paper disc method. Antifungal activity of methanol extracts was shown by hyphal growth inhibition ratio. The methanol extracts from all materials were effective against E. coli among them. And the antimicrobial activity of the methanol extracts from ginger and onion were lower than the others. The antifungal activity of the methanol extracts from radish, ginger, and garlic were effective against Aspergillus sp. and Penicillium sp. In the result of identifying antimicrobial effect rate, the methanol extracts from red pepper and radish had more than $40\%$ against S. typhimurium and more than $30\%$ against E. coli. Also the methanol extracts from onion had an high inhibitory effect rate of more than $50\%$ against S. typhimurium and that from garlic had more than $60\%$ against S. aureus. The minimum inhibitory concentration(MIC) of red pepper was examined 500 $\mu$g/mL against L. monocytogenes. This value was the lowest among the others.

4종류의 식품 위해 세균 Salmonella typhimurium, Listeria monocytogenes, Staphylococcus aureus, Esherichia coli과 김치 부패에 관여하는 곰팡이 Aspergillus sp. Penicillium sp.에 대하여 김치 재료에 의한 항균성을 조사하였다. Paper disc method로 측정한 생육저해환 결과 4 종류의 세균에 대하여 각 재료의 methanol 추출물들은 E. coli에 대해서 재료에 따라 약간의 차이는 있으나 약 20.00 mm에 가까운 항균성을 보이는 경향을 알 수 있었으며 생강과 양파는 다른 재료와 비교해볼 때 형성된 clear zone의 크기가 작은 경향을 확인할 수 있었다. 곰팡이에 대한 결과 Aspergillus sp.과 Penicillium sp.에 대하여 마늘 추출물이 가장 우수하였고, 생강 추출물과 무 추출물도 우수한 항균 작용을 나타내었다. S. typhimurium에 대하여 농도별 생육저해는 무, 양파 추출물이 균의 중식이 활발히 일어나는 대수중식기인 4$\~$12시간 사이에 생육을 억제하는 것으로 나타났고 고춧가루 추출물도 1,000 ppm의 농도에서 S. typhimurium의 생육을 크게 억제하는 것으로 나타났으며 L. monocytogenes의 생육에 미치는 영향은 S. typhimurium에 대한 결과 유사하였으며 paper disc로 조사한 생육 저해환의 결과와 일치하는 경향을 보였다. 반면 전체적으로 김치 재료 methanol 추출물은 S. aureus 생육 저해에 큰 영향을 미치지 못했으나 특별히 1,000 ppm 농도의 마늘 추출물이 S. aureus 생육에 있어서 유도기를 8시간까지 상당히 연장시켰다. Esherichia coli의 생육에 미치는 영향을 paper disc method로 측정한 생육저해환의 결과에서 대부분의 김치 재료 methanol 추출물이 우수한 항균성을 나타낸 것과 같이 농도별 생육 곡선을 측정한 결과에서도 미약하지만 모든 추출물에서 생육 억제 효과를 나타내었다. 최소저해농도(MIC)는 고춧가루 추출물이 L. monocytogenes에 대하여 500 $\mu$g/mL로 가장 낮은 값을 보였다.

Keywords

References

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