Journal of Periodontal and Implant Science

  • 제35권4호
  • /
  • Pages.1097-1108
  • /
  • 2005
  • /
  • 2093-2278(pISSN)
  • /
  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

이형상 칼슘 인산염(Biphasic Calcium Phosphate)에 의한 성체 줄기세포의 골아세포 분화

Osteoblastic differentiation of adult stem cells by Biphasic Calcium Phosphate

  • 이명구 (원광대학교 치과대학 치주과학 교실) ;
  • 임성우 (원광대학교 치과대학 치주과학 교실) ;
  • 피성희 (원광대학교 치과대학 치주과학 교실) ;
  • 김윤상 (원광대학교 치과대학 치주과학 교실) ;
  • 신형식 (원광대학교 치과대학 치주과학 교실) ;
  • 유형근 (원광대학교 치과대학 치주과학 교실)
  • Lee, Myoung-Ku (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Lim, Sung-Woo (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Pi, Sung-Hee (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Yun-Sang (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • Shin, Hyung-Shik (Department of Periodontology, School of Dentistry, Wonkwang University) ;
  • You, Hyoung-Keun (Department of Periodontology, School of Dentistry, Wonkwang University)
  • 발행 : 2005.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The present study was to determine the influence of micro-macro biphasic calcium phosphate(MBCP) on proliferation and differentiation of human marrow-derived mesenchymal stem cells. Primary stem cells were cultured from bone marrow and 3-4 passaged cells were used. This study tested the proliferative effects by cell counting. Collagen sythensis, alkaline phosphatase activity, expression of osteocalcin and bone sialoprotein by Western blot analysis were evaluated. The cellular proliferation of ASC was not influenced by MBCP. Collagen synthesis of ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The ALP activity in ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The expression of OC and BSP incresaed in ASC cultured on MBCP. These results suggest that MBCP may stimulates the osteoblastic activity of ASC.

키워드

참고문헌

  1. Schallhorn RG. Present status of osseous grafting procedures. J Periodontol. 1977:48(9):570-576 https://doi.org/10.1902/jop.1977.48.9.570
  2. Froum S.. Thaler R.. Scopp I.. Stahl S. Osseous autografts. II. Histological responses to osseous coagulum-bone blend grafts. J Periodontol. 1975:46:656-661 https://doi.org/10.1902/jop.1975.46.11.656
  3. Ray RD. Vascularization of bone grafts and implants. Clin Ortho. 1972;87:42-48
  4. Ennecking W.F., Morris J.L. Human autologous cortical bone transplants. Clin Ortho. 1972;87:28-35
  5. Schwartz Z., Somers A., Mellonig JT., Carnes DL Jr., Dean DD., Cochran DL., Boyan BD. Ability of commercial demineralized freeze-dried bone allograft to induce new bone formation is dependent on donor age but not gender. J Periodontol. 1998;69(4) :470-478 https://doi.org/10.1902/jop.1998.69.4.470
  6. Traianedes K., Russell JL., Edwards JT., Stubbs HA., Shanahan IR, Knaack D. Donor age and gender effects on osteoinductivity of demineralized bone matrix. J Biomed Mater Res. 2004;70B (1) :21-29 https://doi.org/10.1002/jbm.b.30015
  7. Richardson CR, Mellonig JT., Brunsvold MA., McDonnell HT., Cochran DL. Clinical evaluation of Bio-Oss: a bovinederived xenograft for the treatment of periodontal osseous defects in humans. J Clin Periodontol. 1999;26(7) :421-428 https://doi.org/10.1034/j.1600-051X.1999.260702.x
  8. Barney VC., Levin M.P., Adams DF. Bioceramic implants in surgical periodontal defects. A comparison study. J Periodontol. 1986;57(12) :764-770 https://doi.org/10.1902/jop.1986.57.12.764
  9. Bowers GM., Vargo JW., Levy B., Emerson JR, Bergquist JJ.. Histologic observations following the placement of tricalcium phosphate implants in human intrabony defects. J Periodontol. 1986; 57(5) :286-287 https://doi.org/10.1902/jop.1986.57.5.286
  10. Froum SJ., Kushner L., Scopp IW., Stahl SS. Human clinical and histologic responses to Durapatite implants in intraosseous lesions. Case reports. J Periodontol. 1982: 53(12): 719-725 https://doi.org/10.1902/jop.1982.53.12.719
  11. Moskow BS., Lubarr A. Histological assessment of human periodontal defect after durapatite ceramic implant. Report of a case. J Periodontol. 1983:54(8) :455-462 https://doi.org/10.1902/jop.1983.54.8.455
  12. Roy DM., Linnehan SK. Hydroxyapatite formed from coral skeletal carbonate by hydrothermal exchange. Nature. 1974: 247(438) :220-222 https://doi.org/10.1038/247220a0
  13. Gauthier O., Bouler JM., Aguado E., Pilet P.. Daculsi G. Micro-macro biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone growth. Biomaterials. 1998: 19: 133-139 https://doi.org/10.1016/S0142-9612(97)00180-4
  14. Holm S. Embryonic stem cell research and the moral status of human embryos. Reprod Biomed Online. 2005: 10 Suppl 1 :63-67 https://doi.org/10.1016/S1472-6483(10)62209-2
  15. Song L., Baksh D., Tuan RS. Mesenchymal stem cell-based cartilage tissue engineering: cells, scaffold and biology. Cytotherapy. 2004:6(6) : 596-601 https://doi.org/10.1080/14653240410005276-1
  16. Barry FP., Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol. 2004: 36(4): 568-584 https://doi.org/10.1016/j.biocel.2003.11.001
  17. Kassem M., Kristiansen M., Abdallah BM. Mesenchymal stem cells: cell biology and potential use in therapy. Basic Clin Pharmacol Toxicol. 2004:95(5) :209-214 https://doi.org/10.1111/j.1742-7843.2004.pto950502.x
  18. Friedenstein AJ., Petrakova KV., Kurolesova AI., Frolova GP. Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. 1968;6(2) :230-247 https://doi.org/10.1097/00007890-196803000-00009
  19. Ohgushi H.. Caplan AI. Stem cell technology and bioceramics: from cell to gene engineering. J Biomed Mater Res. 1999;48(6):913-927 https://doi.org/10.1002/(SICI)1097-4636(1999)48:6<913::AID-JBM22>3.0.CO;2-0
  20. Dunn MA .. Kamel R.. Kamel IA.. Biempica L.. Kholy AE.. Hait PK.. Rojkind M.. Warren KS.. Mahmoud M. Liver collagen synthesis in schistosomiasis mansoni. Gastroenterology. 1979;76(5 Pt 1): 978-982
  21. Barrack RL., Cook SD.. Patron LP.. Salkeld SL., Szuszczewicz E., Whitecloud TS. Induction of bone ingrowth from acetabular defects to a porous surface with OP-1. Clin Orthop Relat Res. 2003; (417) :41-59
  22. Gauthier O., Bouler JM., Aguado E., Pilet P., Daculsi G. Micro-macro biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone ingrowth. Biomaterials. 1998; 19(1-3): 133-139 https://doi.org/10.1016/S0142-9612(97)00180-4
  23. De Groot K. Effect of porosity and physicochemical properties on the stability, resorption, and strength of calcium phosphate ceramics. Ann N Y Acad Sci. 1988;523:227-233 https://doi.org/10.1111/j.1749-6632.1988.tb38515.x
  24. Gauthier O., Bouler JM.. Aguado E., Pilet P., Daculsi G. Micro-macro biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone ingrowth. Biomaterials. 1998; 19(1-3): 133-139 https://doi.org/10.1016/S0142-9612(97)00180-4
  25. Thomson JA.. ltskovitz-Eldor J.. Shapiro SS., Waknitz MA., Swiergiel JJ., Marshall VS., Jones JM. Embryonic stem cell lines derived from human blastocysts. Science. 1998: 282(5391) : 1145-1147 https://doi.org/10.1126/science.282.5391.1145
  26. Leung KS., Fung KP., Sher AH., Li CK., Lee KM. Plasma bone-specific alkaline phosphatase as an indicator of osteoblastic activity. J Bone Joint Surg Br. 1993:75(2) :288-292
  27. Delmas PD. Biological markers of bone metabolism. Presse Med. 1993 :22(6): 263-268
  28. van Straalen JP., Sanders E., Prummel MF., Sanders GT. Bone-alkaline phosphatase as indicator of bone formation. Clin Chim Acta. 1991:201(1-2):27-33 https://doi.org/10.1016/0009-8981(91)90021-4
  29. Duda RJ Jr., O'Brien JF., Katzmann JA., Peterson JM., Mann KG., Riggs BL. Concurrent assays of circulating bone Gla-protein and bone alkaline phosphatase: effects of sex, age, and metabolic bone disease. J Clin Endocrinol Metab. 1988:66(5):951-957 https://doi.org/10.1210/jcem-66-5-951
  30. Bowles SA., Kurdy N., Davis AM., France MW., Marsh DR. Serum osteocalcin, total and bone-specific alkaline phosphatase following isolated tibial shaft fracture. Ann Clin Biochem. 1996: 33(Pt 3):196-200 https://doi.org/10.1177/000456329603300304
  31. Marazuela M., Astigarraga B., Tabuenca MJ., Estrada J., Marin F., Lucas T. Serum bone Gla protein as a marker of bone turnover in acromegaly. Calcif Tissue Int. 1993:52(6) :419-421. https://doi.org/10.1007/BF00571329
  32. Bouman AA., Scheffer PG., Ooms ME., Lips P., Netelenbos C. Related Articles, Two bone alkaline phosphatase assays compared with osteocalcin as a marker of bone formation in healthy elderly women. Clin Chem. 1995:41(2): 196-199
  33. Shimizu-Sasaki E., Yamazaki M., Furuvama S., Sugiya H., Sodek J., Ogata Y. Identification of a novel response element in the rat bone sialoprotein(BSP) gene promoter that mediates constitutive and fibroblast growth factor 2-induced expression of BSP. J Biol Chem. 2001: 276: 5459-5466 https://doi.org/10.1074/jbc.M008971200
  34. Wuttke M. Muller S, Nitsche DP, Paulsson M. Hanisch FG, Maurer P. Structural characterization of human recombinant and bone-derived bone sialoprotein. Functional implications for cell attachment and hydroxyapatite binding. J Biol Chem. 2001:276:36839-36848 https://doi.org/10.1074/jbc.M105689200