Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Effect of BMP-7 on the rat periodontal ligament cell

치주인대세포에 대한 Bone morphogenetic protein-7의 영향

  • Kim, Kyung-Hee (Department of Periodontology, Dental Science Research Institute, Chonnam National University) ;
  • Kim, Young-Jun (Department of Periodontology, Dental Science Research Institute, Chonnam National University) ;
  • Chung, Hyun-Ju (Department of Periodontology, Dental Science Research Institute, Chonnam National University)
  • 김경희 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소) ;
  • 김영준 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소) ;
  • 정현주 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소)
  • Published : 2005.06.30
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Abstract

Bone morphogenetic protein-7(BMP-7), a member of the transforming growth factor superfamily, stimulates osteoblast differentiation and bone formation. There are lots of evidences supporting a direct participation of periodontal ligament(PDL) cells on periodontal tissue regeneration. The purpose of this study was to evaluate the effect of recombinant human(rh) BMP-7 on primary rat PDL cells in vitro, with special focus on the ability of bone formation. The PDL cells were cultured with rhBMP-7 at the concentration of 0, 10, 25, 50, 100 and 200ng/ml for MTT assay. We evaluated the alkaline phosphatase activity at 3 and 5 days of incubation and the ability to produce mineralized nodules of rat PDL cells at 14 days of cell culture in concentration of 0, 10, 25, 50 and 100ng/ml. The cell activity was not reduced in cells treated with BMP-7 at $10{\sim}100ng/ml$, whereas the cell activity was reduced in the concentration of 200ng/ml than the control at day 1 and 3(p<0.01). At 3 and 5 day, alkaline phosphatase activity was significantly increased in cells treated with BMP-7 at 50ng/ml and 100ng/ml(p<0.05). The area of mineralized bone nodule was greater in cells treated with BMP-7 at 50 and 100 ng/ml than the control(p<0.01). These results suggest that rhBMP-7 stimulate rat PDL cells to differentiate toward osteoblast phenotype and secretion of the extracellular matrix of rat PDL cells.

Keywords

References

  1. Melcher AH. On the repair potential of periodontal tissue. J Periodontol 1976:47:256-260 https://doi.org/10.1902/jop.1976.47.5.256
  2. Iglhaut J, Aukhil I, Simpson DM, Johnston MC, Kock G. Progenitor cell kinetics during tissue regeneration in experimental periodontal wounds. J Periodont Res 23:107-117 https://doi.org/10.1111/j.1600-0765.1988.tb01342.x
  3. McCulloch CAG. Progenitor cell populations in periodontal ligament of mice. Anat rec 1985;211:258-262 https://doi.org/10.1002/ar.1092110305
  4. Gould TRL, Melcher AH, Brunette DM. Migration and division of progenitor cell populations in the periodontal ligament after wounding. J Periodont Res 1980;15:20-42 https://doi.org/10.1111/j.1600-0765.1980.tb00258.x
  5. HerrY, Matsuda M. Lin WL. Genco RJ, Cho MI. The origin of fibroblast and their role in the early stages of horizontal furcation defects healing in the beagle dogs. J Periodontol 1995;66:716-730 https://doi.org/10.1902/jop.1995.66.8.716
  6. McCulloch CAG, Bordin S. Role of fibroblast subpopulations in periodontal physiology and pathology. J Periodont Res 1991;26:144-154 https://doi.org/10.1111/j.1600-0765.1991.tb01638.x
  7. Mellonig JT, Bowers GM. Bright RW, Lawlence JJ. Clinical evaluation of freeze-dried bone allografts in periodontal osseous defects. J Periodontol 1976;47:125-131 https://doi.org/10.1902/jop.1976.47.3.125
  8. Pitaru S, Tal H, Soldinger M, Noff M. Collagen membranes prevent apical migration of epithelium and support new connective tissue attachment during periodontal wound healing in dogs. J Periodontal Res 1989;24:247-253 https://doi.org/10.1111/j.1600-0765.1989.tb01789.x
  9. Ripamonti D, Reddish AH. Perio- dontal regeneration: potential role of bone morphogenic proteins. J Periodont Res 1994:29:225-235 https://doi.org/10.1111/j.1600-0765.1994.tb01216.x
  10. Matsuda N, Kumar M, Ramakrishnan PR. Lin WL, Genco RJ, Cho :MI. Evidence for upregulation of epidermal growth factors on rat periodontal ligament fibroblastic cells associated with stabilization of phenotype in vitro. Arch Oral Biol. 1993:38:559-569 https://doi.org/10.1016/0003-9969(93)90120-B
  11. Urist MR. Bone formation by autoindue- tion. Science 1965:150:893-899 https://doi.org/10.1126/science.150.3698.893
  12. Lee MB. Bone morphogenic proteins: background and implications for oral reconstruction. A review. J Clin Periodontol 1997;24:355-365 https://doi.org/10.1111/j.1600-051X.1997.tb00198.x
  13. Wozney JM, Rosen V. Bone morphogenic proteins and Bone morphogenic protein gene family in bone formation and repair. Clin Ortho Rel Res 1998;346:26-37
  14. Thies RS, Bauduy M, Ashton BA, Kurtzberg L, Wozney JM, Rosen V. Recombinant human bone morphogenic protein-2 induces osteoblastic differentiation in W-20-17 stromal cell. Endocrinology 1992;130:1318-1324 https://doi.org/10.1210/en.130.3.1318
  15. Kim DJ, Moon SH, Kim H, Kwon UH, Park MS, Han KJ, Hahn SB, Lee HM. Bone morphogenetic protein-2 facilitates expression of chondrogenic, not osteogenic, phenotype of human intervertebral disc cells. Spine. 2003;28:2679-2684 https://doi.org/10.1097/01.BRS.0000101445.46487.16
  16. Sigurdsson TJ, King N, Cruchley AT, Wozneyy JM, Hughes FJ Wikesjo UM. Periodontal repair in dogs: recombinant human bone morphogenetic protein-2 significantly enhances periodontal regeneration. J Periodontol 1995;66:131-138 https://doi.org/10.1902/jop.1995.66.2.131
  17. King GN, King N, Cruchley AT, Wozney JM, Hughes FJ. Recombinant human bone morphogenetic protein-2 promotes wound healing in rat periodontal fenestration defects. J Dent Res. 1997;76:1460-1470 https://doi.org/10.1177/00220345970760080801
  18. Wikesjo UM, Guglielmoni P, Promsudthi A, Cho KS, Trombelli L, Selvig KA, Jin L, Wozney JM, Periodontal repair in dogs: effect of rhBMP-2 concentration on regeneration of alveolar bone and perio- dontal attachment. J Clin Perio- dontol. 1999;26:392-400 https://doi.org/10.1034/j.1600-051X.1999.260610.x
  19. Wikesjo UM, Xiropaidis AV, Thomson RC, Cook AD, Selvig KA, Hardwick WR. Periodontal repair in dogs: rhBMP -2 significantly enhances bone formation under provisions for guided tissue regeneration. J Clin Periodontol. 2003;30:705-714 https://doi.org/10.1034/j.1600-051X.2003.00363.x
  20. Giannobile WV, Ryan S, Shih MS, Su DL, Kaplan PL, Chan TC. Recombinant human osteogenic protein- 1( OP-1) stimulates periodontal wound healing in class III furcation defects. J Periodontol. 1998;69:129-137 https://doi.org/10.1902/jop.1998.69.2.129
  21. Raishankar D, McCulloch CA Te- nen-baum HC, Lekic PC. Osteogenic inhibition by rat periodontal ligament cells: modulation of bone morphogenic protein-7 activity in vivo. Cell Tissue Res. 1998;294:475-483 https://doi.org/10.1007/s004410051199
  22. King GN, Cochran DL. Factors that modulate the effects of bone morphogenetic protein-induced periodontal re- generation: a critical review. J Periodontol. 2002;73:925-36 https://doi.org/10.1902/jop.2002.73.8.925
  23. Chen TL. Shen WJ. Kraemer FB.Human BMP-7/0P-1 induces the growth and differentiation of adipocytes and osteoblasts in bone marrow stromal cell cultures. J Cell Biochem. 2001;82:187-199 https://doi.org/10.1002/jcb.1145
  24. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J lmmunol Methods 1983;65:55 -63 https://doi.org/10.1016/0022-1759(83)90303-4
  25. Anusaksathien O. Giannobile WV. Growth factor delivery to re-engineer periodontal tissues. Curr Pharmaceut Biotechn. 2002:3:129-139 https://doi.org/10.2174/1389201023378391
  26. Taira M. Nakao H. Takahashi J. Araki Y. Effects of two growth factors and dexamethasone on the proliferation of rat bone marrow stromal cells and osteoblastic MC3T3-E1 cells. J Oral Rehabilitation. 2003:30:697-701 https://doi.org/10.1046/j.1365-2842.2003.01118.x
  27. Roach HI. Why does bone matrix contain non-collagenous proteins? The possible roles of osteocalcin, osteonectin, osteopontin and bone sialoprotein in bone mineralization and resorption. Cell Biol Int 1994;18:617-628 https://doi.org/10.1006/cbir.1994.1088
  28. Franceschi RT. The developmental control of osteoblast-specific gene expression. Role of specific transcription factors and the extracellular matrix environment. Crit Rev Oral Biol Med 1999;10:40-57 https://doi.org/10.1177/10454411990100010201
  29. Asahina I. Sampath TK. Hauschka PV. Human osteogenic protein-I induces chondroblastic. osteoblastic, and/or adipocytic differentiation of clonal murine target cells. Exp Cell Res 1996;222:38-47 https://doi.org/10.1006/excr.1996.0005
  30. Yeh LC, Adamo ML, Olson MS, Lee JC. Osteogenic protein-I and insulin- like growth factor I synergistically stimulate rat osteoblastic cell differentiation and proliferation. Endocrinology. 1997;138:4181-4190 https://doi.org/10.1210/en.138.10.4181
  31. Notoya K. Tsukuda R. Yoshida K. Taketomi S. Stimulatory effect of ipriflavone on formation of bone-like tissue in rat bone marrow stromal cell culture. Calcif Tissue Int 1992:51:S16-20 https://doi.org/10.1007/BF02180244
  32. Notoya K, Yoshida K, Tsukuda R, Taketomi S, Effect of ipriflavone on expression of markers characteristic of the osteoblast phenotype in rat bone marrow stromal cell culture. J Bone Miner Res 1994;9:395-400
  33. Li WS, Cheifetz S, McCulloch CAG, Sampath K. Sodek J. Effects of osteogenic protein-Ion bone matrix protein expression by fetal rat calvarial cell are differentiation stage specific. J Cell Phy- siol 1996:169:115-125 https://doi.org/10.1002/(SICI)1097-4652(199610)169:1<115::AID-JCP12>3.0.CO;2-C