Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Inhibition of MMP-13 mRNA expression by ginseng saponin in fetal rat calvarial cells

백서 태자 두개관세포에서 인삼 사포닌에 의한 MMP-13 mRNA 발현 억제

  • Kim, Yang-Yi (Dept. of Periodontology, Chonnam National University) ;
  • Ciu, De-Zhe (Graduate School, Dept. of Dental Science, Chonnam National University, Dental Science Research institute, Chonnam National University) ;
  • Kim, Young-Joon (Dept. of Periodontology, Chonnam National University, Dental Science Research institute, Chonnam National University)
  • 김양이 (전남대학교 치과대학 치주과학교실) ;
  • 최득철 (전남대학교 대학원 치의학과, 전남대학교 치의학연구소) ;
  • 김영준 (전남대학교 치과대학 치주과학교실, 전남대학교 치의학연구소)
  • Published : 2005.06.30
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Abstract

There is a potential role of collagenase-3 in alveolar bone loss and periodontal disease progression, we need to develope or find chemotherapeutic drugs or herbal agents which may regulate the expression of MMP-13. Ginseng saponin, one of the major components of Korea ginseng(panax ginseng) root, has many various biologic effects, such as cytotoxic effect, tumoricidal effects, cytokine regulations, and protein biosynthesis effect. The purpose of this study was to determine the effects of Korea red ginseng saponin on MMP-13 gene expression in osteoblasts. The experimental groups were cultured with ginseng saponin in concentration of 1.0, 10, 25, 50, 100, 250 and $500{\mu}g/ml$ for MTT assay. Primary rat calvarial cells were pre-treated for 1 hour with ginseng saponin(100 ${\mu}g/ml$) and then stimulated with $IL-1{\beta}(1.0ng/ml)$ and PTH(10 nM). MMP-13 gene expression was evaluated by RT-PCR. The results were as follows: Ginseng saponin was cytotoxic to osteoblast at concentration exceeding $250{\mu}g/ml$ for longer than 24 hours in tissue culture(p<0.01). In RT-PCR analysis, steady state MMP-13 mRNA levels were increased approximately 350% by $IL-1{\beta}$, and 400% by PTH when normalized to untreated control. $IL-1{\beta}-indued$ MMP-13 mRNA expression was reduced 50% by pretreatment with ginseng saponin. But ginseng saponin didn't inhibit MMP-13 expression from PTH stimulated cells. This results suggest that ginseng saponin Inhibit $IL-1{\beta}-indued$ MMP-13 mRNA expression.

Keywords

References

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