Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effect of Culture Medium, Temperature and Coculture on Culture of Immature Porcine Spermatogonia Cells

미성숙 돼지 정조세포 배양에 미치는 배양액, 배양온도 및 공배양 효과

  • Kim H. S. (National Livestock Research Institute) ;
  • Cho S. R. (National Livestock Research Institute) ;
  • Choi S. H. (National Livestock Research Institute) ;
  • Han M. H. (National Livestock Research Institute) ;
  • Son D. S. (National Livestock Research Institute) ;
  • Ryu I. S. (National Livestock Research Institute) ;
  • Kim I. C. (National Livestock Research Institute) ;
  • Lee J. H. (National Livestock Research Institute) ;
  • Kim I. H. (Chungbuk National University) ;
  • Im K. S. (Seoul National University)
  • 김현종 (축산연구소 가축유전자원시험장) ;
  • 조상래 (축산연구소 가축유전자원시험장) ;
  • 최선호 (축산연구소 가축유전자원시험장) ;
  • 한만희 (축산연구소 가축유전자원시험장) ;
  • 손동수 (축산연구소 가축유전자원시험장) ;
  • 류일선 (축산연구소 가축유전자원시험장) ;
  • 김인철 (축산연구소 가축유전자원시험장) ;
  • 이장희 (축산연구소 가축유전자원시험장) ;
  • 김일화 (충북대학교 수의학과) ;
  • 임경순 (서울대학교 동물자원과학과)
  • Published : 2005.04.01
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Abstract

This study was carried out for development of effective preservation on animal genetic resources. Spermatogonia cells are the germline stem cells and they can be restored to adult animal with proliferation and differentiation intentionally. When the spermatogonia cells were purified from seminiferous tubules and were cultured at $32^{\circ}C$, the cells were actively proliferated. The culture medium consisted of TCM199 plus $10\%$ FCS and coculture with Sertoli cells supported cultivation of spermatogonia cells. By passing 40 days of incubation, spermatogonia cells formed the germline colony or shape of ES-like colony or reconstruction of pseudo-seminifcrous tubule shape. At 40 days, the cultured cells were no sign for differentiation to spermatocyte or spermatid. The experiment of induced differentiation of this cells is needed.

본 연구는 가축유전자원의 효율적 보존을 위해 정조세포를 줄기세포 형태로 장기보관하면서 필요에 따라 증식, 분화를 통해 가축의 복원에 활용하기 위한 연구의 일부로 진행되었다. 정조세포를 분리하여 배양한 결과 배양온도는 다른 세포들과는 달리 $32^{\circ}C$에 세포분열이 활발하였으며, TCM199에 $10\%$ FCS를 첨가한 배양액과 세르톨리세포 공배양으로 정조세포의 배양을 지지하였다. 40일령이 지나면서 정조세포 콜로니 즉 germline stem cells를 형성하였으며, 일부에서는 외형상 ES-like cells를 형성하거나, 세정관 형태로 정조세포들이 재구성되었다. 40일령까지 배양한 상태에서는 정조세포의 정모세포나 정자세포로 분화하는 징후를 관찰할 수 없었으며, 추후 이들 세포로 분화를 유기하는 실험이 진행되어야 할 것이다.

Keywords

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