DOI QR코드

DOI QR Code

Characterization of the White Spot Syndrome Baculovirus (WSBV) Infection In Fresh Shrimp, Penaeus chinensis, Cultured in Korea

한국의 양식대하에서의 흰반점증상 바이러스감염의 특징

  • Heo Moon-Soo (Faculty of Marine Science, Cheju National University)
  • Published : 2005.04.01

Abstract

The virions of causative virus for white spot syndrome in cultured Fresh shrimp, Penaeus chinensis were rod-shaped, double envelope. An average size of the virion was 70 nm in diameter and $250\~300$ nm in length. Histopathological test of affected stomach, heart, and lymphoid organ revealed nuclear hypertrophy. Infectivity trials carried out by injection and feeding with purified virus revealed high cumulative mortality to healthy shrimp. The twenty one different protein species were detected in the analysis of virion. The length of total DNA from the purified virus particles were detected as a single band, double-stranded DNA molecule of approximately 114 kb.

대하 새우양식장의 대하에 흰반점 증상을 나타내는 원인바이러스는 막대형의 이중막을 가지고 있었으며, 전자 현미경 관찰 결과 평균 크기가 $250\~300\times70\;nm$였고, 조직학적 병변은 위상피 등에서 핵이 비대해지는 것이 관찰되었다. 공격실험에서는 건강체의 새우에 많은 누적 폐사율을 보였다. 원인 바이러스 단백질은 21개의 밴드를 보였으며, 핵산 분석 결과 total 분자량은 114 kb로 나타났다.

Keywords

References

  1. Bell T. A. and Lightner D. V. 1988. A Handbook of Normal Penaeid Shrimp Histology. World Aquaculture Society, Baton Rouge, LA
  2. Haung, J. X., Y. J. Song and C. Yang. 1994. Baculoviral hypodermal and hematopoietic necrosis pathology of the shrimp explosive epidemic disease. Yellow Sea Fishery Research Institute. Qingdao. China. 16. 1-10
  3. Heo, M.S. 1997. Viral disease of the cultured Penaeus chinensis and Penaeus japonicus. Ph.D. Thesis, Pusan National University. 10-27
  4. Inouye, K, S. Miwa, N. Oseko, H. Nakano, T.Kimura, K Momoyama and M. Hiraoka. 1994. Mass mortalities of cultured kuruma shrimp, Penaeus iaponicus, in Japan in 1993: Electron microscopic evidence of the causative virus. Fish Pathol., 29, 149-158 https://doi.org/10.3147/jsfp.29.149
  5. Inouye, K., K. Yamane, N. Ikeda, T. Kimura, H. Nakano, K. Momoyama, J. Kobayashi and S. Miyagima. 1996. The penaeid rod-shaped DNA virus (PRDV), which causes Penaeus Acute Viremia (PAV). Fish Pathol., 31, 39-45 https://doi.org/10.3147/jsfp.31.39
  6. Kim, C. K, P. K Kim, S. G. Sohn, D. S. Sim, M. A. Park, M. S. Heo, T .H. Lee, H. K Jun. and K .L. Jang. 1998. Development of a polymerase chain reaction (PCR) procedure for detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimp. Journal of Fish Diseases, 21, 11-17 https://doi.org/10.1046/j.1365-2761.1998.00324.x
  7. Laemmli UK 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680-685 https://doi.org/10.1038/227680a0
  8. Momoyama, K, M. Hiraoka, H. Nakano, K Koube, K Inouye and N. Oseko. 1994. Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: Histopathological study. Fish Pathol., 29, 141-14 https://doi.org/10.3147/jsfp.29.141
  9. Reynolds, E. S. 1993. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol., 17, 208-212 https://doi.org/10.1083/jcb.17.1.208
  10. Sambrook, J., Fritsch. E. F. and T. Maniatis. 1989. Molecular Clonig. A Laboratory Manual. 2nd ed. New York, Cold Spring Harbor Pressl
  11. Spurr, A. S. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res., 26, 31-34 https://doi.org/10.1016/S0022-5320(69)90033-1
  12. Takahashi, Y., T. Itami, M. Kondo, M. Maeda, R. Fujii, S. Tomonaga, K Supamattaya and S. Boonyaratpalin. 1994. Electron microscopic evidence of bacilliform virus infection in kuruma shrimp (Penaeus japonicus). Fish Pathol., 29, 121-125 https://doi.org/10.3147/jsfp.29.121
  13. Wang, C. H., Lo. C .H., Leu. J .H., Chou. C. M., Yeh. P. Y., Tung. M. C, Chang. C. F., Suo M .S., Kou. G .H. 1995. Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Dis. aquatic Org. 23, 239-242 https://doi.org/10.3354/dao023239
  14. Wongteerasupaya C., Vickers J. E., Sriurairatana S., Nash G. L., Akarajamorn A., Boonsaeng V., Panyim S., Tassanakajon A., Withyachumnarnkul B. and Flegel T. W. 1995. A nonoccluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. Dis. aquatic Org. 21, 69-77 https://doi.org/10.3354/dao021069
  15. Zheng, G., Yalin. S. Kai, Z. and Cao. Z. 1997. Bacilliform virus infection in cultured Chinese shrimp, Penaeus orientalis, in China. J Mar Biotechnol. 5, 113-118

Cited by

  1. The Study of Genetic Diversity and Population Structure of the Korean Fleshy Shrimp, Fenneropenaeus chinensis, Using Newly Developed Microsatellite Markers vol.25, pp.12, 2015, https://doi.org/10.5352/JLS.2015.25.12.1347