Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Cytokinin and Putrescine on Plant Regeneration from Leaf Explant of Rhodiola sachalinesis A. Bor

홍경천 (Rhodiola sachalinensis A. Bor) 잎절편으로부터 식물체 재분화에 미치는 Cytokinin과 Putresine의 영향

  • Bae Ki-Hwa (Korea Ginseng Institute, Chung-Ang University) ;
  • Lim Soon (Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KIRBB)) ;
  • Yoon Eui-Soo (Department of Biological Science, Kong-ju National University) ;
  • Shin Cha-Gyun (Korea Ginseng Institute, Chung-Ang University) ;
  • Kim Yoon-Young (Korea Ginseng Institute, Chung-Ang University) ;
  • Kim Yun-Soo (Korea Ginseng Institute, Chung-Ang University)
  • 배기화 (중앙대학교 인삼산업연구소) ;
  • 임순 (한국생명공학연구원 식물세포공학연구실) ;
  • 윤의수 (공주대학교 생물학과) ;
  • 신차균 (중앙대학교 인삼산업연구소) ;
  • 김윤영 (중앙대학교 인삼산업연구소) ;
  • 김윤수 (중앙대학교 인삼산업연구소)
  • Published : 2005.09.01
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Abstract

The effects of cytokinin and putrescine on adventitious shoot induction from leaf explant of Rhodiola sachalinensis A. Bor were investigated. Among cytokinin used in this experiment, BA was more effective on adventitious shoot induction and shoot elongation than kinetin. Especially, 1 mg/L BA was the best to increase adventitious shoot induction (71%) and shoot elongation (3.0 mm). In addition, 100 mM putrescine in MS medium with 1 mg/L BA was higher in adventitious shoot induction (93%) and shoot elongation (3.8 mm) than single treatment of 1 mg/L BA. Adventitious shoots induced in this experiment rooted on 1/2 MS medium and acclimated over 95% on composed soil (peatmoss:sand=1:1).

홍경천(Rhodilola sachalinensis A. Bor)의 잎절편로부터 부정아를 유도하고자 BA와 kinetin을 처리한 결과, BA의 처리가 kinetin의 처리보다 부정아 유도와 신초의 생장에 양호한 것으로 나타났으며, 특히 BA 1 mg/L처리에서 부정아 유도빈도와 신초길이는 각각 71%와 3.0 mm로 가장 높게 나타났다. 또한 고빈도의 부정아를 유도하고자 다양한 농도의 putrescine를 처리한 결과, putrescine 100 mM 처리에서 부정아 유도빈도가 BA 1 mg/L 단용처리보다 약 20%가 증가되었고 신초길이 역시 약 10 mm가 증가된 것으로 나타났다. 이상의 결과에서 유도된 부정아는 생장조절물질이 포함되지 않은 1/2MS배지에서 발근과 신장이 정상적으로 이루어졌으며 약 7 cm 정도 생장한 유식물체는 peatmoss:sand가 1:1로 혼합된 상토에서 약 95%이상 정상적으로 순화되었다.

Keywords

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