Journal of Acupuncture Research
- 제22권3호
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- Pages.215-225
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- 2005
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
Oligonucleotide chip를 이용한 홍화자약침액(紅花子藥鍼液)이 간암세포주(肝癌細胞柱)의 유전자(遺傳子) 발현(發顯)에 미치는 영향(影響)
Effect of Carthami Tinctorii Fructus Herbal-acupuncture Solution(CTF-HAS) on Gene Expression in HepG2 carcinomar cells
- 이경민 (대구한의대학교 한의과대학 침구경혈학교실) ;
- 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
- 정태영 (대구한의대학교 한의과대학 침구경혈학교실) ;
- 서정철 (대구한의대학교 한의과대학 침구경혈학교실) ;
- 한상원 (대구한의대학교 한의과대학 침구경혈학교실)
- Lee, Kyung-min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
- Lim, Seong-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
- Jung, Tae-young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
- Seo, Jung-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
- Han, Sang-won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University)
- 투고 : 2005.05.09
- 심사 : 2005.05.14
- 발행 : 2005.06.20
초록
홍화자약침액(紅花子藥鍼液)의 항암효능(抗癌效能)을 밝히고자 간암세포주(肝癌細胞柱)에 최신 oligonucleotide chip assay 법을 통하여 대양(大量)의 유전자(遺傳子) 발현(發顯)을 분석(分析)한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 1. MTT 분석(分析)에서 간암(肝癌) 및 위암세포주(胃癌細胞柱)는 홍화자약침액(紅花子藥鍼液) 1.5, 10, 20m/
Objective : It has long been known about the osteogenic effect of CTF-HAS on bone tissues. However, it has not been determined the effect of CTF-HAS on cancer cells. The purpose of this study is to screen the CTF-HAS mediated differentially expressed genes in cancer cells such as HepG2 hepatoma cells lines. Oligonucleotide microarray approach were employed to screen the differential expression genes. Methods : CTF-HAS was prepared by boiling and stored at