Clinical and Experimental Pediatrics

The Korean Pediatric Society (대한소아청소년과학회)
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Effects of Hyperosmolar Stimuli on Activation of Human Eosinophilic Leukaemia EoL-1 Cells

고삼투압 자극이 호산구의 활성화에 미치는 영향

  • Kwon, Byoung Chul (Department of Pediatrics, Yonsei University College of Medicine) ;
  • Kim, Eun Soo (Department of Pediatrics, Yonsei University College of Medicine) ;
  • Kim, Kyung Won (Department of Pediatrics, Yonsei University College of Medicine) ;
  • Song, Tae Won (Department of Pediatrics, Yonsei University College of Medicine) ;
  • Sohn, Myung Hyun (Department of Pediatrics, Yonsei University College of Medicine) ;
  • Shin, Myeong Heon (Department of Parasitology,Yonsei University College of Medicine) ;
  • Kim, Kyu-Eam (Department of Pediatrics, Yonsei University College of Medicine)
  • 권병철 (연세대학교 의과대학 소아과학교실) ;
  • 김은수 (연세대학교 의과대학 소아과학교실) ;
  • 김경원 (연세대학교 의과대학 소아과학교실) ;
  • 송태원 (연세대학교 의과대학 소아과학교실) ;
  • 손명현 (연세대학교 의과대학 소아과학교실) ;
  • 신명헌 (연세대학교 의과대학 기생충학교실) ;
  • 김규언 (연세대학교 의과대학 소아과학교실)
  • Received : 2005.03.17
  • Accepted : 2005.05.09
  • Published : 2005.08.15
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Abstract

Purpose : Airway dehydration and subsequent hyperosmolarity of periciliary fluid are considered critical events in exercise-induced bronchoconstriction. The aim of this study was to establish if a hyperosmolar challenge could induce activation of eosinophils. Methods : Human eosinophilic leukaemic cell lines, EoL-1 cells were incubated with hyperosmolar solutions for 15 minutes. Activation of EoL-1 cells was monitored by degranulation and superoxide anion production. In addition, we examined surface expression of CD69 and ICAM-1. Results : Hyperosmolar stimuli didn't induce superoxide anion production and degranulation. In addition, EoL-1 cells cultured with hyperosmolar medium at 930 mOsm/kg $H_2O$ resulted in no significant increment in fluorescent intensity of CD69 and ICAM-1 expression compared with results for cells incubated with isomolar medium. Conclusion : We found that hyperosmolar stimuli don't cause activation of EoL-1 cells, but further studies are required to determine the role of eosinophil in the mechanism of exercise-induced asthma.

목 적 : 운동 중 과호흡으로 인해 기도 내에 열과 수분이 손실되어 기도 내의 삼투압이 높아지게 되고, 이로써 여러 세포들이 염증 매개체를 유리함으로써 운동 유발성 천식을 일으킨다고 추정하고 있다. 이에 본 연구에서는 알레르기 염증반응에 중요한 역할을 하는 호산구에 고삼투압으로 자극시킨 후 호산구의 활성화 여부를 알아보고자 하였다. 방 법 : 호산구 세포주인 EoL-1 세포에 각각의 삼투질 농도와 시간에 따른 superoxide anion의 생성을 측정하였고, EoL-1 세포에 15분 동안 고삼투압 자극(930 mOsm/kg $H_2O$)을 준 직후와 이후 등장성 배지에 옮겨 5시간 동안 배양 후 유세포 분석기를 이용하여 호산구 표면의 CD69와 ICAM-1의 발현을 측정하였고, 배양 상층액에서 ECP 농도를 측정하였다. 결 과 : EoL-1 세포에 15분간 고삼투압 자극을 준 직후 측정한 ECP 농도와 CD69 및 ICAM-1의 fluorescent intensity는 등장성 배지에 노출시켰을 경우와 비교하여 차이를 보이지 않았으며, 고삼투압 자극 후 등장성 배지에 5시간 동안 배양하였을 때에도 유의한 차이를 보이지 않았다. Superoxide anion의 생성에 있어서도 다양한 삼투질 농도에 따른 차이를 보이지 않았으며, 시간의 경과에도 차이를 보이지 않았다. 결 론 : 이번 연구에서는 운동 후 발생하는 기관지 수축의 조기 반응과 후기 반응과 관련하여 고삼투압 자극에 의한 호산구의 활성화를 관찰할 수 없었으나, 향후 연구에 있어 운동 유발성 천식과 관련한 호산구의 역할에 대한 많은 실험과 연구가 필요 하리라 사료된다.

Keywords

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