한국어병학회지 (Journal of fish pathology)

  • 제18권2호
  • /
  • Pages.147-156
  • /
  • 2005
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

한국산 해조류의 어류병원성세균에 대한 항균활성

Antimicrobial Activities of Korean Marine Algae against Fish Pathogenic Bacteria

  • 강소영 (여수대학교 수산해양대학 수산생명의학과) ;
  • 오명주 (여수대학교 수산해양대학 수산생명의학과) ;
  • 신종암 (여수대학교 수산해양대학 수산생명과학부 양식생물학전공)
  • Kang, So-Young (Department of Aqualife medicine, Yosu National University) ;
  • Oh, Myung-Joo (Department of Aqualife medicine, Yosu National University) ;
  • Shin, Jong-Ahm (Division of Aqualife Science, Yosu National University)
  • 발행 : 20050800
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 주요 어병세균인 Staphylococcus sp., Streptococcus sp., E. tarda 및 V. anguillarum에 대한 30 종의 해조류 추출물의 항균활성을 검색하였다. 그 결과, C. officinalis, D. simplex, G. furcata, G. lanceolata 및 G. turuturu의 경우는 그람 양성균인 Staphylococcus sp.에 대하여, 그리고 S. thunbergii 및 P. morrowii 는 그람 음성균인 E. tarda와 V. anguillarum에 대하여 항균활성을 나타냄을 밝혔다. 그 중, 가장 우수한 항균활성을 나타낸 P. morrowii의 항균활성분획물인 90% MeOH 분획물은 E. tarda의 증식을 매우 효과적으로 저해하며, MIC는 1 mg/ml으로 측정되었다. 뿐만 아니라, 다양한 pH 조건하에서도 항균활성이 유지되었으며 분획물의 주요활성 성분은 할로겐 함유 phenol계 화합물일 것으로 추정된다.

To obtain antimicrobial algae against fish pathogenic bacteria, we screened 80% methanolic extracts of 30 algae using fish pathogenic bacteria, Staphylococcus sp., Streptococcus sp., Edwardsiella tarda and Vibrio anguillarum. Among them, Corallina officinalis, Dumontia simplex, Gloipeltis furcata, Grateloupia lanceolata and Grateloupia turuturu were effective for growth inhibition of a Gram-positive bacterium, Staphylococcus sp.. Sargassum thunbergii and Polysiphonia morrowii exhibited significant inhibitory effects against the growth of Gram-negative bacteria, both E. tarda and V. anguillarum. Moreover, antimicrobial activity-guided fractionation for P. morrowii extract yielded significantly active 90% methanolic fraction. This fraction significantly inhibited the growth of E. tarda exhibiting a MIC of 1 mg/ml. In addition, its antimicrobial activity was stable under various pH conditions.

키워드

참고문헌

  1. Ali, M. S., Saleem, M., Yamdagni, R. and Ali, M. A.: Steroid and antibacterial steroidal glycosides from marine green alga Codium iyen-garii Borgesen. Nat. Prod. Lett., 16: 407-413,2002 https://doi.org/10.1080/10575630290034249
  2. Asai, A., Sugawara, T., Ono, H. and Nagao, A.: Biotransformation of fucoxanthinol into amarouchiaxanthin a in mice and HepG2 cells: formation and cytotoxicity of fucoxan-thin metabolites. Drug Metab. Dispos., 32: 205-211,2004 https://doi.org/10.1124/dmd.32.2.205
  3. Awad, N. E., Selim, M. A., Saleh, M. M. and Mat-loub A. A.: Seasonal variation of the lipoidal matters and hypolipidaemic activity of the red alga Corallina officinalis L. Phytother. Res., 17: 19-25,2003 https://doi.org/10.1002/ptr.1005
  4. Bennamara, A., Abourriche, A., Berrada, M., Char-rouf, M., Chaib, N., Boudouma, M. and Gar-neau, F. X.: Methoxybifurcarenone: an antifungal and antibacterial meroditerpenoid from the brown alga Cystoseira tamariscifolia. Phytochemistry, 52: 37-40,1999 https://doi.org/10.1016/S0031-9422(99)00040-0
  5. Choi, J. S., Park, H. J., Jung, H. A., Chung, H. Y, Jung J. H. and Choi, W. C: A cyclohexa-nonyl bromophenol from the red alga Sym-phyocladia latiuscula. J. Nat. Prod., 63: 1705-1706, 2000 https://doi.org/10.1021/np0002278
  6. Enoki, N., Ishida, R., Urano, S., Ochi, M., Toko-royama, T and Matsumato, T.: New hydrozulenoid diterpenes from the marine alga Dictyota dichotoma. Chem. Lett., 1749-1752,1982
  7. Jork, H., Funk, W., Fischer, W. and Wimmer, H.: Thin-layer chromatography: reagents and detection methods. Vol.lb, pp. 227-231, VCH, Weinheim, Germany, 1994
  8. Kubo, I., Himejima, M., Tsujimoto, K., Muroi, H. and Ichikawa, N.: Antibacterial activity of crinitol and its potentiation. J. Nat. Prod., 55: 780-785, 1992 https://doi.org/10.1021/np50084a012
  9. Kurata, K. and Amiya, T.: A new bromophenol from red alga Polysiphonia urcerolata. Bull. Chem. Soc. Jpn., 53: 2020-2022,1980a https://doi.org/10.1246/bcsj.53.2020
  10. Kurata, K. and Amiya, T.: Bis(2,3,6-tribromo-4,5-dihydroxybenzyl)ether from the red alga, Symphyocladia latiuscula. Phytochemistry, 19: 141-142,1980b https://doi.org/10.1016/0031-9422(80)85032-1
  11. Kurihara, H., Mitani, T., Kawabata, T. and Taka-hashi, K.: Inhibitory potencies of bromophe-nols from rhodomelaceae algae against alpha-glucosidase activity. Fisheries Sci., 65: 300-303,1999 https://doi.org/10.2331/fishsci.65.300
  12. Langfield, R. D., Scarano, F. J., Heitzman, M. E., Kondo, M., Hammond, G. B. and Neto, C. C: Use of a modified microplate bioassay method to investigate antibacterial activity in the Peruvian medicinal plant Peperomia galioides. J. Ethnopharmacol., 94: 279-281, 2004 https://doi.org/10.1016/j.jep.2004.06.013
  13. Lim, C-W., Lee, J. S. and Cho, Y. J.: Structure and some properties of the antimicrobial compounds in the red alga, Symphyocladia latiuscula. J. Korean Fish Soc. 33: 280-287, 2000
  14. Liu, J. N., Yoshida, Y, Wang, M. Q., Okai, Y. and Yamachita, U.: B cell stimulating activity of seaweed extracts. Int. J. Immunopharmac., 19: 135-142,1997 https://doi.org/10.1016/S0192-0561(97)00016-7
  15. Ma, W. C. J., Chung, H. Y, Ang, Jr. P. O. and Kim, J-S.: Enhancement of bromophenol level in aquaculture silver seabream (Sparus sarba). J. Agric. Food Chem., 53: 2133-2139,2005 https://doi.org/10.1021/jf048390m
  16. Mayer, A. M. S. and Hamann, M. T.: Marine pharmacology in 1999: compounds with antibacterial, anticoagulant, antifungal, anthelmintic, anti-inflammatory, antiplatelet, antiprotozoal and antiviral activities affecting the cardiovascular, endocrine, immune and nervous systems, and other miscellaneous mechanisms of action. Comp. Biochem. Physiol. Part C, 132: 315-339, 2002 https://doi.org/10.1016/S1096-4959(02)00009-X
  17. Mayer, A. M. S. and Hamann, M. T.: Marine pharmacology in 2000: Marine compounds with antibacterial, anticoagulant, antifungal, anthelmintic, anti-inflammatory, antiplatelet, antiprotozoal and antiviral activities affecting the cardiovascular, endocrine, immune and nervous systems, and other miscellaneous mechanisms of action. Mar. Biotechnol. 6: 37-52,2004 https://doi.org/10.1007/s10126-003-0007-7
  18. Naviner, M., Berge, J. P., Durand, P. and Le Bris, H.: Antibacterial activity of the marine diatom Skeletonema costatum against aqua-cultural pathogens. Aquaculture, 174: 15-24, 1999 https://doi.org/10.1016/S0044-8486(98)00513-4
  19. Park, K-H., Oh, M-J. and Kim, H-Y.: Disinfection effect of chlorine dioxide on pathogenic bacteria from marine fish. J. Aquacult., 16: 118-123, 2003
  20. Saeki, Y, Kato, T. and Okuda, K.: Inhibitory effects of funoran on the adherence and colonization of oral bacteria. Bull. Tokyo Dent. Coll., 37: 77-92,1996
  21. Seo, Y, Lee, H. J. and Park, K. E.: Peroxynitrite-scavenging constituents from the brown alga Sargassum thunbergii. Biotech. Bioproc. Eng., 9: 212-216, 2004 https://doi.org/10.1007/BF02942295
  22. Vairappan, C. S., Kawamoto, T., Miwa, H. and Suzuki, M.: Potent antibacterial activity of halogenated compounds against antibiotic-resistant bacteria. Planta Med., 70: 1087-1090,2004 https://doi.org/10.1055/s-2004-832653
  23. Wang, W., Okada, Y, Shi, H., Wang, Y and Okuyama T.: Structure and aldose reductase inhibitory effects of bromophenol from the red alga Symphyocladia latiuscula. J. Nat. Prod., 68: 620-622, 2005 https://doi.org/10.1021/np040199j
  24. Whitfield, F. B, Helidoniotis, F. and Smith, D.: Role of feed ingredients in the bromophenols content of cultured prawns. Food Chem., 79: 355-365, 2002 https://doi.org/10.1016/S0308-8146(02)00187-5
  25. Xu, N., Fan, X., Yan, X., Li, X., Niu, R. and Tseng, C. K.: Antibacterial bromophenols from the marine red alga Rhodomela confervoides. Phytochemistry, 62: 1221-1224,2003 https://doi.org/10.1016/S0031-9422(03)00004-9
  26. Zhuang, C., Itoh, H. and Mizuno, T: Antitumor active fucoidan from the brown seaweed, umitoranoo (Sargassum thunbergii). Biosci. Biotech. Biochem., 59: 563-567,1995 https://doi.org/10.1271/bbb.59.563
  27. 우원식, 개정판 천연물화학연구법. 서울대학교출판부, p.14, 2002