Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Effect of Mother Trees and Dark Culture Condition Affecting on Somatic Embryogenesis of Liriodendron tulipifera L.

백합나무(Liriodendron tulipifera L.) 체세포배(體細胞胚) 발생(發生)에 미치는 모수(母樹) 및 암배양(暗培養) 효과(效果)

  • Son, Seog-Gu (Warm-temperature Forest Research Center, Korea Forest Research Institute) ;
  • Moon, Heung-Kyu (Div. Biothechnology, Korea Forest Research Institute) ;
  • Kim, Yong-Wook (Div. Biothechnology, Korea Forest Research Institute) ;
  • Kim, Ji-Ah (Div. Biothechnology, Korea Forest Research Institute)
  • 손석규 (국립산림과학원 난대산림연구소) ;
  • 문흥규 (국립산림과학원 생물공학과) ;
  • 김용욱 (국립산림과학원 생물공학과) ;
  • 김지아 (국립산림과학원 생물공학과)
  • Received : 2005.01.11
  • Accepted : 2005.02.21
  • Published : 2005.03.31
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Abstract

An effective micropropagation system for Liriodendron tulipifera L via somatic embryogenesis was established using immature seeds. Immature seeds from five individual trees were bisected longitudinally and cultured on two basal media (MS and B5) containing different combinations of 2,4-D and TDZ to induce callus and embryogenic tissue under light ($40{\mu}mol\;m^{-2}s^{-1}$, 16 hr/day) or complete darkness at $25{\pm}2^{\circ}C$. There was no distinctive difference on callus and embryogenic tissue induction between the two basal media with PGRs. Optimum culture medium appeared to be MS medium supplemented with 1.0mg/L 2,4-D and 0.01mg/L TDZ plus 3% sucrose. Nonembryogenic callus induction rate was not significantly different among the genotypes. However, However, the embryogenic callus induction frequency differed greatly by the genotypes ranging from 55% to 72% when cultured in the dark. Generally, the cultures maintained in the dark tended to show normal somatic embryo development as well as embryogenic tissue formation and this was confirmed by histological examination. Above results suggest that a proper selection of mother tree and dark culture condition are necessary to optimize somatic embryogenesis system of Liriodendron tulipifera.

5본의 백합나무 모수로부터 미숙종자 배양을 통한 체세포 배발생을 시험하였다. 두 가지 배지(MS 및 B5)에 2,4-D 및 TDZ의 농도별 조합처리로 캘러스 및 배발생 조직 유도를 시험하고 체세포배 유도, 발달 및 재분화에 미치는 명 암배양의 효과를 조사하였다. 캘러스 및 배발생 조직의 유도는 두 배지간 유의적인 차이가 없었으나 MS + 2,4-D 1.0 mg/L, TDZ 0.01 mg/L, 3% sucrose 조건에서 양호하게 나타났다. 캘러스 유도는 모수 몇 명 암배양에 따른 차이가 없었으나 배발생 조직의 유도는 암배양이 주효하여 명배양보다 약 2배의 효과가 있었고 모수간 55~72%까지 차이를 나타냈다. 체세포배 유도 및 정상적인 체세포배의 발달에 있어서도 모수의 영향을 받으며, 암배양이 필수적인 것으로 나타났고, 해부학적인 관찰을 통해 확인할 수 있었다. 본 실험 결과는 백합나무 체세포배 유도에 있어 모수의 선택과 암배양이 중요한 요인임을 시사해 준다.

Keywords

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