Response of Ozone Sensitivity in Open-Pollinated Families of Pinus densiflora Seedlings

소나무 풍매차대묘(風媒次代苗)의 가계간(家系間) 오존에 대한 민감성(敏感性) 반응(反應)

  • Lee, Jae-Cheon (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Oh, Chang-Young (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Han, Sim-Hee (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kim, Jang-Su (Department of Forest Genetic Resources, Korea Forest Research Institute)
  • 이재천 (국립산림과학원 산림유전자원부) ;
  • 오창영 (국립산림과학원 산림유전자원부) ;
  • 한심희 (국립산림과학원 산림유전자원부) ;
  • 김장수 (국립산림과학원 산림유전자원부)
  • Received : 2005.03.24
  • Accepted : 2005.05.06
  • Published : 2005.06.30

Abstract

This studies were conducted to compare ozone sensitivity among half-sib families of Japanese red pine (Pinus densiflora S. et Z.). 90 families were exposed in walk-in type chambers to charcoal-filtered air and 100 ppb ozone for 8 hours daily, 5 consecutive months. We surveyed the foliar injury, Relative DRC (diameter at root collar) growth and relative dry weight after fumigation. After 5 months of exposure, foliar injury ranged from 1.9% at tolerant group to 15.2% at sensitive group. Relative DRC growth rates showed increased 12% at tolerant group, but reduced 23% at sensitive group. Total dry weight increased 17% at tolerant group, otherwise reduced by 33% at sensitive group, relative to the control. The root biomass showed more sensitive than the other part biomass. On the basis of the results of these screening studies, families Kangwon 04, 16, 26, 27 and 73 were determined to be tolerant; Kangwon 40, 52, 83, Gyeonggi 01 and Gyeongbuk 20 to be sensitive to ozone. Considerable genetic variation in ozone responses occurred, as shown by differences in sensitivities among families and among groups. These results indicate that tolerant groups of P. densiflora exhibited a high degree of resistance to ozone pollution injury.

본 연구는 우리나라 소나무 90가계를 대상으로 오존에 대한 민감성을 비교하기 위하여 인공광형 생장상에서 100 ppb의 오존을 5개월간 처리하였다. 잎의 가시적 피해, 근원경 생장 및 개체건중량 등을 조사하였다. 잎의 가시적 피해는 내성그룹이 1.9%인 반면 민감성 그룹은 15.20%로 나타났다. 상대근원경 생장비는 내성그룹이 12% 생장한 반면, 민감성 그룹은 23%의 생장 감소를 보였다. 개체 건중량에서도 내성그룹은 17%의 증가를 보였으나 민감성 그룹에서는 33%의 감소를 보였다. 수체 부위별로는 뿌리부분이 가장 민감한 반응을 보였다. 모든 조사 요인을 표준화한 결과 강원 4, 16, 26, 27, 73호가 오존에 대한 내성 가계로 나타났고, 강원 40, 52, 83, 경기 1, 경북 20호 등이 민감한 가계로 나타났다. 이와 같이 가계수준에서 그룹간에 민감성의 차이로 보아 오존에 대한 유전적 변이를 확인 할 수 있었다. 이러한 연구결과로 오존에 대한 가시적 피해가 상대적으로 적고, 근원경 생장과 건중량의 감소율이 낮은 내성그룹이 확인되었다.

Keywords

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