Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Extraction and Determination of Chlorophyll Contents of Korean Pine Needles Using Acetone and DMSO (Dimethylsulfoxide)

아세톤과 DMSO (Dimethylsulfoxide)로 추출(抽出)하여 측정(測定)한 잣나무 침엽(針葉) 내(內) 엽록소(葉綠素) 함량(含量) 비교(比較)

  • Seo, Keum Young (Division of Environmental Science and Ecological Engineering, Korea University) ;
  • Son, Yowhan (Division of Environmental Science and Ecological Engineering, Korea University) ;
  • Koo, Jin Woo (Division of Environmental Science and Ecological Engineering, Korea University) ;
  • Noh, Nam Jin (Division of Environmental Science and Ecological Engineering, Korea University) ;
  • Yi, Myong Jong (College of Forest Sciences, Kangwon National University)
  • 서금영 (고려대학교 환경생태공학부) ;
  • 손요환 (고려대학교 환경생태공학부) ;
  • 구진우 (고려대학교 환경생태공학부) ;
  • 노남진 (고려대학교 환경생태공학부) ;
  • 이명종 (강원대학교 산림자원학부)
  • Received : 2005.05.25
  • Accepted : 2005.06.15
  • Published : 2005.09.30
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Abstract

Chlorophyll contents in Korean pine (Pinus koraiensis) needles of an advance growth stand and a plantation were compared using 80% acetone and DMSO (dimethylsulfoxide). Total chlorophyll contents in DMSO were 4-6 times higher than those in 80% acetone. There were no significant differences in chlorophyll contents between two stand types in both 80% acetone and DMSO. It appeared that 6 hours in DMSO would be appropriate to successfully extract chlorophyll from Korean pine needles of advance growth and planted trees.

참나무류 임분에 자연 발생한 잣나무와 인공 조림된 잣나무 침엽을 80% acetone과 DMSO(Dimethylsulfoxide)로 추출하여 측정한 엽록소 함량을 비교하였다. 80% acetone에 비해 DMSO로 추출한 총 엽록소 함량은 잣나무 침엽의 모든 연령에서 약 4-6배 높은 값을 나타했다. 또한 DMSO로 추출한 경우 상층 임관을 점유한 인공 조림된 잣나무와 하층에 생육하는 잣나무 간에는 총 엽록소 함량에 차이가 없었으며, 80% acetone으로 추출한 경우에도 역시 두 종류의 잣나무 사이에 연령별 총 엽록소 함량의 차이는 없었다. DMSO로 추출할 경우 자연 발생 잣나무와 인공 조림된 잣나무 침엽 모두 6시간의 추출이 적절한 것으로 판단된다.

Keywords

Acknowledgement

Supported by : 농림부

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