Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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A Comparative Study on the Determination of the Selenium in Biological Samples using 75Se and 77mSe Nuclides

75Se과 77mSe 핵종을 이용한 생물시료 중의 셀렌 분석에 대한 비교 연구

  • Moon, Jong-Hwa (HANARO Utilization Technology Development Division, Korea Atomic Energy Research Institute) ;
  • Kang, Sang-Hoon (HANARO Utilization Technology Development Division, Korea Atomic Energy Research Institute) ;
  • Kim, Sun-Ha (HANARO Utilization Technology Development Division, Korea Atomic Energy Research Institute) ;
  • Chung, Yong-Sam (HANARO Utilization Technology Development Division, Korea Atomic Energy Research Institute) ;
  • Kim, Young-Jin (HANARO Utilization Technology Development Division, Korea Atomic Energy Research Institute)
  • 문종화 (한국원자력연구소 하나로이용기술개발부) ;
  • 강상훈 (한국원자력연구소 하나로이용기술개발부) ;
  • 김선하 (한국원자력연구소 하나로이용기술개발부) ;
  • 정용삼 (한국원자력연구소 하나로이용기술개발부) ;
  • 김영진 (한국원자력연구소 하나로이용기술개발부)
  • Received : 2004.09.30
  • Accepted : 2005.01.25
  • Published : 2005.02.25
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Abstract

Se is well known as an anti-oxidant trace element and many customers are interested in an analysis and contents of Se in the various foodstuff samples. This study was aimed at establishig and comparing an analytical method for the determination of the Se in biological samples by neutron activation analysis using two nuclides, $^{75}Se$ and $^{77m}Se$. Keeping this objective, three NIST biological standard reference materials were chosen and the concentrations of the Se were determined under the prefixed analytical conditions such as the irradiation, decay and measurement time. The measured values by both analytical methods were evaluated with certified values. In addition, the detection limits and measurement uncertainty for the analytical results using $^{75}Se$ and $^{77m}Se$ were compared with each other.

셀렌은 항산화 작용을 하는 미량 원소로 잘 알려져 있으며 많은 고객들은 다양한 식품시료중의 셀렌의 농도 및 정량에 관심을 갖고 있다. 본 연구에서는 $^{75}Se$과 $^{77m}Se$ 핵종을 이용한 중성자 방사화 분석법에 의한 생물시료중의 셀렌 분석법을 확립하고 비교하고자 하였다. 이를 위하여 미국표준연구원의 3종의 인증 표준물질을 선택하여 미리 설정된 분석조건 (조사, 냉각 및 측정시간) 하에서 셀렌의 농도를 결정하였다. 두 가지 분석방법에 의한 측정값을 인증값과 평가하였으며 $^{75}Se$과 $^{77m}Se$의 측정값에 대한 검출한계와 불확도를 서로 비교 하였다.

Keywords

References

  1. G. Yang, S. Yin, R. Zhou, L. Gu, B. Yan, Y. Liu, J. Trace Elem. Electrolytes Health, 3(3), 129(1989)
  2. J. G. Yang, K. E. Hill, R. F. Burk, Journal of Nutrition, 119, 1010(1989) https://doi.org/10.1093/jn/119.7.1010
  3. W. C. Willet, M. J. Stamper, British Medical Journal, 297, 573(1988) https://doi.org/10.1136/bmj.297.6654.988
  4. W. C. Hawkes, F. Z. Alkan, L. Oehler, Journal of Nutrition, 133, 3443(2003)
  5. J. R. Arthur, R. C. Mckenzie, G. J. Beckett, Journal of Nutrition, 133, 1457(2003)
  6. WHO, 'Trace Elements in Human Nutrition and Health, Geneva', World Health Organization, Belgium, 1996
  7. C. K. Baskett, V. L. Spate, M. M. Mason, T. A. Nicholas, A. Williams, I. M. Dubman, A. Gudino, J. Denison, J. S. Morris, J. Radioanal. Nucl. Chem., 249(2), 429(2001) https://doi.org/10.1023/A:1013295325692
  8. J. Kucera, P. Bode, V. Stepanek, J. Radioanal. Nucl. Chem., 245(1), 115(2000) https://doi.org/10.1023/A:1006760726572
  9. Y. S. Chung, J. H. Moon, S. H. Kim, S. Y. Baek,' Applied Research and Development of Neutron Activation Analysis', KAERI/RR-2031/99, KAERI, Korea, 1997
  10. L. A. Currie, Anal. Chem., 40, 587(1968) https://doi.org/10.1021/ac60259a007