Optimization of Cholesterol Removal by Crosslinked ${\beta}$-Cyclodextrin in Egg Yolk

  • Jung, Tae-Hee (Department of Food Science and Technology, Sejong University) ;
  • Park, Heung-Sik (Department of Food Science and Technology, Sejong University) ;
  • Kwak, Hae-Soo (Department of Food Science and Technology, Sejong University)
  • Published : 2005.12.31


Optimum conditions for cholesterol removal in egg yolk were evaluated based on ratio of egg yolk-to-water, crosslinked ${\beta}$-cyclodextrin (${\beta}$-CD) concentration, and mixing temperature, time, and speed by adding crosslinked ${\beta}$-CD treated with adipic acid. Cholesterol removal in egg yolk-water mixture increased with increasing ${\beta}$-CD level (10-25%). About 95% was removed by 25% ${\beta}$-CD at 1:1 ratio of egg yolk-to-water and 800 rpm mixing at $40^{\circ}C$ for 30 min. In recycling study, removal rates were measured using ten times recycled crosslinked ${\beta}$-CD in egg yolk, and 85% cholesterol removal was observed with eight times reuse. These results indicated that over 90% cholesterol was removed at 1:1 ratio of egg yolk-to-water, 20% crosslinked ${\beta}$-CD addition, and 30 min mixing with 600 rpm at $40^{\circ}C$.


  1. Ann. NY Acad. Sci. v.598 Dietary recommendation to prevent coronary heart disease Brown, W.V.
  2. Food Technol. no.Oct. Consumer evaluation of reducedcholesterol butter Schroder, B.G.;Baer, R.J.
  3. J. Dairy Sci. v.72 Removal of cholesterol from milk fat using supercritical carbon dioxide Bradley, R.L. Jr.
  4. Wall Street Journal Fear of food
  5. World Poultry Sci. J. v.48 Manipulation of egg yolk cholesterol: a physiologist's view Griffin, H.D.
  6. J. Food Sci. v.55 Extraction of cholesterol and other lipids from dried egg yolk using supercritical carbon dioxide Froning, G.W.;Wehling, R.L.;Cuppett, S.L.
  7. Designs and analysis of experiments in the animal and medical sciences Griffin, H.D.
  8. World Poultry Sci. J. v.44 ModifYing egg yolk cholesterol - A review Hargis, P.S.
  9. Low cholesterol dried egg yolk and process Melnick, D.
  10. Functional decholesterolized egg yolks Merchant, Z.M.;Anikumar, G.G.;Krishnamurthy, R.G.
  11. Low cholesterol egg product and process Frioriti, J.A.;Rex, J.S.;Clifford, H.
  12. Supercritical fluid extraction of cholesterol from dried egg;Fat and cholesterol reduced foods Froning, G.W.;Haberstroh, C.(ed.);Morris, C.E.(ed.)
  13. J. Food Sci. v.60 Cholesterol reduction in liquid egg yolk using ${\beta}$-cyclodextrin Smith, D.M.;Awad, A.C.;Bennink, M.R.;Gill, J.L.
  14. Cholesterol reduction. Commonwealth scientific and industrial research organization Oakenfull, D.G.;Sihdu, G.S.
  15. Process for the removal of ,${\beta}$-cyclodextrin from egg yolk Cully, J.;Vollbrecht, H.R.
  16. Method for reduction of cholesterol in egg materials Awad, A.Z.;Smith, D.M.
  17. Cholesterol removal from animal with cyclodextrin in inclusion Makoto, K.;Akio, O.;Reijiro, S.
  18. J. Dairy Sci. v.82 Cholesterol removal from homogenized milk with ${\beta}$-cyclodextrin Lee, D.K.;Ahn, J.;Kwak, H.S.
  19. J. Agric. Food Chem. v.50 Removal of cholesterol from Cheddar cheese by ${\beta}$-cyclodextrin Kwak, H.S.;Jung, C.S.;Shim, S.Y.;Ahn, J.
  20. J. Dairy Sci. v.86 Functional properties of cholesterolremoved whipping cream treated by ${\beta}$-cyclodextrin Shim, S.Y.;Ahn, J.;Kwak, H.S.
  21. Asian Austral. J. Anim. The effect of evening primrose oil on chemical properties and lowering blood cholesterol in nutraceutical Cheddar cheese Kim, J.J.;Yoo, S.H.;Ahn, J.;Jeon, W.M.;Kwak, H.S.
  22. Asian Austral. J. Anim. v.18 Development of phytosterol esteradded Cheddar cheese for lowering blood cholesterol Kwak, H.S.;Ahn, H.J.;Ahn, J.
  23. Int. J. Dairy Technol. Property changes and cholesterol lowering effect in evening primrose oil-added and cholesterol-reduced yogurt Lee, S.J.;Hwang, J.S.;Lee, S.;Ahn, J.;Kwak, H.S.
  24. Asian Austral. J. Anim. v.7 Change of properties and cholesterol lowering effect in evening primrose oil-added and cholesterol-reduced milk Hwang, J.H.;Lee, S.J.;Kwak, H.S.
  25. Asian Austral. J. Anim. v.18 Cholesterol removal from homogenized milk with crosslinked ${\beta}$-cyclodextrin by adipic acid Han, E.M.;Kim, S.H.;Ahn, J.;Kwak, H.S.
  26. Chem. Eng. News v.30 Industrial starch research Cardwell, C.G.
  27. J. Assoc. Anal. Chem. v.69 Evaluation of direct saponification method in determination of cholesterol in meats Adams, M.L.;Sullivan, D.M.;Smith, R.L.;Richer, E.F.
  28. Asian Austral. J. Anim. v.14 Optimization of ${\beta}$-cyclodextrin recycling process for cholesterol removal in cream Kwak, H.S.;Suh, H.M.;Ahn, J.;Kwon, H.J.
  29. Users Guide: Statistics SAS${\circledR}$
  30. Process for eliminating cholesterol contained in a fatty substance of animal origin and the fatty substance with reduced cholesterol obtained Courregelongue, J.;Maffiand, J.P.
  31. J. Food Sci. v.60 Cholesterol removal from lard-mixture with ${\beta}$-cyclodextrin Yen, G.C.;Tsui, L.T.
  32. Food Res. Int. v.37 Immobilization of ${\beta}$-cyclodextrin in chitosan beads for separation of cholesterol from egg yolk Chiu, S.H.;Chung, T.W.;Giridhar, R.;Wu, W.T.
  33. Arch. Pharm. Res. v.27 Immobilized ${\beta}$-cyclodextrin as a simple and recyclable method for cholesterol removal in milk Kwak, H.S.;Kim, S.H.;Kim, J.H.;Choi, H.S.;Kang, J.M.
  34. Arch. Pharm. Res. v.27 Crosslinking of ${\beta}$-cyclodexrin on cholesterol removal from milk Kim, S.H.;Ahn, J.;Kwak, H.S.
  35. J. Agric. Food Chem. v.38 Behaviors of polymer supported digitonin with cholesterol in the absence and presence of butter oil Micich, T.J.
  36. Korean J. Dairy Sci. v.20 Conditions of the removal of cholesterol from milk by treatment with saponin Oh, H.I.;Jang, E.J.;Kwak, H.S.