The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Antioxidant Property of the Gagam-Hyungbang-Gihwang-tang Using Biochemical Markers of Carcinogenesis

가감형방지황탕 열수 추출물이 항산화 작용에 미치는 영향

  • Han Jin-Soo (Department of Oriental Ophthalmology & Otolaryngology & Dermatology, College of Oriental Medicine, Wonkwang University) ;
  • Park Seong-Sik (Department of Oriental Ophthalmology & Otolaryngology & Dermatology, College of Oriental Medicine, Wonkwang University)
  • 한진수 (동국대학교 한의과대학 사상체질과) ;
  • 박성식 (동국대학교 한의과대학 사상체질과)
  • Published : 2005.09.01
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Abstract

Objectives : The verity extract of the Gagam-Hyungbang-Gihwang-tang (GHG) was assessed to determine the mechanisms of its antioxidant activity. Methods : The fellowing effects were measured : GHG exhibited a concentration-treatment; scavenging ${\alpha},\;{\alpha}-diphenyl-\beta-picrylhydrazyl$ (DPPH) radical, linoleic acid oxidation in a thiocyanate assay system, and superoxide anion, hydroxyl radical-induced DNA nicking. We investigated mRNA levels such as superoxide-dismutase. Results : The GHG extract showed dose-dependent free radical scavenging activity, including DPPH radicals, hydroxyl radicals, and superoxide anion, using different systems. The GHG was also found to be effective in protecting plasmid DNA against the strand breakage induced by hydroxyl radicals in Fenton's reaction mixture. Furthermore, SOD-1 mRNA expression levels increased in tat hepatoma H4IIE cells Conclusions : We expect that GHG will to helpful to the development of antioxidant activity treatments.

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