대한한의학회지 (The Journal of Korean Medicine)
- 제26권3호
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- Pages.135-145
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- 2005
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- 1010-0695(pISSN)
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- 2288-3339(eISSN)
Sodium Cyanide로 유도된 신경아세포종 세포주에서 오수유의 신경상해 보호효과
Anti-neuronal Injury Effect of Evodiae Fructus Water Extract in Sodium Cyanide-induced SK-N-SH Cell Lines
-
장우석
(대구한의대학교 한의학과 내과학전공)
;
- 이소연 (대구한의대학교 한의학과 내과학전공) ;
- 윤현덕 (대구한의대학교 한의학과 내과학전공) ;
- 신오철 (대구한의대학교 한의학과 내과학전공) ;
- 박창국 (대구한의대학교 한의학과 내과학전공) ;
- 박치상 (대구한의대학교 한의학과 내과학전공)
-
Jang Woo-Seok
(Department of Oriental Medicine Graduate School of Daegu Haany University)
;
- Lee So-Yeon (Department of Oriental Medicine Graduate School of Daegu Haany University) ;
- Yoon Hyeon-Deok (Department of Oriental Medicine Graduate School of Daegu Haany University) ;
- Shin Oh-chul (Department of Oriental Medicine Graduate School of Daegu Haany University) ;
- Park Chang-Gook (Department of Oriental Medicine Graduate School of Daegu Haany University) ;
- Park Chi-Sang (Department of Oriental Medicine Graduate School of Daegu Haany University)
- 발행 : 2005.09.01
초록
Objectives : This study investigated effect of Evodiae fructus water extract (EVOR) on apoptotic cell death induced by NaCN in SK-N-SH neuroblastoma cell lines. NaCN stimulates glutamate release which can activate glutamate receptors to initiate excitotoxic processes. This study examines the role of EVOR in mediating NaCN-induced cytotoxicity. Methods & Results : Cytotoxicity was assessed by measuring lactate dehydrogenase (LDH) in the culture media. NaCN(0.1mM) produced cytotoxicity following 12hrs of incubation. NaCN-induced cytotoxicity was partially blocked by EVOR. The treatment of EVOR in simultaneous exposure of cultures to NaCN provided complete protection against cytotoxicity. NaCN-induced cytotoxicity was found to inhibit DNA fragmentation, repaired by cell cycle and simultaneous exposure to NaCN, regenerated with neurite outgrowh by EVOR. These results indicate thaf damage by NaCN in neumnal cell cultures was repaired by EVOR, whereas NaCN-induced cytotoxicity is blocked Primarily by activation of anti-apoptosis. Conclusions : These results suggest that EVOR may be beneficial for the treatment of dementia and other degenerative problems of the central nervous system.