한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

한국작물학회 (The Korean Society of Crop Science)
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자광찰벼 추출물의 H4IIE 세포에서의 항산화 효과

Antioxidant Activity of Jakwangchalbyeo Extracts in H4IIE Cells

  • 발행 : 2005.11.01
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초록

자광찰벼, 화선찰벼, 및 일품벼 에탄올 분획의 H4IIE 세포에 대찬 산화적 스트레스 보호효과를 검정하기 위해서 수행하였다. 과산화수소에 의하여 생성된 자유라디칼은 H4IIE 세포에 산화적 손상을 초래하며 이러한 손상에 기인된 세포사멸에 대하여 화선찰벼와 일품벼 추출물은 방어효과가 없었으나, 적색을 띤 자광찰벼 추출물의 에탄올 분획은 과산화수소에 의하여 유도된 산화적 스트레스에 가장 현저한 효과를 나타내었다. 금후, 보다 광범위하고 집중적인 연구를 수행한다면 쌀 함유 유효성분들의 생리활성에 관한 보다 명확한 효과가 구명될 수 있을 것으로 사료된다.

Experiments were performed to investigate the effects of ethanol fraction of three different rice (Oryzasativa L. var. japonica) grain (Jakwangchalbyeo, red-pericarp glutinous rice; Hwasunchalbyeo, white-pericarp glutinous rice; Ilpumbyeo, white-pericarp non-glutinous rice) extracts on the protection of oxidative stress. Antioxidant activities of ethanol fraction of rice grain extracts were made with MTT cell viability assay in H4IIE cell that is challenged with hydrogen peroxide. Hwasunchalbyeo extract and Ilpumbyeo extract did not show any significant protective effects on the $H_2O_2-induced$ oxidative stress in B4IIE cells, and Jakwangchalbyeo extract improved the cell viability up to $82\%\;and\;74\%$ at concentration of $100{\mu}g/mL$ for 5 h and 24 h treatment, respectively. In conclusion, red-pericarp Jakwangchalbyeo extract as compared with other rice extracts exerted significant inhibitory effects on the hydyogen peroxideinduced oxidative stress in the H4IIE cells.

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