Applied Biological Chemistry

  • 제48권4호
  • /
  • Pages.421-424
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  • 2005
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  • 2468-0834(pISSN)
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  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

식용식물자원으로부터 활성물질의 탐색-XV. 머위(Petasites japonicus)잎으로부터 Triterpene 배당체의 분리

Development of Biologically Active Compound from Edible Plant Sources-XV. Isolation of Triterpene Glycosides from the Leaf of Petasites japonicus

  • 방면호 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 박진규 ((주)파마코디자인) ;
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 양혜정 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 유종수 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 안은미 ((주)싸이제닉) ;
  • 김대근 (우석대학교 약학대학) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Bang, Myun-Ho (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Park, Jin-Kyu (Pharmaco Design Co. Ltd.) ;
  • Song, Myoung-Chong (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Yang, Hye-Joung (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Yoo, Jong-Su (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Ahn, Eun-Mi (Scigenic Co. Ltd.) ;
  • Kim, Dae-Keun (Department of Pharmacy, Woosuk University) ;
  • Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center)
  • 발행 : 2005.12.31
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초록

머위 잎을 80% MeOH수용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 $H_2O$로 용매 분획하였다. EtOAc 분획에 대하여 silica gel과 ODS column chromatography를 반복하여 2종의 triterpenoid 화합물을 분리하였다. 각 화합물은 $^1H-{^1H}$ gCOSY, gHMBC 및 gHSQC와 같은 2D-NMR기법을 포함한 스펙트럼 데이터를 해석하여 rosamutin(1) 및 peduncloside(2)로 구조를 결정하였다.

The leaf of Petasites japonicus was extracted with 80% aqueous MeOH and solvent fractionated with EtOAc, n-BuOH and water successively. From the EtOAc fractions, two triterpenoids were isolated through the repeated silica gel and ODS column chromatographies. The chemical structures of the isolated terpenoids were determined as rosamutin (1) and peduncloside (2) through the interpretation of several spectral data including 2D-NMR such as $^1H-{^1H}$ gCOSY, gHSQC and gHMBC.

키워드

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