Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Molecular Cloning of a cDNA Encoding Ribulose-1,5-bisphosphate Carboxylase Small Subunit (rbcS) from Panax ginseng C. A. Meyer

고려인삼의 Ribulose-1,5-Bisphosphate Carboxylase Small Subunit(rbcS) 유전자의 분리 및 특성분석

  • In Jun Gyo (Research Institute of Biotechnology, BioPia Co., Ltd.) ;
  • Lee Bum Soo (Research Institute of Biotechnology, BioPia Co., Ltd.) ;
  • Youn Jae-Ho (Dept. of Oriental Medicine Material and Processing, Kyung Hee University) ;
  • Son Hwa (Dept. of Oriental Medicine Material and Processing, Kyung Hee University) ;
  • Yi Tae Hoo (Dept. of Oriental Medicine Material and Processing, Kyung Hee University) ;
  • Yang Deok Chun (Dept. of Oriental Medicine Material and Processing, Kyung Hee University)
  • 인준교 ((주)바이오피아 생명공학연구소) ;
  • 이범수 ((주)바이오피아 생명공학연구소) ;
  • 윤재호 (경희대학교 한방재료가공학과) ;
  • 손화 (경희대학교 한방재료가공학과) ;
  • 이태후 (경희대학교 한방재료가공학과) ;
  • 양덕춘 (경희대학교 한방재료가공학과)
  • Published : 2005.10.01
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Abstract

A full-length cDNA encoding ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) has been isolated and its nucleotide sequence determined from root in ginseng plant (Panax ginseng). The rbcS cDNA of ginseng is 790 nucleotides long and has an open reading frame of 549 bp with deduced amino acid of 183 residues (pI 8.37), 20.5 kDa. The deduced amino acid sequence of rbcS matched to the previously reported rbcS protein genes and showed a high similarity with the $78\%$ identity with rbcS of Helianthus annuus (CAA68490). In the phylogenetic analysis based on the amino acid residues, the ginseng rbcS was clustered with H. annuus (CAA68490), C. morifolium (AA025119) and L. sativa (Q40250).

고려 인삼(Panax ginseng)의 뿌리로부터 ribulose-1,5-bisphosphate carboxylase small subunit(rbcS) 유전자를 선발하여 sequence 분석을 실시하였다. 고려 인삼 rbcS cDNA는 790 bp 염기로 구성되어 있으며, 183개의 아미노산(pI 8.37)을 코드하는 549 bp의 ORF를 가지고 있고 단백질의 분자량은 20.5 kDa으로 추정되었다. 인삼 rbcS는 기존에 보고된 것과 유사성을 나타내었으며, Helianthus annuus(CAA68490)에서 분리된 것과 $78\%$의 높은 상동성을 보였다. 기존에 데이터베이스에 축적되어 있는 다른 식물체로부터 분리된 rbcS와 아미노산 서열을 비교한 결과 인삼의 ybcS는 H. annuus (CAA68490), C. morifolium (AAO25119), L. sativa (Q40250)와 밀접한 유연관계에 있는 것으로 조사되었다.

Keywords

References

  1. Akazawa T., T. Takabe and H. Kobayashi. 1984. Molecular evolution of ribulose-l ,5-bisphosphate carboxylase/oxygenase. Trends Biochem. Sci. 9:380-383 https://doi.org/10.1016/0968-0004(84)90219-6
  2. Altschul S.F., W. Gish , W. Miller, E.W. Myers and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2
  3. Dean C., E. Pichersky and P. Dunsmuir. 1989. Structure, evolution and regulation of rbcS genes in higher plants. Ann. Rev. Plant Physiol. Plant Mol. Biol. 40:415-439 https://doi.org/10.1146/annurev.pp.40.060189.002215
  4. Ellis R.J. 1981. Chloroplast proteins: synthesis, transport and assembly. Annu. Rev. Plant Physiol. 32:111-137 https://doi.org/10.1146/annurev.pp.32.060181.000551
  5. Gilmartin, P.M., L. Sarokin, J. Memelink and N.H. Chua. 1990. Molecular light switches for plant genes. The Plant Cell 2:369-378 https://doi.org/10.2307/3869087
  6. Jensen R.G. and J.T Bahr. 1977. Ribulose 1,5bisphosphate carboxy lase oxygenase. Annu. Rev. Plant Physiol. 28:379-400 https://doi.org/10.1146/annurev.pp.28.060177.002115
  7. Keegstra K., L.J. Olsen and S.M. Theg. 1989. Chloroplastic precursors and their transport across the envelope membranes. Annu. Rev. Plant Physiol. Plant Mol. Biol. 40:471-501 https://doi.org/10.1146/annurev.pp.40.060189.002351
  8. Kuhlmeier C., P.J. Green and Chua M.H. 1987. Regulation of gene expression in higher plant. Ann. Rev. Plant Physiology 38:221-257 https://doi.org/10.1146/annurev.arplant.38.1.221
  9. Lee, J.H., Y.P. Lim, J.C. Park and K.T. Choi. 1995. Cloning of ribulose-1 ,5-bisphosphate carboxylase/ oxygenase large subunit (rbcL) gene from Korean ginseng (Panax ginseng C.A. Meyer). Korean J. Ginseng Sci. 19:51-55
  10. Manzara, T. and W. Gruissem. 1988. Organization and expression of the genes encoding ribulose-I,5bisphosphate carboxy lase in higher plants. Photosynth. Res. 16:117-139 https://doi.org/10.1007/BF00039489
  11. Morris P.C., A. Kumar, D.J. Bowles and A.C. Cuming. 1990. Osmotic stress and abscisic acid regulate the expression of the Em gene of wheat. Eur. J. Biochem. 190:625-630 https://doi.org/10.1111/j.1432-1033.1990.tb15618.x
  12. Stiekema W.S., C.F. Wimpee, J. Silverthorne and E.MTobin. 1983. Phytochrome control of the expression of two nuclear genes encoding chloroplast proteins in Lemna gibba L. Plant Physiology 72:717-724 https://doi.org/10.1104/pp.72.3.717
  13. Thompson J.D., D.G. Higgins and T.J. Gilbson. 1994 CLUST AL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-secific gap penalties and weight matrixchoice. Nucleic Acids Res. 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673
  14. Tobin E.M. 1981. Phytochrome mediated regulation of messenger RNAs for the small subunit of ribulose1,5-bisphosphate carboxylase and light harvesting chrolophyll alb protein in Lemna gibba. Plant Mol. Biol. 1 :35-51 https://doi.org/10.1007/BF00023012
  15. Tobin E.M. and J. Silverthorne. 1985. Light regulation of gene expression in higher plants. Ann. Rev. Plant Physiol. 36:569-593 https://doi.org/10.1146/annurev.pp.36.060185.003033