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Effects of Hydrolysis Time on the Molecular Weight Distribution of Alginates Prepared from Sea Tangle Laminaria japonicus

  • Lim Yeong-Seon (Department of Food Science, Kangnung National University) ;
  • You Byeong-Jin (Department of Food Science, Kangnung National University)
  • Published : 2005.09.01

Abstract

To prepare oligouronic acids from high-molecular-weight alginates, sea tangle Laminaria japonicus alginates were hydrolyzed at $80^{\circ}C$ for various hydrolysis times (HT). The effects of hydrolysis time on the distribution ratios (DR) of the molecular weights (MW) of the hydrolyzed alginates were in-vestigated. As HT increased, the DR of the alginates with MW>500 kDa decreased ex-ponentially, while those with MW=50-100 kDa or MW<50 kDa increased exponentially. For MW=300-500 kDa, DR increased exponentially until HT reached 60 min, and then decreased exponentially. Similarly, for MW=100-300 kDa, DR increased exponentially until HT reached 90 min, and then decreased.

Keywords

References

  1. AOAC. 1995. Official Methods of Analysis, 16th ed., Chapter 44, p. 3. Association of Official Analytical Chemists. Arlington, VA, USA
  2. Chen, S.L., A.I. Yeh and S.B. Wu. 1997. Effects of particle radius, fluid viscosity and relative velocity on the surface heat transfer coefficient of spherical particles at low Reynolds numbers. J. Food Eng., 31, 473-484 https://doi.org/10.1016/S0260-8774(96)00093-3
  3. Fujihara, M. and T. Nagumo. 1993. An influence of the structure of alginate on the chemotactic activity of macrophages and the antitumor activity. Carbohydr. Res., 243, 211-215 https://doi.org/10.1016/0008-6215(93)84094-M
  4. Fujiki, K., H. Matsuyama and T. Yano. 1994. Protective effect of sodium alginates against bacterial infection in common carp, Cyprinus carpio. L. J. Fish Dis., 17, 349-354 https://doi.org/10.1111/j.1365-2761.1994.tb00230.x
  5. Hajime, O., S. Yasushi, Y. Kanto, U. Isamu and K. Koichi. 1994. Possible antitumor promoting properties of marine algae and in vitro activity of Wakame seaweed extract. Biosci. Biotech. Biochem., 56, 994-999 https://doi.org/10.1271/bbb.56.994
  6. Haug, A. and B. Larsen. 1962. Quantitative determination of uronic acid composition of alginates. Acta Chem. Scand., 16, 1908-1918 https://doi.org/10.3891/acta.chem.scand.16-1908
  7. Haug, A., B. Larsen and O. Smidsrod, 1966. A study of the constitution of alginic acid by partial acid hydrolysis. Acta Chem. Scand., 20, 183-190 https://doi.org/10.3891/acta.chem.scand.20-0183
  8. Haug, A., B. Larsen and O. Smidsrod, 1967. Studies on the sequence of the uronic acid residues in alginic acid. Acta Chem. Scand., 21, 691-704 https://doi.org/10.3891/acta.chem.scand.21-0691
  9. Hidaka, H., T. Eida, T. Takizawa, T. Tokuzawa and Y. Tashiro. 1986. Effect of fructooligosaccharide on intestinal flora and human health. Bifido. Micro., 5, 37-50
  10. Hideki, O., S. Jitsuo and K. Yoshinari. 1993. Direct control of the constituents ratio in a wide range in alginate produced by Azobacter vinelandii. Biosci. Biotech. Biochem., 57, 332-336 https://doi.org/10.1271/bbb.57.332
  11. Hein, N.Q., N. Nagasawa, L.X. Tham, F. Yoshii, V.H. Dang, H. Mitomo, K. Makuuchi and T. Kume. 2000. Growth-promotion of plants with depolymerized alginates by irradiation. Rad. Phys. Chem., 59, 97-101 https://doi.org/10.1016/S0969-806X(99)00522-8
  12. Kobayashi, N., Y. Kanazawa, S. Yamabe, K. Iwata, M. Nishizawa, T. Yamagishi, O. Nishikaze and K. Tsuji. 1997. Effects of depolymerized sodium alginate on serum total cholesterol in healthy women with a high cholesterol intake. J. Home Econ. Japan, 48, 255-230
  13. Mancini, M., M. Moresi and F. Sappino. 1996. Rheological behaviour of aqueous dispersions of algal sodium alginates. J. Food Engr., 28, 283-295 https://doi.org/10.1016/0260-8774(95)00068-2
  14. Nagasawa, N., H. Mitomo, F. Yoshii and T. Kume. 2000. Radiation-induced degradation of sodium alginate. Polymer Degrad. Stabil., 69, 279-285 https://doi.org/10.1016/S0141-3910(00)00070-7
  15. Penman, A. and G.R. Sanderson. 1972. A method for the determination of uronic acid sequence in alginate. Carbohydr. Res., 25, 273-282 https://doi.org/10.1016/S0008-6215(00)81637-7
  16. Sime, W.J. 1990. Alginates. In: Food Gels, Harris, P., ed. Elsevier, London, UK, pp. 53-78
  17. Suzuki, T., K. Nakai, Y. Yoshie, T. Shirai and T. Hirano. 1993. Digestibility of dietary fiber in brown alga, kombu, by rats. Nippon Suisan Gakkaishi, 59, 879-884 https://doi.org/10.2331/suisan.59.879
  18. Turquois, T. and H. Gloria. 2000. Determination of the absolute molecular weight average and molecular weight distributions of alginates used as ice cream stabilizers by using multiangle laser light scattering measurements. J. Agric. Food Chem., 48, 5455-5458 https://doi.org/10.1021/jf000003v
  19. You, B.J., Y.S. Im, I.H. Jeong and K.H. Lee. 1997. Effect of extraction conditions on bile acids binding capacity in vitro of alginate extracted from sea tangle (Laminaria spp.). J. Kor. Fish. Soc., 30, 31-38
  20. You, B.J. and Y.S. Lim. 2003. Effects of extracting and drying method on physical properties of alginates from sea tangle, Laminaria japonica. J. Kor. Fish. Soc., 36, 340-345
  21. You, B.J., Y.S. Lim and H.S. Ryu. 2004. Effects of hot water treatment and dialysis on measuring the average molecular weight of alginates. J. Kor. Fish. Soc., 37, 1-6

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