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목초액 및 코코넛액이 석곡(Dendrobium moniliforme)의 유묘 증식과 기내 개화에 미치는 영향

The Effect of Pyroligneous Liquor and Coconut Water on Plantlet Multiplication and in Vitro Flowering of Dendrobium moniliforme

  • Jee, Sun-Ok (Department of Biotechnology, Joongbu University) ;
  • Cho, Dong-Hoon (Department of Horticulture, Kyungpook National University)
  • 발행 : 2005.10.01

초록

본 연구는 배지에 첨가된 목초액 및 코코넛액이 석곡 유묘의 증식 및 기내 개화에 미치는 영향을 조사하기 위하여 실시되었다. 목초액이 석곡 유묘의 증식에 미치는 영향은 NAA 0.1 mg/L와 kinetin 1.0 mg/L 가 첨가된 $H_{3} P_{4}$ 배지에 목초액 1.0 ml/L를 처리했을 때 유묘의 증식 및 생장이 양호 하였다. 코코넛액은 30 ml/L가 첨가되었을 때 유묘의 증식 및 생장이 가장 양호하였다. 기내 개화 역시 NAA 0.1 mg/L 와 kinetin 1.0 mg/L 가 첨가된 $H_{3} P_{4}$ 배지에 목초액 1.0 ml/L 또는 코코넛액 30 ml/L가 첨가되었을 때 전체 개체의 $ 20\% $에서 기내개화가 일어났다

This experiment was carried out to clarify the effect of pyroligneous liquor and coconut water on plantlet multiplication and in vitro flowering of Dendrobium moniliforme. Plantlet growth and multiplication was good in 1.0 ml/L pyroligneous liquor treatment which was added to the basal media of 3 g/L hyponex and 4 g/L peptone ($H_{3} P_{4}$) containing 0.1 mg/L NAA and 1.0 mg/L kinetin. In the treatment of 30 ml/L coconut water showed good results on plantlet growth and multiplication. In vitro flowering showed highest rate in the treatment of 1.0 ml/L pyroligneous liquor and 30 ml/L coconut water which were added to the basal media of $H_{3} P_{4}$ containing 0.1 mg/L NAA and 1.0 mg/L kinetin.

키워드

참고문헌

  1. Britto, S. John, E. Natarajan and D. I. Arockiasamy. 2003. In vitro flowering and shoot multiplication from nodal explants of Cerapegia bulbasa Roxb. var. bulbosa. Taiwania 48, 106-111
  2. Carson, Jace A., and David W. M. Leung. 1994. In vitro flowering and propagation of Leptinella nana L., an endangered plant. New zealand J. Bat. 32, 79-83
  3. Chung, J.D., C.K. Chun, YK Suh, and S.Y. Byun. 1981. Studies on aseptic culture of seeds in Oendrabium manile. J. Kor. Soc. Hart. Sci. 22, 139-145
  4. Chung, J.D., J.K. Chun and S.O. Choi. 1985. Asymbiotic Germination of Cymbidium ensifolium (2) Effects of several supplements to the medium , pH values and light and / or dark culture periods on growth of rhizome and organogenesis from rhizome. J. Kor. Soc. Hart. Sci. 26, 186-192
  5. Guillen, M.D. and M.L. Ibargoitia. 1998. New components with potential antioxidant and organoleptic properties, detected for the first time in liquid smoke flavoring preparations. J. Agric Food Chem. 46, 1276-1285 https://doi.org/10.1021/jf970952x
  6. Ichihashi, S. and K Shunji. 1973. Studies on clonal propagation of CattIeya through tissue culture method (1) Factors affecting survival and growth of shoot meristem of Cattleya in vitro. J. Japan Soc. Hort. Sci. 42, 264-270 https://doi.org/10.2503/jjshs.42.264
  7. Jang, C.S. and HD. Seok. 2001. Policy directions for the general use of charcoal and wood tar in agriculture and livestock. J. Res. Forest and economy 9, 28-37
  8. Jee, S.O. 1999. Effect of culture substrate and wood vinegar solution on bulblet increment of Lilium longiflorum. J. Joongbu Univ. 12, 375-382
  9. Jee, S.O. 2002. Effect of pyroligneous liquor on rhizome growth and shoot formation of Cymbidium ensifolium. J. Natural Sci. Joongbu Univ. 11, 81-85
  10. Kim, Y.H., S.K. Kim, KS. Kim and Y.H. Lee. 2001. Compounds identification of commercial wood vinegar liquors. J. Kor. Soc. Agric. Chem. Biotechnol. 44, 262-268
  11. Kotomori, S. and T. Murashige. 1965. Some aspects of aspects of aseptic propagation of orchids. Amer. Orch. Soc. Bull. 34, 484-489
  12. Lee, E.J., and J.S. Lee. 1993. Studies on in vitro cutting of Oendrobium moniliforme native to Korea. Abstract of J. Kor. Soc. Hort. Sci. 11, 314-315
  13. Lee, H.S., KW. Lee and J.R. Liu. 1994. Measurement of determination time of in - vitro flowering in Ginseng (Panax ginseng). Kor. J. Plant Tissue Culture 21, 347-351
  14. Lee, K 1980. The wild orchids of Korea. Jungsung publisher. Seoul. Korea. p. 193
  15. Mun, S.P. and C.S. Ku. 2002. Analysis of volatile compounds in bamboo and wood crude vinegars by the Solid-Phase Microextraction (SPME) method. Mokchae Konghak 30, 80-86
  16. Robert, M.S. Jr. 1966. Stem propagation of Phalaenopsis. Amer. arch. Soc. Bull. 35, 40-42
  17. Stephen, R. and N. Jayabalan. 1998. In vitro flowering and seed setting formation of Coriandum sativum L. Curro Sci. 74, 195-197
  18. Sugiura, G. 1972. In Mokuzai Kokyo Handbook. Forestry and Forest Res. lnst. Maruzen, Tokyo. p. 930
  19. Thiruvengadam, M. and N. Jayabalan. 2001. In vitro flowering of Vitex negundo J. - a medicinal plant. Plant Cell Biotech and Mol. BioI. 2, 67-70
  20. Toth, J. and K. Potthast. 1984. Chemical aspects of the smoking of meat and meat products. Adv. Food Res. 29, 87-158 https://doi.org/10.1016/S0065-2628(08)60056-7
  21. Virupakshi, S., B. R. Manjunatha, and G. R. Naik. 2002. In vitro flower induction in callus from a juvenile explant of sugarcane, Saccharum officinarum L., Var. CoC 671 Current Science 83, 1195-1197
  22. Yatagi, M., G. Unrinin and G. Sugiura. 1986. By-products of wood carbonization. Tar from mangrove, sugi ogalite, wheat straw and chishima-sasa. Mokuzai Gakkaishi 32, 467-471
  23. Yoo, Y.K. and S.W. Kang. 2003. Effects of wood charcoal and pyroligneous acid on the growth and formation of bulblet in lily. Kor. L. Hart. Sci. Technol. 21, 80-81
  24. Zhang, Zemin, and David W.M. Leung. 2000. A comparison of in vitro with in vivo flowering in Gentian. Plant Cell, Tissue and Organ Culture. 63, 223-226 https://doi.org/10.1023/A:1010701332296