Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biodegradation of Phenanthrene by Psychrotrophic Bacteria from Lake Baikal

  • AHN TAE-SEOK (Department of Environmental Science, Kangwon National University) ;
  • LEE GEON-HYOUNG (Department of Biology, Kunsan National University) ;
  • SONG HONG-GYU (Division of Biological Sciences, Kangwon National University)
  • Published : 2005.10.01
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Abstract

Psychrotrophic phenanthrene-degrading bacteria were identified in the sediment samples collected from Lake Baikal, Russia. Among 70 phenanthrene-degrading isolates, the seven that had the highest phenanthrene-degradation rates were identified by 16S rDNA sequencing. Isolate P25, identified as the Gram-positive rod-shaped organism Rhodococcus erythropolis, had the highest growth and degradation rate at $15^{\circ}C$. It could remove $26.0\%$ of 100 mg $1^{-1}$ phenanthrene in 20 days at $15^{\circ}C$, and degradation was less at $5^{\circ}C\;and\;25^{\circ}C$. The addition of surfactants to enhance degradation was tested. Brij 30 and Triton X-100 inhibited degradation at all surfactant concentrations tested, but Tween 80 stimulated phenanthrene degradation, especially at low concentrations. When $20{\times}$ CMC (critical micelle concentration) of Tween 80 was added, $38.0\%$ of 100 mg $1^{-1}$ phenanthrene was degraded in 12 days at $15^{\circ}C$. This psychrotrophic phenanthrene-degrading bacterium is a candidate for use in bioremediation of polycyclic hydrocarbon contamination in low temperature environments.

Keywords

References

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