Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Investigation of Possible Horizontal Gene Transfer from the Leaf Tissue of Transgenic Potato to Soil Bacteria

  • KIM YOUNG TAE (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • KIM SUNG EUN (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • PARK KI DUK (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • KANG TAE HOON (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • LEE YUN MI (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • LEE SANG HAN (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • MOON JAE SUN (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • KIM SUNG UK (Laboratory of Cellular Function Modulator, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2005.10.01
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Abstract

To monitor the possibility of horizontal gene transfer between transgenic potato and bacteria in the environment, the gene flow from glufosinate-tolerant potato to bacteria in soils was investigated. The soil samples treated with the leaf tissue of either glufosinate-tolerant or glufosinate-sensitive potato were subjected to PCR and Southern hybridization to determine possible occurrence of glufosinate-resistant soil bacteria and to detect the bar (phosphinothricin acetyltransferase) gene, conferring tolerance to glufosinate. The bar gene was not detected from genomic DNAs extracted at different time intervals from the soil samples, which had been treated with the leaf tissue of either transgenic or non-transgenic potato for 2 to 8 weeks. In addition, the level of glufosinate-resistant bacteria isolated from the soil samples treated with the leaf tissue of transgenic potato was similar to that of the samples treated with non-transgenic potato after 4 months of incubation at $25^{\circ}C$. The bar gene was not detected in the genomic DNAs extracted from colonies growing on the plate containing glufosinate, indicating that the bacteria could acquire the resistant phenotype to glufosinate by another mechanism without the uptake of the bar gene from glufosinate-tolerant potato.

Keywords

References

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