Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Microbial Conversion of Major Ginsenoside $Rb_1$ to Pharmaceutically Active Minor Ginsenoside Rd

  • Kim Myung Kyum (Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University) ;
  • Lee Jun Won (Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University) ;
  • Lee Ki Young (Department of Food & Biotechnology, Hoseo University) ;
  • Yang Deok-Chun (Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University)
  • Published : 2005.10.01
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Abstract

More than seventy strains of aerobic bacteria showing ${\beta}$-glucosidase activity were isolated from a ginseng field, using a newly designed Esculin-R2A agar, and identified by their 16S rRNA gene sequences. Of these microorganisms, twelve strains could convert the major ginsenoside, $Rb_1$, to the pharmaceutically active minor ginsenoside Rd. Three strains, Burkholderia pyrrocinia GP16, Bacillus megaterium GP27 and Sphingomonas echinoides GP50, were phylogenetically studied, and observed to be most potent at converting ginsenoside $Rb_1$ almost completely within 48 h, as shown by TLC and HPLC analyses.

Keywords

References

  1. Bae, E.-A., S.-Y. Park, and D.-H. Kim. 2000. Constitutive $\beta$-glucosidases hydrolyzing ginsenoside $Rb_1$ and $Rb_2$ from human intestinal bacteria. Biol. Pharm. Bull. 23, 1481-1485 https://doi.org/10.1248/bpb.23.1481
  2. Chen, S.-W., L.-J. Wang, Y. Wang, and B.-X. Wang. 2001. Effects of ginsenoside $Rb_1$ and Rd on learning and memory function of mice. Chinese J. Pharmacol. Toxicol. 15, 330-332
  3. Choi, S.-S., J.-K. Lee, E.-J. Han, K.-J. Han, H.-K. Lee, J. Lee, and H.-W. Suh. 2003. Effect of ginsenoside Rd on nitric oxide system induced by lipopolysaccharide plus TNF-$\alpha$ in C6 rat glioma cells. Arch. Pharm. Res. 26, 375-382 https://doi.org/10.1007/BF02976694
  4. Coskun, H. and E. Ondul 2004. Free fatty acid accumulation by mesophilic lactic acid bacteria in cold-stored milk. J. Microbiol. 42, 133-138
  5. Dong, A., M. Ye, H. Guo, J. Zheng, and D. Guo. 2003. Microbial transformation of ginsenoside $Rb_1$ by Rhizopus stolonifer and Curvularia lunata. Biotechnol. Lett. 25, 339-344 https://doi.org/10.1023/A:1022320824000
  6. Felsenstein, J. 1985. Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39, 783-791 https://doi.org/10.2307/2408678
  7. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95-98
  8. Kim, M.-W., S.-R. Ko, K.-J. Choi, and S.-C. Kim. 1987. Distribution of saponin in various sections of Panax ginseng root and changes of its contents according to root age. Korean J. Ginseng Sci. 11, 10-16
  9. Kim, M.K., W.-T. Im, H. Ohta, M. Lee, and S.-T. Lee. 2005. Sphingomonas granuli sp. nov., a $\beta$-glucosidase-producing bacterium in the family Sphingomonadaceae in $\alpha$-4 subclass of the Proteobacteria. J. Microbiol. 43, 152-157
  10. Kimura, M. 1983. The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press
  11. Kumar, S., K. Tamura, I.B. Jakobsen, and M. Nei. 2001. MEGA2: Molecular Evolutionary Genetics Analysis software. Bioinformatics 17, 1244-1245 https://doi.org/10.1093/bioinformatics/17.12.1244
  12. Lee, J.-K., S.-S. Choi, H.-K. Lee, K.-J. Han, E.-J. Han, and H.-W. Suh. 2003. Effects of ginsenoside Rd and decursinol on the neurotoxic responses induced by kainic acid in mice. Planta Med. 69, 230-234 https://doi.org/10.1055/s-2003-38475
  13. Park, S.-Y., E.-A. Bae, J.H. Sung, S.-K. Lee, and D.-H. Kim. 2001. Purification and characterization of ginsenoside $Rb_1$-metabolizing $\beta$-glucosidase from Fusobacterium K-60, a human intestinal anaerobic bacterium. Biosci. Biotechnol. Biochem. 65, 1163-1169 https://doi.org/10.1271/bbb.65.1163
  14. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Bio. Evol. 4, 406-425
  15. Shi, Q, Q. Hao, J. Bouissac, Y. Lu, S. Tian, and B. Luu. 2005. Ginsenoside- Rd from Panax notoginseng enhances astrocyte differentiation from neural stem cells. Life Sci. 76, 983-995 https://doi.org/10.1016/j.lfs.2004.07.026
  16. Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24, 4876-4882
  17. Weisburg, W.G., S.M. Barns, D.A. Pelletier, and D.J. Lane. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173, 697-703
  18. Yokozawa, T. and E. Dong. 2001. Role of ginsenoside-Rd in cisplatin- induced renal injury: special reference to DNA fragmentation. Nephron 89, 433-438 https://doi.org/10.1159/000046116
  19. Yokozawa, T, A. Satoh, and E.J. Cho. 2004. Ginsenoside-Rd attenuates oxidative damage related to aging in senescence-accelerated mice. J. Pharm. Pharmacol. 56, 107-113 https://doi.org/10.1211/0022357022449
  20. Yokozawa, T. and S. Owada. 1999. Effects of ginsenoside Rd cephaloride- induced renal disorder. Nephron 81, 200-207 https://doi.org/10.1159/000045277
  21. Yokozawa, T. and Z.W. Liu. 2000. The role of ginsenoside Rd in cisplatin-induced acute renal failure. Ren. Fail. 22, 115-127 https://doi.org/10.1081/JDI-100100858
  22. Yokozawa, T., Z.W. Liu, and E. Dong. 1998. A study of ginsenoside- Rd in a renal ischemia-reperfusion model. Nephron 78, 201-206 https://doi.org/10.1159/000044911
  23. Yoon, K.Y., E.E. Woodams, and Y.D. Hang 2004. Probiotication of tomato juice by lactic acid bacteria. J. Microbiol. 42, 315-318
  24. Yu, H., H. Liu, C. Zhang, D. Tan, M. Lu, and F. Jin. 2004. Purification and characterization of gypenoside-$\alpha$-L-rhamnosidase hydrolyzing gypenoside-5 to ginsenoside Rd. Process Biochem. 39, 861-867 https://doi.org/10.1016/S0032-9592(03)00196-1
  25. Zeng, S., Y.-Y. Guan, D.-Y. Liu, H. He, W. Wang, Q.-Y. Qiu, X.-R. Wang, and Y.-D. Wang. 2003. Synthesis of 12-epi-ginsenoside Rd and its effects on contractions of rat aortic rings. Chinese Pharmacol. Bull. 19, 282-286
  26. Zhang, C., H. Yu, Y. Bao, L. An, and F. Jin. 2002. Purification and characterization of ginsenoside-$\alpha$-arabinofuranase hydrolyzing ginsenoside Rc to Rd from the fresh root of Panax ginseng. Process Biochem. 37, 793-798 https://doi.org/10.1016/S0032-9592(01)00275-8