Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

The Effect of Mixed Amino Acids on Nitrate Uptake and Nitrate Assimilation in Leafy Radish

  • Published : 2005.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The objective of the present work was to determine the corresponding uptake and assimilation of ${NO_3}^-$ in roots and shoots of leafy radish by applying of mixed amino acids (MAA). The amino acids used in this experiment were alanine (Ala), ${\beta}-alanine\;({\beta}-Ala)$, aspartic acid (Asp), asparagines (Asn), glutamic acid (Glu), glutamine (Gln), and glycine (Gly). Leafy radish was grown by conventional fertilization with macro- and micronutrients under controlled conditions. The 15-day-old seedlings were treated 0, 0.3 and 3.0 mM of MAA containing 5 mM ${NO_3}^-$ in growth medium. Nitrate uptake was determined by following ${NO_3}^-$ depletion from the uptake solution. The activity of the enzymes related to the process of ${NO_3}^-$ reduction (NR: nitrate reductase; NiR: nitrite reductase; GS: glutamine synthetase) and the content of ${NO_2}^-\;and\;{ND_3}^-$ were analyzed in shoots and roots. The results of this study showed that ${NO_3}^-$ uptake was inhibited 38% with treatment of 0.3 mM of MAA. However, there was more than three times increase of N03- uptake in 3.0 mM MAA. In addition, the enzymatic activities were positively affected by the high MAA rate. Finally, the ${NO_3}^-$ content was increased slightly both in shoots and roots of leafy radish by MAA treatments.

Keywords

References

  1. Sivasankar, S., Rothstein, S., and Oaks A. (1997) Regulation of the accumulation and reduction of nitrate by nitrogen and carbon metabolites in maize seedlings. Plant Physiol. 114, 583-589
  2. Taiz, L. and Zeiger, E. (2002) Plant Physiology. 3rd Edition. Sinauer Associates, Inc., Sunderland, USA, P. 260-287
  3. Campbell, W.H. (1999) Nitrate reductase structure, function and regulation: bridging the gap between Biochemistry and Physiology. Annu. Rev. Plant Physiol. Mol. Biol. 50, 277-303 https://doi.org/10.1146/annurev.arplant.50.1.277
  4. Ford, B.G. (2000) Nitrate trnsporters in plants: structure, function and regulation. Biochimica et Biophysic Acta. 1465, 219-235 https://doi.org/10.1016/S0005-2736(00)00140-1
  5. Chapin, F.S., Moilainen, L., and Kielland, K. (1993) Preferential use of organic nitrogen by a non-mycorrhizal arctic sedge. Nature, 361, 150-153 https://doi.org/10.1038/361150a0
  6. Matsumoto, S., Ae, N., and Yamagata, M. (2000) Possible direct uptake of organic nitrogen from soil by chingensai (Brassica campestris L.) and carrot (Daucus carota L.). Soil biology & Biochemistry. 32, 1301-1310 https://doi.org/10.1016/S0038-0717(00)00048-1
  7. Persson, J. and Nasholm, T. (2002) Regulation of amino acid uptake in conifers by exogenous and endogenous nitrogen. Planta. 215, 639-644 https://doi.org/10.1007/s00425-002-0786-5
  8. Persson, J. and Nasholm T. (2001) Amino acid uptake: a Widespread ability among boreal forest plants. Ecol Lett. 4, 434-438 https://doi.org/10.1046/j.1461-0248.2001.00260.x
  9. Muller, B. and Touraine, B. (1992) Inhitition of $NO_3^-$ uptake by various phloem-translocated amino acids in soybean seedling. J. Exp. Bot. 43, 617-623 https://doi.org/10.1093/jxb/43.5.617
  10. Rodgers, C.O. and Barneix, A.J. (1993) The effect of amino acids and amides on the regulation of nitrate uptake by wheat seedlings. J. Plant Nutr. 16, 337-348 https://doi.org/10.1080/01904169309364535
  11. Li, X.Z., Larson, D.E., Glibetic, M., and Oaks, A. (1995) Effect of glutamine on the induction of nitrate reductase. Physiol plant. 93, 740-744 https://doi.org/10.1111/j.1399-3054.1995.tb05125.x
  12. Padgett, P.E. and Leonard, R.T. (1996) Free amino acid levels and the regulation of nitrate uptake in maize cell suspension cultures. J. Exp. Bot. 47, 871-883 https://doi.org/10.1093/jxb/47.7.871
  13. Ivashikian, N.V. and Sokolov, O. A. (1997) Regulation of nitrate, nitrite, ammonium and glutamate. Plant Science. 123, 29-37 https://doi.org/10.1016/S0168-9452(96)04566-9
  14. Tillard, P., Passama, L., and Gojon, A. (1998) Are phloem amino acids involved in the shoot to root control of $NO_3^-$ uptake in Ricinus communis plants? J. Exp. Bot. 49, 1371-1379 https://doi.org/10.1093/jexbot/49.325.1371
  15. Aslam, M., Travis, R.L., and Rains, D.W. (2001) Differential effect of amino acids on nitrate uptake and reduction systems in barley roots. Plant Science. 160, 219-228 https://doi.org/10.1016/S0168-9452(00)00391-5
  16. Pal'ove-Balang, P. (2002) Role of nitrogen metabolites on the regulation of nitrate uptake in maize seedlings. Acta Biologica Szegediensis. 46, 177-178
  17. Cerezo, M., Flors, V., Legaz, F., and Garcia-Agustin, P. (2000) Characterization of the low affinity transport system for $NO_3^-$ uptake by Citrus roots. Plant Science. 160, 95-104 https://doi.org/10.1016/S0168-9452(00)00363-0
  18. Daniel-Vedele, F., Filleur, S., and Caboch, M. (1998) Nitrate transport: a key step in nitrate assimilation. Current Opinion in Plant Biology. 1, 235-239 https://doi.org/10.1016/S1369-5266(98)80110-6
  19. Deng, M.D., Moureaux, T., Cherel, I., Boutin, J.P., and Caboche, M. (1991) Effects of nitrogen metabolites on the regulation and circadian expression of tobacco nitrate reductase. Plant Physiol. Biochem. 29, 139-247
  20. Vicentz, M., Moureaux, T., Leydecker, M.T., Vaucheret, H., and Caboche, M. (1993) Regulation of nitrate and nitrite reductase expression in Nicotiana plumbaginifolia leaves by nitrogen and carbon metabolites. Plant J. 3, 315-324 https://doi.org/10.1046/j.1365-313X.1993.t01-16-00999.x
  21. Kim, Y. S. (2002) The effect of mixed amino acid on nitrate uptake in rice, pea, cucumber and red pepper. Master of Agriculture Degree Thesis, Department of Agricultural Chemistry, College of Agriculture and Biotechnology, Chungnam National University, Daejon, Korea
  22. Ruiz, J. M., Rivero, R. M., Garcia, P. C., Baghour, M., and Romero R. (1999) Role of $CaCl_2$in nitrate assimilation in leaves and roots of tobacco plants (Nicotiana tabacum L.). Plant Science. 141, 107-115 https://doi.org/10.1016/S0168-9452(98)00230-1
  23. Cataldo, D.A., Haroon, M., Schrader, L.E., and Young, V.L. (1975) Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Comm. Soil Sci. Plant Anal. 6, 71-80 https://doi.org/10.1080/00103627509366547
  24. Kaiser, J.J. and Lewis, O.A.H. (1984) Nitrate reductase and glutamine synthetase activity in leaves and roots of nitrate fed Helianthus annuus L. Plant Soil. 70, 127-130
  25. Gunes, A., Post, W.H.K., Kirkby, E.A., and Akas, M. (1994) Influence of partial replacement of nitrate by amino acid nitrogen or urea in the nutrient medium on nitrate accumulation in NFT grown winter lettuce. J. Plant Nutr. 17, 1929-1938 https://doi.org/10.1080/01904169409364855
  26. Gunes, A., Inal, A., and Aktas, M. (1996) Reducing nitrate content of NFT grown winter onion plants (Allium cepa L.) by partial replacement of $NO_{3}$ with amino acid in nutrient solution. Scientia Horticulturae, 65, 203-208 https://doi.org/10.1016/0304-4238(95)00864-0
  27. Chen, G. and Gao X. (2002) Effect of partial replacement of nitrate by amino acid and urea on nitrate content of nonheading Chinese cabbage and lettuce in hydroponics (Chinese). Scientia Agricultura Sinica, 35, 187-191
  28. Wang, H., Wu, L., and Tao, Q. (2004) Influence of partial replacement of nitrate by amino acids on nitrate accumulation of pakchoi (Brassica chinensis L.) (Chinese). China Environmental Science, 24, 19-23
  29. Radin, J.W. (1975) Differential regulation of nitrate reductase induction in roots and shoots of cotton plants. Plant Physiol. 55, 178-182 https://doi.org/10.1104/pp.55.2.178
  30. Radin, J.W. (1977) Amino acid interactions in the regulation of nitrate reductase induction in cotton roots tips. Plant Physiol. 60, 467-469 https://doi.org/10.1104/pp.60.4.467
  31. Oaks, A., Stulen, I., and Boesel, I. (1979) Influence of amino acids and ammonium on nitrate reduction in com seedlings. Can. J. Bot. 57, 1824-1829 https://doi.org/10.1139/b79-227
  32. Oaks, A. (1994) Primary nitrogen assimilation in higher plants and its regulation. Can. J. Bot. 72, 739-750 https://doi.org/10.1139/b94-094
  33. Bameix, A.J. and Causin, H.P. (1996) The central role of amino acids on nitrogen utilization and plant growth. J. Plant Physiol. 149, 358-362 https://doi.org/10.1016/S0176-1617(96)80134-9
  34. Pessarakli, M. (2002) Handbook of plant and crop physiology. 2nd Edition. Marcel Dekker, Inc., New York, USA, P. 390
  35. Barneix, A.J., James, D.M., Watson, E.F., and Hewitt, E.J. (1984) Some effects of nitrate abundance and starvation on metabolism and accumulation of nitrogen in barley (Hordeum vulgare L.) cv. Sonja. Planta. 162, 465-476

Cited by

  1. Evaluation of the Role of Mixed Amino Acids in Nitrate Uptake and Assimilation in Leafy Radish by Using 15N-Labeled Nitrate vol.7, pp.10, 2008, https://doi.org/10.1016/S1671-2927(08)60164-9
  2. Effect of Amino Acid Fertilization on Nitrate Assimilation of Leafy Radish and Soil Chemical Properties in High Nitrate Soil vol.39, pp.1-2, 2007, https://doi.org/10.1080/00103620701759301
  3. Effects of Two Amino Acid Fertilizers on Growth of Creeping Bentgrass and Nitrogen Uptake vol.3, pp.3, 2014, https://doi.org/10.5660/WTS.2014.3.3.246
  4. Growth and Quality Changes of Creeping Bentgrass by Application of Keratin Amino Acid Fertilizer vol.5, pp.4, 2016, https://doi.org/10.5660/WTS.2016.5.4.260