Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Effects of Simulated Acid Rain on Growth and Antioxidant System in French Marigold (Tagetes patula L.)

인공산성비가 만수국(Tagetes patula L.)의 생육 및 항산화 작용에 미치는 영향

  • Published : 2005.06.30
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Abstract

This study was conducted to investigate the effect of simulated acid rain (SAR) on growth and antioxidant system in french marigold (Tagetes patula L.). Plants were subjected to four levels of SAR (pH 5.6, 4.0, 3.0, 2.0) in the growth chambers for 2 weeks. SAR decreased both plant height and plant dry weight of french marigold. As the pH levels decreased from 5.6 to 2.0, the content of MDA highly increased linearly. The ratios of dehydroascorbate/ascorbate and oxidized glutathione/reduced glutathione were significantly increased with decreasing pH levels. The enzyme (superoxide dismutase, ascorbate peroxidase etc.) activities of the plant affected by SAR were increased as the pH decreased. Based on the results, SAR caused oxidative stress in french marigold and resulted in significant reduction in plant growth. Biochemical protection responses might be activated to prevent the plant from damaging effects of oxidative stress generated in SAR.

도시의 화단 및 꽃길 조성용으로 많이 이용되고 있는 만수국을 대상으로 산성비에 의한 식물 피해 양상을 조사하고, 산성비 처리가 활성산소 생성에 의한 산화 스트레스를 일으키는지를 조사함과 동시에 산성비에 대한 식물의 생화학적 방어반응을 조사하기 위하여 여러 농도의 인공 산성비(pH 2.0, 3.0, 4.0, 5.6)를 제조하여 실험을 수행하였다. 산성비의 pH가 낮을수록 생육 피해는 심하게 나타났으며 pH 3.0 이하의 처리에 의해 암회색 또는 적갈색의 괴사 반점이 생성되었다. MDA 함량은 pH 2.0 처리에서 약 2배 정도의 증가를 나타내었다. 산성비의 $H^+$ 부하량 증가에 따라 DHA/AsA 및 GSSG/GSH의 비율이 증가하였다. SOD, AP, GP 등의 항산화효소 활성도 산성비의 $H^+$ 부하량의 증가에 따라 크게 증가하는 것으로 나타났다. 이상의 결과로 볼 때 산성비는 만수국 식물에 활성산소 생성에 의한 산화스트레스가 일으키며, 이를 무독화하기 위해 식물의 생화학적 방어반응이 작용하는 것으로 사료된다.

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