Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Effects of Low Dose Gamma-irradiation on Alleviation of Salt Stress in Rice (Oryza sativa L.) Seedling

저선량 감마선 조사가 벼 유묘의 염 스트레스 경감에 미치는 영향

  • Baek, Myung-Hwa (Division of Radiation Application Research, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Hong (Division of Radiation Application Research, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Wi, Seung-Gon (Division of Radiation Application Research, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, In-Jung (Department of Agronomy, Kyungpook National University) ;
  • Lee, Kyu-Seong (National Institute of Crop Science, R.D.A.) ;
  • Kim, Jae-Sung (Division of Radiation Application Research, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 백명화 (한국원자력연구소 방사선이용연구부) ;
  • 김진홍 (한국원자력연구소 방사선이용연구부) ;
  • 위승곤 (한국원자력연구소 방사선이용연구부) ;
  • 이인중 (경북대학교 농학과) ;
  • 이규성 (농촌진흥청 작물과학원) ;
  • 김재성 (한국원자력연구소 방사선이용연구부)
  • Published : 2005.06.30
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Abstract

To investigate alleviation effects of salt stress by low dose gamma-irradiation on seedling growth of rice (Oryza shrive L), in plants of two rice cultivars (Ilpumbyeo and Gancheokbyeo), seeds were irradiated with the different doses $(0{\sim}32\;Gy)$ of gamma-ray by irradiator ($^{60}Co$, ca.150 TBq of capacity, AECL). The salt stress caused a remarkable decrease of seedling growth, particularly for dry weight. In addition, relative growth rate was decreased over 50% by the salt stress. However, the salt stress-induced inhibition of growth was alleviated by gamma-irradiation. Especially, the relative growth rate of shoot showed the reduction effect over 30% at 4 Gy-irradiated groups as compared with that of control. Also, electrolyte leakage and relative water content were damaged, respectively 4 times and 30% by the salt stress but the damages were alleviated by the gamma-irradiation. These alleviation effects were observed similarly in both the cultivars tested. These results suggest that the low dose gamma-irradiation may have a better protection or the salt stress.

염 스트레스 하에서 벼 생육에 대한 저선량 방사선의 경감효과를 확인하기 위해 일품벼와 간척벼 두 품종에 저선량 감마선을 0-32 Gy 수준으로 조사하였다. 염 처리에 따른 생장반응은 무 처리구에 비해 두 품종 모두 염 처리구에서 생육이 감소하였으며, 초장과 생체중보다 건물중에서 더 많은 감소를 보였다. 이외에도 초기건량에 대한 단위시간당 건물의 증가를 나타내는 상대생장율을 관찰한 결과, 전체 상대생장율이 염 처리구에서 50% 이상 억제되었다. 그러나 이러한 생육억제는 저선량 방사선에 의해 경감됨을 확인할 수 있었다. 특히, 줄기 상대생장율의 경우 염 처리시 4 Gy 조사구가 대조구보다 30% 이상의 경감효과를 보였다. 또한 염 스트레스에 대한 조직의 막투과성 정도는 염 처리에 의해 4배 정도 피해를 보였으나 4 Gy 조사구에서 10% 이상의 경감효과를 보였으며, 상대수분함량도 30% 정도의 감소를 보인 염 처리구에서 저선량 방사선의 효과를 확인 할 수 있었다. 이러한 결과를 통해, 저선량 방사선이 염 스트레스에 대한 저해 효과를 경감시키는 것으로 사료된다.

Keywords

References

  1. Flowers, T. J. and Yeo, A. R. (1995) Breeding for salinity resistance in crop plants: where next?, Aust. J. Plant Physiol. 22, 875-884 https://doi.org/10.1071/PP9950875
  2. Choung, J. I., Yu, S. J., Oh, M. K., Baek, N. H., Ko, J. K. and Lee, J. K. (2002) Varietal responses of rice growth and yield to soil salt content, Kor. J. Crop Sci. 47, 422-426
  3. Lee, I. S. (2002) In vitro selection and characterizations of gamma radiation-induced salt tolerance lines in rice, Ph.D. Thesis, Chungnam National University, Daejon
  4. Balasuburamanian, V. and Rac, S. (1977) Physiology basis of salt tolerance in rice, PI, physiol. section, Tamil Nagada Agri. Univ. India. 26, 291-294
  5. Luckey, T. D. (1980) Hormesis with ionizing radiation, CRC press. Inc. Boca Raton, Florida
  6. Kim, J. S. and Lee, Y. B. (1998) Ionizing radiation hormesis in crops, Kor. J. Environ. Agri. 17, 76-85
  7. Kim, J. S., Lee, Y. K., Back, M. W., Lee, Y. B. and Park, Y. S. (1999) Influence of the low dose ${\gamma}$-ray radiation on the old seed germination and growth of chinese cabbage, Kor. J. Environ. BioI. 17, 11-15
  8. Lee, E. K., Kim, J. S., Lee, Y. K. and Lee, Y. B. (1998) Effect of low dose ${\gamma}$-ray irradiation on the germination and growth in red pepper (Capsicum annuum L.), J. Kor. Soc. Hort. Sci. 39, 670-675
  9. Campos, F. F. and Velasco, E. G. (1962) Comparative effect of $cobalt^{60}$ on plant character of two recommended Philippine lowland rice varieties, Philipp. Agric. 46, 93-95
  10. Kaiwdl, K. and Rosner, M. (1965) The accelerating effect of small radiation doses on plant, Bayerisches Landwirtschaftliches Jahrbuch 42, 11-29
  11. Vlasyuk, P. A. (1964) Effect of ionizing radiation on the physiological-biochemical properties and metabolism of agricultural plants, Inst. Fiziol. Biokhim, Rast. SSR. 24-31
  12. Kim, J. S., Lee, E. K., Back, M. H., Park, H. S. and Kim, K. H. (1999) Effect of low dose of gamma radiation on the growth of groundnut (Arachis hypogaea L.), Kor. J. Environ. Biol. 17, 257-261
  13. Kim, J. S., Lee, Y. K., Park, H. S., Back, M. H. and Kim, D. H. (2000) Influence of low dose gamma radiation on the growth of maize (Zea mays L.) varieties, Kor. J. Environ. Agri. 19, 328-331
  14. Kim, J. S., Lee, E. K., Lee, Y. K. and Lee, Y. B. (1999) Influence of artificial acid rain on the growth of soybean irradiated with low dose gamma radiation, Kor. J. Environ. Agri. 18, 245-249
  15. Lee, H. Y., Kim, J. S., Baek, M. H., Park, S. C. and Park, Y. I. (2002) Effects of low dose ${\gamma}$-radiation on photosynthesis of red pepper (Capsicum annuum L.) and the reduction of photoinhibition, Kor. J. Environ. Agri. 21, 83-89 https://doi.org/10.5338/KJEA.2002.21.2.083
  16. Lee, H. Y., Kim, J. S., Baek, M. H., Yoo, J. C. and Kwon, S. T. (2003) Effects of low dose ${\gamma}$ irradiation on physiological activities of radish (Raphanus sativus) during early growth and reduction of ultraviolet-B stress, Kor. J. Horti. Sci. 44, 314-320
  17. Niels, W. H. and Roger, J. B. (1970) Manual on Radiation Dosimetry, Mard Dekker Inc., New York
  18. Maribel, L., Dionisio, S. and Satoshi, T. (1998) Antioxidant responses of rice seedlings to salinity stress, Plant Sci. 135, 1-9 https://doi.org/10.1016/S0168-9452(98)00025-9
  19. Barr, H. D. and Weatherley, P. E. (1962) A reexamination of the relative turgidity technique for estimating water deficit in leaves, Aust. J. Biol. Sci. 15, 413-428 https://doi.org/10.1071/BI9620413
  20. Chuan, C. L. and Ching, H. K. (1996) Proline accumulation is associated with inhibition of rice seedling root growth caused by NaCl, Plant Sci. 114, 121-128 https://doi.org/10.1016/0168-9452(96)04323-3
  21. Aeschxbacher, R. A., Schiefelbein, J. W. and Benfey, P. N. (1994) The genetic and molecular basis of root development, Ann. Rev. of Plant Physiol. and Plant Mol. BioI. 45, 25-45 https://doi.org/10.1146/annurev.pp.45.060194.000325
  22. Yeo, A. R. and Flowers. T. J. (1982) Accumulation and localization of sodium ions within the shoots of rice (Oryza sativa L.) varieties differing in salinity resistance, Physiol. Plant 56, 189-195 https://doi.org/10.1111/j.1399-3054.1982.tb00323.x
  23. Hwangbo, J. K, Kim, J. S., Lim, J. H., Baek, M. H. and Chung, B. Y. (2003) Alterations in seed vigour and viability of soybean related with accelerated seed aging and low dose gamma irradiation, Kor. J. Crop Sci. 48, 334-338
  24. Kim, J. S., Lee, E. K., Song, J. Y., Kim, H. K. and Lee, Y. B. (2000) Induction of resistance against Phyphthora Blight of pepper by low dose gamma ray radiation, Kor. J. Environ. Biol. 18, 47-51
  25. Dubey, R. S. (1994) Protein synthesis by plants under stressful conditions, In: M. Pessarakli (Ed), Handbook of Plant a Crop Stress, Marcel Dekker, New York. pp 277-299
  26. Shin, S. H. (2002) Physiological and anatomical characteristics of salt-tolerance of rice (Oryza sativa L.) varieties, Ph.D. Thesis, Chonnam National University, Kwangju
  27. Gadallah, M. A. A. (1999) Effect of proline and glycine-betain on Vicia faba responses to salt stress, Biol. Plant. 42, 247-279 https://doi.org/10.1007/BF03030486