Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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THE EFFECT OF RHBMP-2 IN HUMAN BONE MARROW-DERIVED STEM CELLS AS OSTEOGENIC INDUCERS

사람의 골수 줄기 세포로부터의 골세포 분화 과정에서 BMP-2가 미치는 영향과 그에 따른 분화 유전자의 발현 비교 연구

  • Kim, In-Sook (Dental Research Institute, Seoul National University) ;
  • Zhang, Yu-Lian (Dental Research Institute, Seoul National University) ;
  • Cho, Tae-Hyung (Dental Research Institute, Seoul National University) ;
  • Lee, Kyu-Back (Dept. of Biomedical Engineering, College of Medicine, Brain Korea 21 Program for Biomedical Science, Korea Artificial Organ Center, Korea University) ;
  • Park, Yong-Doo (Dept. of Biomedical Engineering, College of Medicine, Brain Korea 21 Program for Biomedical Science, Korea Artificial Organ Center, Korea University) ;
  • Rho, In-Sub (Chemical Engineering Department, Seoul National University of Technology) ;
  • Weber, F. (Lab. for Osteology, Dept. of Craniomaxillofacial Surgery, University Hospital Zurich) ;
  • Hwang, Soon-Jung (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Kim, Myung-Jin (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Lee, Jong-Ho (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University)
  • 김인숙 (서울대학교 치학연구소) ;
  • 장옥련 (서울대학교 치학연구소) ;
  • 조태형 (서울대학교 치학연구소) ;
  • 이규백 (고려대학교 의과대학 의공학교실, BK21 의과학사업단, 한국인공장기센터) ;
  • 박용두 (고려대학교 의과대학 의공학교실, BK21 의과학사업단, 한국인공장기센터) ;
  • 노인섭 (서울산업대학교 화학공학과) ;
  • ;
  • 황순정 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 김명진 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 이종호 (서울대학교 치과대학 구강악안면외과학교실)
  • Published : 2005.01.31
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Abstract

It is commonly acknowledged that bone morphogenic protein (BMP-2) functions as a potential osteogenic inducer in bone formation. Recently, several papers reported that bone marrow-derived stem cell (BMSC) from human is not responsive to BMP-2 in comparison to high capacity of BMP-2 in the osteoinduction of stromal cell derived from bone marrow of rodent animals such as rat or mouse. In this study, we characterized BMSC derived from 11 years old donor for the responsiveness to rhBMP-2, dexamethasone (Dex) and 1,25-dihydroxyvitamin D (vitamin D), in order to analyze their function in the early osteogenesis. The effect of over mentioned agents was evaluated by means of assessing alkaline phosphatase (ALP) activity/staining, RT-PCR analysis and von Kossa staining. In addition, we analyzed the meaning of expressed several osteoblastic markers such as alkaline phosphatase, collagen typeI, osteopontin, bone sialoprotein and osteocalcin with relation to either differentiation or mineralization. Only in the presence of Dex, human BMSC could commit osteoblastic differentiation and matrix mineralization, and either BMP-2 or vitamin D treatment was not able to induce. But BMP-2 or Vitamin D showed potential synergy effect with Dex. ALP and bone sialoprotein were clearly expressed in response of Dex treatment compared to weak expression of osteopontin in early osteogenesis. Therefore, we expect that this study will contribute partly to elucidiating early osteogenesis mechanism in human, but variations among bone marrow donors must be considered through further study.

Keywords

References

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