Journal of Microbiology

  • 제43권4호
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  • Pages.325-330
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  • 2005
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Isolation and Characterization of a Rhodococcus Species Strain Able to Grow on ortho- and para-Xylene

  • Jang Jung Yeon (Department of Biology, Yonsei University) ;
  • Kim Dockyu (Department of Biology, Yonsei University, Microbial Resources Bank, Microbial Genomics and Applications Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Bae Hyun Won (Department of Biology, Yonsei University) ;
  • Choi Ki Young (Department of Biology, Yonsei University) ;
  • Chae Jong-Chan (Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University) ;
  • Zylstra Gerben J. (Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University) ;
  • Kim Young Min (Department of Biology, Yonsei University) ;
  • Kim Eungbin (Department of Biology, Yonsei University)
  • 발행 : 2005.08.01
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초록

Rhodococcus sp. strain YU6 was isolated from soil for the ability to grow on o-xylene as the sole carbon and energy source. Unlike most other o-xylene-degrading bacteria, YU6 is able to grow on p-xylene. Numerous growth substrate range experiments, in addition to the ring-cleavage enzyme assay data, suggest that YU6 initially metabolizes 0- and p-xylene by direct aromatic ring oxidation. This leads to the formation of dimethylcatechols, which was further degraded largely through meta-cleavage path-way. The gene encoding meta-cleavage dioxygenase enzyme was PCR cloned from genomic YU6 DNA using previously known gene sequence data from the o-xylene-degrading Rhodococcus sp. strain DK17. Subsequent sequencing of the 918-bp PCR product revealed a $98\%$ identity to the gene, encoding meth-ylcatechol 2,3-dioxygenase from DK17. PFGE analysis followed by Southern hybridization with the catechol 2,3-dioxygenase gene demonstrated that the gene is located on an approximately 560-kb megaplasmid, designated pJY J1

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